PřF:Bi7430 Molecular biotechnology - Course Information
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 11:00–12:50 Bpt,01013
- Prerequisites
- The course is for the students with interest about molecular biotechnology. Prerequisites are exams from Molecular biology(Bi4020) and General microbiology(Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Definition of molecular biotechnology and its emergence. Impact of recombinant DNA technology on molecular biotechnology. Procedures enabling gene transfer from one organism into an other (vectors for cloning of DNA fragments of different lengths, construction and screening of gene and genome libraries, cDNA and metagenomic libraries). Manipulation of gene expression in prokaryotes (the use of strong and regulatable promotors, expression vectors, integration vectors, vectors for construction of fusion proteins). Heterologous protein production in eukaryotic cells. Microbial molecular biotechnology and its use in molecular diagnostics, in production of small molecules, therapeutic proteins and vaccines, in bioremediation and in biomass utilisation. The next use of transgenic microorganisms, plants and animals. Regulating of GMO use. This course is supplemented by practical course Bi7430c.
At the end of this course, student should be able to explain the use of recombinant DNA technology for the construction of genetic modified organisms producing commercial products. Using aquired data he will be able to understand new genetic modified organisms. - Syllabus
- Relationship between chemical production and biotechnologies.
- Molecular biotechnology, emergence, definition. Molecular biotechnology biological systems.
- Recombinant DNA technology. Plasmid cloning vectors and their use.
- Creating and screening of gene libraries. Cloning DNA sequences that encode eucaryotic proteins.
- Vectors for cloning large fragments of DNA.
- Manipulation of gene expression in prokaryots. Strong and regulatable promotors.
- Increasing of protein stability. Fusion proteins.
- Translation expression vectors. DNA integration into host chromosome.
- Heterologous protein production in eukaryotic cells.
- Directed mutagenesis and protein engineering.
- Microbial molecular biotechnology. Molecular diagnostics.
- Production of therapeutic proteins. Nucleic acids as therapeutics. Vaccines - subunit, attenuated, vector.
- Production of small biological molecules, antibiotics and biopolymers.
- Bioremediation and utilisation of biomass. Plant growth promoting bacteria. Bacterial insecticides.
- Transgenic plants, their construction and use. Transgenic animals, their construction and use. Regulating of GMO use.
- Literature
- ROSYPAL, Stanislav. Úvod do molekulární biologie (Introduction to molecular biology). Čtvrté inovované vydání. Brno: Prof. RNDr. Stanislav Rosypal, DrSc., Brno, 2006, 290 pp. Díl I. Molekulární biologie prokaryotické buňky. ISBN 80-902562-5-2. URL info
- GLICK, Bernard R. and Jack J. PASTERNAK. Molecular biotechnology :principles and applications of recombinant DNA. 3rd ed. Washington, D.C.: ASM Press, 2003, xxiii, 760. ISBN 1-55581-269-4. info
- Assessment methods
- Lectures are enclosed by test and oral exam.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Autumn 2008, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2008/Bi7430