Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2008
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 11:00–12:50 Bpt,01013
Prerequisites
The course is for the students with interest about molecular biotechnology. Prerequisites are exams from Molecular biology(Bi4020) and General microbiology(Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Definition of molecular biotechnology and its emergence. Impact of recombinant DNA technology on molecular biotechnology. Procedures enabling gene transfer from one organism into an other (vectors for cloning of DNA fragments of different lengths, construction and screening of gene and genome libraries, cDNA and metagenomic libraries). Manipulation of gene expression in prokaryotes (the use of strong and regulatable promotors, expression vectors, integration vectors, vectors for construction of fusion proteins). Heterologous protein production in eukaryotic cells. Microbial molecular biotechnology and its use in molecular diagnostics, in production of small molecules, therapeutic proteins and vaccines, in bioremediation and in biomass utilisation. The next use of transgenic microorganisms, plants and animals. Regulating of GMO use. This course is supplemented by practical course Bi7430c.
At the end of this course, student should be able to explain the use of recombinant DNA technology for the construction of genetic modified organisms producing commercial products. Using aquired data he will be able to understand new genetic modified organisms.
Syllabus
  • Relationship between chemical production and biotechnologies.
  • Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
  • Recombinant DNA technology. Plasmid cloning vectors and their use.
  • Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
  • Vectors for cloning large fragments of DNA.
  • Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
  • Increasing of protein stability. Fusion proteins.
  • Translation expression vectors. DNA integration into host chromosome.
  • Heterologous protein production in eukaryotic cells.
  • Directed mutagenesis and protein engineering.
  • Microbial molecular biotechnology. Molecular diagnostics.
  • Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
  • Production of small biological molecules, antibiotics and biopolymers.
  • Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
  • Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.
Literature
  • ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
  • GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info
Assessment methods
Lectures are enclosed by test and oral exam.
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/Bi7430