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@article{1066896,
author = {Nováček, Jiří and Haba, Noam Y and Chill, Jordan H and Žídek, Lukáš and Sklenář, Vladimír},
article_location = {Dordrecht},
article_number = {2},
doi = {http://dx.doi.org/10.1007/s10858-012-9631-8},
keywords = {Intrinsically disordered proteins; Non-uniform sampling; C-13 detection; Chemical shifts; Residual secondary structure; Prolines assignment},
language = {eng},
issn = {0925-2738},
journal = {Journal of Biomolecular NMR},
title = {4D Non-uniformly sampled HCBCACON and (1) J(NC alpha)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins},
url = {http://www.springerlink.com/content/g5116115mh441831/?MUD=MP},
volume = {53},
year = {2012}
}
TY - JOUR
ID - 1066896
AU - Nováček, Jiří - Haba, Noam Y - Chill, Jordan H - Žídek, Lukáš - Sklenář, Vladimír
PY - 2012
TI - 4D Non-uniformly sampled HCBCACON and (1) J(NC alpha)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins
JF - Journal of Biomolecular NMR
VL - 53
IS - 2
SP - 139-148
EP - 139-148
PB - Springer Netherlands
SN - 09252738
KW - Intrinsically disordered proteins
KW - Non-uniform sampling
KW - C-13 detection
KW - Chemical shifts
KW - Residual secondary structure
KW - Prolines assignment
UR - http://www.springerlink.com/content/g5116115mh441831/?MUD=MP
L2 - http://www.springerlink.com/content/g5116115mh441831/?MUD=MP
N2 - A pair of 4D NMR experiments for the backbone assignment of disordered proteins is presented. The experiments exploit C-13 direct detection and non-uniform sampling of the indirectly detected dimensions, and provide correlations of the aliphatic proton (H-alpha, and H-beta) and carbon (C-alpha, C-beta) resonance frequencies to the protein backbone. Thus, all the chemical shifts regularly used to map the transient secondary structure motifs in the intrinsically disordered proteins (H-alpha, C-alpha, C-beta, C', and N) can be extracted from each spectrum. Compared to the commonly used assignment strategy based on matching the C-alpha and C-beta chemical shifts, inclusion of the H-alpha and H-beta provides up to three extra resonance frequencies that decrease the chance of ambiguous assignment. The experiments were successfully applied to the original assignment of a 12.8 kDa intrinsically disordered protein having a high content of proline residues (26 %) in the sequence.
ER -
NOVÁČEK, Jiří, Noam Y HABA, Jordan H CHILL, Lukáš ŽÍDEK a Vladimír SKLENÁŘ. 4D Non-uniformly sampled HCBCACON and (1) J(NC alpha)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins. \textit{Journal of Biomolecular NMR}. Dordrecht: Springer Netherlands, 2012, roč.~53, č.~2, s.~139-148. ISSN~0925-2738. Dostupné z: https://dx.doi.org/10.1007/s10858-012-9631-8.
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