The seasonal changes of innate immunity of tench, Tinca tinca
(L.) with different ploidy level

a 2012

The seasonal changes of innate immunity of tench, Tinca tinca (L.) with different ploidy level

VOJTEK, Libor, Soňa TOLAROVÁ, Martina DÁVIDOVÁ, Andrea VETEŠNÍKOVÁ ŠIMKOVÁ, Martin FLAJŠHANS et. al.

Basic information

Original name

The seasonal changes of innate immunity of tench, Tinca tinca (L.) with different ploidy level

Authors

VOJTEK, Libor (203 Czech Republic, belonging to the institution), Soňa TOLAROVÁ (203 Czech Republic, belonging to the institution), Martina DÁVIDOVÁ (203 Czech Republic, belonging to the institution), Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (703 Slovakia, belonging to the institution), Martin FLAJŠHANS (203 Czech Republic) and Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution)

Edition

VIth International Workshop on Biology and Culture of the Tench (Tinca tinca L.), 2012

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30102 Immunology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/12:00057615

Organization unit

Faculty of Science

Keywords (in Czech)

tench; innate immunity; seasonal changes; ploidy level; 11-ketotestosterone

Keywords in English

tench; innate immunity; seasonal changes; ploidy level; 11-ketotestosterone

Abstract

V originále

The aim of this study was to analyze the effect of fish ploidy level and seasonality on innate immune response of tench (Tinca tinca, Cyprinidae), fish species extensively bred in European aquaculture. Fish samples were collected from breeding pond in Vodňany (Czech Republic) during four sampling periods (September 2008, December 2008, March 2009 and June 2009). A total of 81 diploid and 79 triploid specimens of tench were investigated. All hematological parameters measured (erythrocyte count, hematocrit, hemoglobin concentration and leukocyte count) were strongly affected by the season and fish ploidy status. The effect of fish sex was found only in leukocyte count. Blood innate immunity measured by respiratory burst as activity of phagocytes and complement activity were significantly different among different sampling periods (e.g. phagocytes showed lowest activity in winter whereas complement activity was the highest in this period). Moreover, respiratory burst was also influenced by fish ploidy (with lower values in diploids comparing to triploids). Lysozyme activity (higher in autumn and spring) in skin mucus showed significant seasonal changes. However, ploidy had no significant effect on lysozyme. No effect of sex on innate immunity was observed. The level of stress measured by glucose concentration and the concentration of 11-ketotestosterone considered as the steroid hormone affecting immunity only in males showed only seasonal changes and no effect of ploidy was found. This work was supported by the project of Grant Agency of Czech Republic Project. No. 524/07/0188 and partly also by projects CENAKVA CZ.1.05/2.1.00/01.0024 and GAJU 047/2010/Z (Martin Flajšhans). A. Šimková was funded by the Czech Science Foundation, Project No. P505/12/G112 (ECIP).

Links

GA524/07/0188, research and development project

Name: Úloha imunitní investice v kontextu kompromisů: imunoekologické studium vztahů mezi reprodukcí, imunitou a parazitizmem u sladkovodních ryb

Investor: Czech Science Foundation, The role of immune investment in the context of trade-offs: immuno-ecological study of the relationships among reproduction, immunity and parasitism

Citovat

VOJTEK, Libor, Soňa TOLAROVÁ, Martina DÁVIDOVÁ, Andrea VETEŠNÍKOVÁ ŠIMKOVÁ, Martin FLAJŠHANS and Pavel HYRŠL. The seasonal changes of innate immunity of tench, Tinca tinca (L.) with different ploidy level. In VIth International Workshop on Biology and Culture of the Tench (Tinca tinca L.). 2012.

@proceedings{1066733,
   author = {Vojtek, Libor and Tolarová, Soňa and Dávidová, Martina and Vetešníková Šimková, Andrea and Flajšhans, Martin and Hyršl, Pavel},
   booktitle = {VIth International Workshop on Biology and Culture of the Tench (Tinca tinca L.)},
   keywords = {tench; innate immunity; seasonal changes; ploidy level; 11-ketotestosterone},
   language = {eng},
   title = {The seasonal changes of innate immunity of tench, Tinca tinca (L.) with different ploidy level},
   year = {2012}
}

TY  - CONF
ID  - 1066733
AU  - Vojtek, Libor - Tolarová, Soňa - Dávidová, Martina - Vetešníková Šimková, Andrea - Flajšhans, Martin - Hyršl, Pavel
PY  - 2012
TI  - The seasonal changes of innate immunity of tench, Tinca tinca (L.) with different ploidy level
KW  - tench
KW  - innate immunity
KW  - seasonal changes
KW  - ploidy level
KW  - 11-ketotestosterone
N2  - The aim of this study was to analyze the effect of fish ploidy level and seasonality on innate immune response of tench (Tinca tinca, Cyprinidae), fish species extensively bred in European aquaculture. Fish samples were collected from breeding pond in Vodňany (Czech Republic) during four sampling periods (September 2008, December 2008, March 2009 and June 2009). A total of 81 diploid and 79 triploid specimens of tench were investigated. All hematological parameters measured (erythrocyte count, hematocrit, hemoglobin concentration and leukocyte count) were strongly affected by the season and fish ploidy status. The effect of fish sex was found only in leukocyte count. Blood innate immunity measured by respiratory burst as activity of phagocytes and complement activity were significantly different among different sampling periods (e.g. phagocytes showed lowest activity in winter whereas complement activity was the highest in this period). Moreover, respiratory burst was also influenced by fish ploidy (with lower values in diploids comparing to triploids). Lysozyme activity (higher in autumn and spring) in skin mucus showed significant seasonal changes. However, ploidy had no significant effect on lysozyme. No effect of sex on innate immunity was observed. The level of stress measured by glucose concentration and the concentration of 11-ketotestosterone considered as the steroid hormone affecting immunity only in males showed only seasonal changes and no effect of ploidy was found. This work was supported by the project of Grant Agency of Czech Republic Project. No. 524/07/0188 and partly also by projects CENAKVA CZ.1.05/2.1.00/01.0024 and GAJU 047/2010/Z (Martin Flajšhans). A. Šimková was funded by the Czech Science Foundation, Project No. P505/12/G112 (ECIP).
ER  -

VOJTEK, Libor, Soňa TOLAROVÁ, Martina DÁVIDOVÁ, Andrea VETEŠNÍKOVÁ ŠIMKOVÁ, Martin FLAJŠHANS and Pavel HYRŠL. The seasonal changes of innate immunity of tench, Tinca tinca (L.) with different ploidy level. In \textit{VIth International Workshop on Biology and Culture of the Tench (Tinca tinca L.)}. 2012.

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