PřF:Bi2302 Zoological microtechnique - Course Information
Bi2302 Zoological microtechnique
Faculty of ScienceAutumn 2022
- Extent and Intensity
- 0/2/0. 3 credit(s). Type of Completion: k (colloquium).
- Teacher(s)
- Mgr. Mária Seifertová, Ph.D. (seminar tutor)
- Guaranteed by
- Mgr. Mária Seifertová, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Mgr. Mária Seifertová, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi2302/01: Mon 10:00–11:50 D36/223, M. Seifertová
Bi2302/02: Mon 14:00–15:50 D36/223, M. Seifertová
Bi2302/03: Mon 16:00–17:50 D36/223, M. Seifertová
Bi2302/04: Tue 12:00–13:50 D36/223, M. Seifertová - Prerequisites
- (! Bi1050 Biological techniques ) && (!NOW( Bi1050 Biological techniques )) && (! Bi1301 Microscopic Techniques ) && (!NOW( Bi1301 Microscopic Techniques ))
(!Bi1050) && (!NOW(Bi1050)) - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Ecological and Evolutionary Biology (programme PřF, B-EKB)
- Experimental Animal Biology and Immunology (programme PřF, B-EMB)
- Special Biology (programme PřF, B-EXB)
- Special Biology (programme PřF, B-EXB, specialization Experimental Biology of Animals and Immunology)
- Course objectives
- The main purpose in the course is to acquaint students with the basic techniques of light microscopy and the possibilities of processing biological material in the form of different types of prepared zoological microscope slides.
- Learning outcomes
- At the end of this course, students should be able to: work with light microscope; effectively use the specialized techniques of light microscopy (dark field, phase contrast, differential interference contrast, fluorescence, drawing, measuring); prepare basic types of microscope slides (fixation, staining, mounting in the media, histological slides) and propose a suitable method of processing biological material in the form of a prepared microscope slides.
- Syllabus
- Light microscopy - dark field, phase contrast, differential interference contrast, fluorescence, measurement methods, drawing. Basic zoological techniques - native slides, vital staining (neutral red, Methylene green); Permanent slides, framing, fixation, staining; glycerol, glycerol jelly, Liquido-Faure; resinous media - canada balsam, Entellan. Histological slides - embedding, cutting and staining.
- Literature
- Handbook of biological confocal microscopy. Edited by James B. Pawley. 3rd ed. New York: Springer, 2006, xxviii, 98. ISBN 038725921X. info
- MURPHY, Douglas B. Fundamentals of light microscopy and electronic imaging. New York: Wiley-Liss, 2001, xii, 368 s. ISBN 0-471-25391-X. info
- KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
- Teaching methods
- laboratory practice
- Assessment methods
- Lectures and laboratory practice, colloquium
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught each semester.
Information on the per-term frequency of the course: Předmět je ve stejné podobě vyučován v podzimním i jarním semestru. - Listed among pre-requisites of other courses
- Bi1050 Biological techniques
!Bi2302 && !Bi1301 && (!NOW(Bi2302)) && (!NOW(Bi1301)) - Bi4170 Optical contrast and imaging methods
Bi1050||Bi2302||Bi1301
- Bi1050 Biological techniques
- Enrolment Statistics (Autumn 2022, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2022/Bi2302