Intrathecal Administration of FluoroRuby and its Relation to
the Neuroinflammatory Reaction in the Rat Spinal Nerve Roots of
Neuropathic Pain Model

a 2012

Intrathecal Administration of FluoroRuby and its Relation to the Neuroinflammatory Reaction in the Rat Spinal Nerve Roots of Neuropathic Pain Model

JOUKAL, Marek, Matej HALAJ, Ilona KLUSÁKOVÁ and Petr DUBOVÝ

Basic information

Original name

Intrathecal Administration of FluoroRuby and its Relation to the Neuroinflammatory Reaction in the Rat Spinal Nerve Roots of Neuropathic Pain Model

Name in Czech

Intrathékální aplikace FluoroRuby a jeho vztah k neurozánětové reakci ve spinálních kořenech laboratorního potkana při modelu neuropatické bolesti

Authors

JOUKAL, Marek, Matej HALAJ, Ilona KLUSÁKOVÁ and Petr DUBOVÝ

Edition

Morphology 2012, 2012

Other information

Type of outcome

Konferenční abstrakt

Field of Study

30000 3. Medical and Health Sciences

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Medicine

Keywords (in Czech)

neuropatická bolest, FluoroRuby, intrathální aplikace

Keywords in English

neuropathic pain, FluoroRuby, intrathecal administration

Změněno: 5/2/2013 16:02, MUDr. Ilona Klusáková, Ph.D.

Abstract

V originále

There is an evidence that demyelinization occurs in the rat spinal nerve roots (SNR) of neuropathic pain model. It gives the background for hypothetic diffusion of neuroinflammatory mediators from liquor to the SNR. In our experiments we used intrathecally administered FluoroRuby (FR) to trace penetration of molecules from subarachnoid space to SNR. Twelve adult male rats (Wistar, 200-250 g) were divided into chronic constriction injury group (Group A) (n=7), sham-operated group (Group B) (n=2) and intact group (Group C) (n=3). For surgery, animals were deeply anesthetized and three ligatures were applied on left sciatic nerve (group A). After 3 days, FR was applicated into subarachnoid space in lumbar segments and the animals were left to survive for 18-20 hours. In sham operated rats the left sciatic nerve was only separated (Group B). After 3 days, the animals were reanesthetized and FR was applied into subarachnoid space at the level of lumbar segments, then rats were left to survive for 24 hours. Intact rats (group C) were deeply anesthetized and FR was intrathecally applied and rats were left to survive for 24 hours. Then, the animals were deeply anesthetized with a lethal dose of sodium pentobarbital, perfused transcardially and the SNR connected with dorsal root ganglia of lumbar (L4-L5) and cervical (C7-C8) segments were removed and fixed overnight. The distribution of FR was observed in fluorescent microscope. The strong red fluorescence in dorsal and ventral spinal nerve root indicated diffusion of FR from liquor into the SNR. The particles of dextran were found in both lumbar and cervical SNR of each group. In addition, the ipsilateral lumbal SNR of group A displayed the FR loaded cells which can be connected with neuroinflammatory reaction in SNR.

Links

MUNI/A/0907/2011, interní kód MU

Name: Projevy neurozánětu ve spinálních kořenech po poškození periferního nervu

Investor: Masaryk University, Category A

Citovat

JOUKAL, Marek, Matej HALAJ, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Intrathecal Administration of FluoroRuby and its Relation to the Neuroinflammatory Reaction in the Rat Spinal Nerve Roots of Neuropathic Pain Model. In Morphology 2012. 2012.

@proceedings{1066955,
   author = {Joukal, Marek and Halaj, Matej and Klusáková, Ilona and Dubový, Petr},
   booktitle = {Morphology 2012},
   keywords = {neuropathic pain, FluoroRuby, intrathecal administration},
   title = {Intrathecal Administration of FluoroRuby and its Relation to the Neuroinflammatory Reaction in the Rat Spinal Nerve Roots of Neuropathic Pain Model},
   url = {http://cas.lf1.cuni.cz/sjezd/program.pdf},
   year = {2012}
}

TY  - CONF
ID  - 1066955
AU  - Joukal, Marek - Halaj, Matej - Klusáková, Ilona - Dubový, Petr
PY  - 2012
TI  - Intrathecal Administration of FluoroRuby and its Relation to the Neuroinflammatory Reaction in the Rat Spinal Nerve Roots of Neuropathic Pain Model
KW  - neuropathic pain, FluoroRuby, intrathecal administration
UR  - http://cas.lf1.cuni.cz/sjezd/program.pdf
N2  - There is an evidence that demyelinization occurs in the rat spinal nerve roots (SNR) of neuropathic pain model. It gives the background for hypothetic diffusion of neuroinflammatory mediators from liquor to the SNR. In our experiments we used intrathecally administered FluoroRuby (FR) to trace penetration of molecules from subarachnoid space to SNR. Twelve adult male rats (Wistar, 200-250 g) were divided into chronic constriction injury group (Group A) (n=7), sham-operated group (Group B) (n=2) and intact group (Group C) (n=3). For surgery, animals were deeply anesthetized and three ligatures were applied on left sciatic nerve (group A). After 3 days, FR was applicated into subarachnoid space in lumbar segments and the animals were left to survive for 18-20 hours. In sham operated rats the left sciatic nerve was only separated (Group B). After 3 days, the animals were reanesthetized and FR was applied into subarachnoid space at the level of lumbar segments, then rats were left to survive for 24 hours. Intact rats (group C) were deeply anesthetized and FR was intrathecally applied and rats were left to survive for 24 hours. Then, the animals were deeply anesthetized with a lethal dose of sodium pentobarbital, perfused transcardially and the SNR connected with dorsal root ganglia of lumbar (L4-L5) and cervical (C7-C8) segments were removed and fixed overnight. The distribution of FR was observed in fluorescent microscope. The strong red fluorescence in dorsal and ventral spinal nerve root indicated diffusion of FR from liquor into the SNR. The particles of dextran were found in both lumbar and cervical SNR of each group. In addition, the ipsilateral lumbal SNR of group A displayed the FR loaded cells which can be connected with neuroinflammatory reaction in SNR.
ER  -

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