C7998 Basics of Experimental NMR Spectroscopy

Faculty of Science
Autumn 2015
Extent and Intensity
0/0/1. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Lukáš Maier, Ph.D. (seminar tutor)
Mgr. Zdeněk Moravec, Ph.D. (seminar tutor)
prof. RNDr. Radek Marek, Ph.D. (alternate examiner)
Guaranteed by
prof. RNDr. Radek Marek, Ph.D.
Department of Chemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Chemistry - Chemistry Section - Faculty of Science
Prerequisites
C1020 General Chemistry
Basic knowledge of NMR spectroscopy.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
Students will learn basics of 1D NMR measurement. Students will be able to prepare NMR sample, measure NMR spectra and process data of common nuclei : 1H, 13C, 19F, 11B, 31P at the end of the course.
Syllabus
  • NMR lab introducing

  • AVANCE 300 MHz NMR spectrometer: construction of cryomagnet
  • NMR probes
  • Temperature unit
  • Hardware and software (TopSpin)
  • Basics of pulse-field NMR spectrometry:

  • deuterium stabilization (lock)
  • tunning of field homogenity (shimming)
  • acquisition parameters, pulse sequences, FID (Free induction decay)
  • matemathical operation with FIDs – windows functions Fourier transformation, data processing, phase corrections
  • Measurement and interpretation of 1H NMR spectra

  • Measurement of 1H NMR (300 MHz) spectra of unknown compound CDCl3 at 298 and 313 K under operator guidance
  • 1H NMR spectra interpretation: o integral intensity, spin-spin couplings, chemical Shift determinativ and overall structural elucidation
  • Measurement and interpretation of 13C NMR spectra (APT), 11B and 19F NMR or other nuclei on regest

  • Measurement of 13C NMR (75MHz) spectra with and withou proton decoupling under operator guidance
  • APT spectra
  • Interpretation of 13C and APT NMR spectra
  • Measurement and interpretation of 11B and 19F NMR spectra of unknown compound.
Literature
    recommended literature
  • Claridge, Timothy D.W. High-Resolution NMR Techniques in Organic Chemistry, Amsterdam, Pergamon, 1999, ISBN 0-08-042798-7
  • Braun, S. - Kalinowski, H.O. - Berger, S. 100 and More Basic NMR Experiments, Weinheim, VCH, 1996, ISBN 3-527-29091-5
Teaching methods
Practical training
Assessment methods
Full attandance is mandatory. Student has to performed basic NMR experiments (1H, 13C and APT).
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught each semester.
The course is taught: in blocks.
Teacher's information
http://nmrlab.chemi.muni.cz/c7998.php
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Spring 2007, Autumn 2007, Spring 2008, Autumn 2008, Spring 2009, Autumn 2009, Spring 2010, Autumn 2010, Spring 2011, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Spring 2016, Autumn 2016, Spring 2017, autumn 2017, spring 2018, Autumn 2018, Spring 2019, Autumn 2019, Spring 2020.
  • Enrolment Statistics (Autumn 2015, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2015/C7998