Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2024
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- doc. RNDr. Sabina Ševčíková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 13 student(s).
Current registration and enrolment status: enrolled: 4/13, only registered: 0/13 - fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course, students should be able to: - design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation - evaluate absolute and relative quantification data
- Learning outcomes
- After attending this class, the students are able to isolate RNA, perform reverse transription as well as real-time PCR including data analysis.
- Syllabus
- Absolute (AQ) and relative (RQ) quantification Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green) Primary data analysis, outputs, statistics MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku. - Teacher's information
- The subject is recommended for students who have no practical experience with qRT-PCR.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2023
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- doc. RNDr. Sabina Ševčíková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 13 student(s).
Current registration and enrolment status: enrolled: 2/13, only registered: 0/13 - fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course, students should be able to: - design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation - evaluate absolute and relative quantification data
- Learning outcomes
- After attending this class, the students are able to isolate RNA, perform reverse transription as well as real-time PCR including data analysis.
- Syllabus
- Absolute (AQ) and relative (RQ) quantification Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green) Primary data analysis, outputs, statistics MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku. - Teacher's information
- The subject is recommended for students who have no practical experience with qRT-PCR.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2022
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- doc. RNDr. Sabina Ševčíková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 13 student(s).
Current registration and enrolment status: enrolled: 0/13, only registered: 0/13 - fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course, students should be able to: - design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation - evaluate absolute and relative quantification data
- Learning outcomes
- After attending this class, the students are able to isolate RNA, perform reverse transription as well as real-time PCR including data analysis.
- Syllabus
- Absolute (AQ) and relative (RQ) quantification Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green) Primary data analysis, outputs, statistics MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku. - Teacher's information
- The subject is recommended for students who have no practical experience with qRT-PCR.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2021
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- doc. RNDr. Sabina Ševčíková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 24. 5. 13:00–17:00 Kontaktujte učitele
- Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 13 student(s).
Current registration and enrolment status: enrolled: 0/13, only registered: 0/13 - fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course, students should be able to: - design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation - evaluate absolute and relative quantification data
- Learning outcomes
- After attending this class, the students are able to isolate RNA, perform reverse transription as well as real-time PCR including data analysis.
- Syllabus
- Absolute (AQ) and relative (RQ) quantification Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green) Primary data analysis, outputs, statistics MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on the extent and intensity of the course: v týdenním bloku. - Teacher's information
- The subject is recommended for students who have no practical experience with qRT-PCR.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2020
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- doc. RNDr. Sabina Ševčíková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 13 student(s).
Current registration and enrolment status: enrolled: 0/13, only registered: 0/13 - fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2019
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Medical Genetics and Molecular Diagnostics (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of Sciencespring 2018
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Medical Genetics and Molecular Diagnostics (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2017
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Medical Genetics and Molecular Diagnostics (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2016
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Medical Genetics and Molecular Diagnostics (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2015
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceSpring 2014
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2012
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi9310c/01: No timetable has been entered into IS.
Bi9310c/02: No timetable has been entered into IS. - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2011
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc. - Timetable of Seminar Groups
- Bi9310c/01: No timetable has been entered into IS. S. Ševčíková
Bi9310c/02: No timetable has been entered into IS. S. Ševčíková - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2010
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Petr Vaňhara, Ph.D. (seminar tutor)
Mgr. Zdeněk Ručka, Ph.D. (assistant)
doc. RNDr. Sabina Ševčíková, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Vaňhara, Ph.D. - Timetable of Seminar Groups
- Bi9310c/1: No timetable has been entered into IS. S. Ševčíková
Bi9310c/2: No timetable has been entered into IS. Z. Ručka
Bi9310c/3: No timetable has been entered into IS. S. Ševčíková
Bi9310c/4: No timetable has been entered into IS. Z. Ručka - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction – practice
Faculty of ScienceSpring 2025
The course is not taught in Spring 2025
- Extent and Intensity
- 0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- doc. RNDr. Sabina Ševčíková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 13 student(s).
Current registration and enrolment status: enrolled: 0/13, only registered: 0/13 - fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- At the end of this course, students should be able to: - design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation - evaluate absolute and relative quantification data
- Learning outcomes
- After attending this class, the students are able to isolate RNA, perform reverse transription as well as real-time PCR including data analysis.
- Syllabus
- Absolute (AQ) and relative (RQ) quantification Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green) Primary data analysis, outputs, statistics MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku. - Teacher's information
- The subject is recommended for students who have no practical experience with qRT-PCR.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2022
The course is not taught in Autumn 2022
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of Scienceautumn 2021
The course is not taught in autumn 2021
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2020
The course is not taught in Autumn 2020
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2019
The course is not taught in Autumn 2019
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2018
The course is not taught in Autumn 2018
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of Scienceautumn 2017
The course is not taught in autumn 2017
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2016
The course is not taught in Autumn 2016
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2015
The course is not taught in Autumn 2015
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2014
The course is not taught in Autumn 2014
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2013
The course is not taught in Autumn 2013
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Sabina Ševčíková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, B-EXB, specialization Antropogenetics)
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2011 - acreditation
The information about the term Autumn 2011 - acreditation is not made public
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Vaňhara, Ph.D. - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
Bi9310c Real-time polymerase chain reaction - practice
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Petr Vaňhara, Ph.D. (seminar tutor)
Mgr. Zdeněk Ručka, Ph.D. (assistant)
doc. RNDr. Sabina Ševčíková, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Vaňhara, Ph.D. - Prerequisites (in Czech)
- ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol ) && NOW( Bi9310 Real-time PCR )
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20 - fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of this course, students should be able to:
- design and correctly perform real-time PCR experiment, including appropriate template, chemistry, and instrumentation manipulation
- evaluate absolute and relative quantification data - Syllabus
- Absolute (AQ) and relative (RQ) quantification
- Template preparation and handling; isolation of total and poly(A)RNA; reverse transcription, RT-PCR (5'nuclease assay, SYBR green)
- Primary data analysis, outputs, statistics
- MIQE standards (Minimum Information for Publication of Quantitative Real-Time PCR Experiments)
- Literature
- Teaching methods
- practical training
- Assessment methods
- active participation, protocol
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: in blocks.
Information on the extent and intensity of the course: v týdenním bloku.
- Enrolment Statistics (recent)