PřF:Bi7340 Cytol. and morphol. of bact. - Course Information
Bi7340 Cytology and morphology of bacteria
Faculty of ScienceAutumn 2015
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Gabriela Rotková, Ph.D. (lecturer)
Mgr. Jana Čmielová, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Gabriela Rotková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Thu 14:00–15:50 E25/111
- Prerequisites
- NOW( Bi7330 Cytology and morphology of bacteria )
general microbiology - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 25 student(s).
Current registration and enrolment status: enrolled: 0/25, only registered: 0/25, only registered with preference (fields directly associated with the programme): 0/25 - fields of study / plans the course is directly associated with
- there are 6 fields of study the course is directly associated with, display
- Course objectives
- Students will be able consider the sutiable technique for observing microscopic and macroscopic characteristics of bacteria and their morphological diversity (including the cell morphology of particular bacterial species depending on cultivating media) and observing structures of bacterial cells (endospores, inclusions, flagellum). Student will be able consider the applicability of particular methods for clinical, environmental and biotechnological practice. Student will be able of preparing of variable stained and native microscopic slides, will be able operate variable microscopical techniques (phase and Nomarsky contrast, bright field microscopy) and handle with images acquired by CCD camera, photographic camera and analyse them (image acquisition - LUCIA G).
- Syllabus
- Morphology of bacterial and archaeal cells and colonies, basic staining methods.
- Techniques of work with light microscope, care, removing of simple problems.
- Microphotography, digital photography, the use of video-cameras in image acquisition in microbiology, computer image analysis.
- Determination of the size of bacterial cells and their morphological structures (the use of ocular micrometer and software for image analysis).
- Gram staining. Acid-fast bacteria, Ziehl-Neelsen staining, mycobacteria. Giemsa staining.
- Endospores and sporulation of bacteria.
- Phase contrast and Nomarski differential interference contrast.
- Capsules, sheaths and slimes of bacterial cells.
- Movement of bacterial cells.
- Technique of slide cultures.
- Actinomycetes and methods of their morphology observation.
- Measurement and comparison of proportions of selected groups of microorganisms.
- Staining of cell wall, nuclear material and reserved compounds in cells of bacteria and yeasts.
- Literature
- PERRY, Jerome J. and James T. STALEY. Microbiology :dynamics and diversity. Fort Worth: Saunders College Publishing, 1997, xxxi, 911. ISBN 0-03-053893-9. info
- Laboratory experiments in microbiology. Edited by Ted R. Johnson - Christine L. Case. 4th ed. Redwood City: Benjamin Cummings, 1995, xi, 418 s. ISBN 0-8053-8509-6. info
- Teaching methods
- laboratory course, outcomes discussion, electronical protocol
- Assessment methods
- practical documentation of laboratory courses, individual preparing of electronical protocols, discussion about individual results, written exam, examination preparations
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
- Enrolment Statistics (Autumn 2015, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2015/Bi7340