C3002 Biosensors and Nanobiotechnology

Faculty of Science
Spring 2027
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
doc. Mgr. Zdeněk Farka, Ph.D. (lecturer)
Mgr. Jan Přibyl, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Zdeněk Farka, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
Basic knowledge of biochemistry and biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Abstract
Introduction of basic principles of biosensors and nanobiotechnology - applications of nanometer sized biological objects in technology, preparation, characterization and bioconjugation of nanoparticles, and studies of biomolecules, cells and other biological objects with the help of scanning probe microscopies.
Learning outcomes
Student will learn basic principles of biosensors and nanobiotechnology - applications of nanometer sized biological objects in technology, preparation, characterization and bioconjugation of nanoparticles, and studies of biomolecules, cells and other biological objects with the help of scanning probe microscopies.
Key topics
  1. Definition of the biosensor. Historic overview. Characteristics of ideal biosensors.
  2. Electrochemical biosensors, enzyme electrodes. Potentiometric systems and ISFETs. Amperometric measurement of oxygen, hydrogen peroxide and NADH, biosensors based on oxidases and dehydrogenases. Impedimetric and conductometric biosensors.
  3. Spectrophotometric, fluorimetric, and chemiluminescent sensors, optical fibers. Optical biocatalytic sensors. Bioluminescence.
  4. Affinity biosensors based on indirect detection of labels. Immunosensors.
  5. Hybridization biosensors for detecting nucleic acids and oligonucleotide sequences.
  6. Direct optical affinity sensors. Evanescent wave and surface plasmon resonance utilized for monitoring of bioaffinity interactions in real time.
  7. Immobilization of biomolecules for construction of biosensors. Membrane techniques. Electropolymerization. Activation of sensing surfaces. Covalent immobilization of biomolecules.
  8. Miniaturization and mass production of biosensors. Biosensors in integrated analytical microsystems. Biochips. Commercial biosensors.
  9. Nanostructures. Carbon nanotubes, semiconductor nanoparticles - quantum dots. Metal-based nanostructures - nanowires and bioelectronics. Gold nanoparticles (nanorods, nanocages, nanoshells). Magnetic nanoparticles. Separation, characterization and modification of nanoparticles.
  10. Scanning probe microscopy techniques (STM, AFM, SNOM, SECM). Physical principles, basic and advanced measuring modes. Imaging of bioobjects - from atoms and molecules to cells and tissues. Nanolithography and nanomanipulations.
  11. Nanoparticles for biological labeling and cellular imaging. Nanobiosensors and nanobioanalytical systems. Microfluidics, cell sorting and lab-on-a-chip. Biochips and sensing arrays, nanodeposition of biomolecules.
  12. Medical applications. Cytotoxicity of nanoparticles. Nanostructures in drug discovery, delivery, and controlled release. Nanostructures in cancer research. Nanotechnology for tissue engineering and regenerative therapy.
Study resources and literature
  • Nanobiotechnology in diagnosis, drug delivery and treatment. Edited by Mahendra Rai - Mehdi Razzaghi-Abyaneh - Avinash P. Ingle. First published. Hoboken, NJ: Wiley-Blackwell, 2021, xii, 404. ISBN 9781119671770. info
  • Kaushik_Nanobiotechnology_for_sensing_applications_from_lab_to_field_2017
  • Tomar_Nanobiotechnology_Concepts_and_Applications_in_Health_Agriculture_and_Environment_2020
Approaches, practices, and methods used in teaching
Lectures with study materials provided in the Information System.
Method of verifying learning outcomes and course completion requirements

Written exam. Scoring above 50% of points is required for the successful completion of the exam.

Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught every week.
The course is also listed under the following terms Spring 2022, Spring 2023, Spring 2024, Spring 2025, Spring 2026.
  • Enrolment Statistics (Spring 2027, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2027/C3002