PřF:Bi7270c Plant Embryology - Practice - Course Information
Bi7270c Plant Embryology - Practice
Faculty of ScienceAutumn 2023
- Extent and Intensity
- 0/3/0. 3 credit(s). Type of Completion: z (credit).
- Teacher(s)
- Mgr. Hana Cempírková, Ph.D. (seminar tutor)
- Guaranteed by
- Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 7:00–9:50 D36/209
- Prerequisites (in Czech)
- NOW ( Bi7270 Plant Embryology )
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cell Biology (programme PřF, B-EMB)
- Experimental Plant Biology (programme PřF, N-EBR)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Special Biology (programme PřF, N-EXB)
- Special Biology (programme PřF, N-EXB, specialization Experimentální biologie rostlin)
- Course objectives
- At the end of this course, students should be able to:
describe the development of angiosperm reproductive organs;
understand the processes of pollen-pistil interactions;
explain the process of double fertilization in angiosperms and to interpret seed development. Regulation of above mentioned processes will be illustrated on selected plant models. - Learning outcomes
- At the end of this course, students should be able to:
describe the development of angiosperm reproductive organs;
understand the processes of pollen-pistil interactions;
explain the process of double fertilization in angiosperms and to interpret seed development. Regulation of above mentioned processes will be illustrated on selected plant models. - Syllabus
- Seed as a start of a new life cycle: angiosperm seed structure (testa, endosperm, aleuron layer, embryo: embryonal axis, cotyledones, hypocotyl), seed quiescence and dormancy, seed germination, seedling structure.
- Mitosis - nuclear division: Cell cycle, cytoskeleton formation during cell cycle, polarity, asymmetric divisions, squash methods, pretreatments for karyology, cell cycle synchronization.
- Morphology of the vegetative stem apical meristem (structure, stem cells, leaf primordia and leaf meristems, leaf differentiation); root apical meristem (quiescent centre, root cap, columela).
- Megagametogenesis, gynaeceum, pistil, angiosperm ovule structure, clearing methods of plant ovules, observation of cleared ovules by means of Nomarski DIC.
- Microsporogenesis and microgametogenesis, androeceum, stamen: anther and filamentum, structure of the anther wall, tetrades, types of pollen grains, differential staining of pollen grains, determination of pollen viability.
- Pollen grain germination in vitro, phase contrast microscopy.
- Pollen - pistil interaction: pollen tube growth, callose plugs, decolorised aniline blue staining, fluorescence microscopy in UV light.
- Cryostat sections in embryology - fixation, cryoprotection, cryo-sectioning (cryostat), enzyme histochemistry, histochemical reactions of hydrolytic enzymes.
- Histological techniques in plant embryology I. - fixation of isolated organs.
- Histological techniques in plant embryology II. Dehydration of samples, paraffin and resin embedding, microtome sectioning, adhesion of sections, de-waxing of sections.
- Histological techniques in plant embryology III. Successive double staining of sections, dehydration and Eukitt mounting.
- Documentation of prepared microscopic preparations - drawings, measurements, micrographs, automatic analysis of images.
- Literature
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
- FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
- KIERNAN, J. A. Histological & histochemical methods : theory and practice. 3rd ed. London: Arnold, 2003, x, 502. ISBN 0750649364. info
- Teaching methods
- Laboratory course.
- Assessment methods
- Group projects, elaboration of protocols.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
- Enrolment Statistics (Autumn 2023, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2023/Bi7270c