| SEDLÁČEK, Ivo, Eva KREJČÍ, Anna ANDĚLOVÁ, Michaela SEDLÁČKOVÁ, Iva PORAZILOVÁ a Pavla HOLOCHOVÁ. Aeromonas hydrophila subsp. dhakensis - a causative agent of gastroenteritis imported into the Czech Republic. Annals of Agricultural and Environmental Medicine. 2012, roč. 19, č. 3, s. 409-413. ISSN 1232-1966. |
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@article{1067698,
author = {Sedláček, Ivo and Krejčí, Eva and Andělová, Anna and Sedláčková, Michaela and Porazilová, Iva and Holochová, Pavla},
article_number = {3},
keywords = {Aeromonas; identification; ribotyping; fatty acids; cpn60 sequencing},
language = {eng},
issn = {1232-1966},
journal = {Annals of Agricultural and Environmental Medicine},
title = {Aeromonas hydrophila subsp. dhakensis - a causative agent of gastroenteritis imported into the Czech Republic},
volume = {19},
year = {2012}
}
TY - JOUR
ID - 1067698
AU - Sedláček, Ivo - Krejčí, Eva - Andělová, Anna - Sedláčková, Michaela - Porazilová, Iva - Holochová, Pavla
PY - 2012
TI - Aeromonas hydrophila subsp. dhakensis - a causative agent of gastroenteritis imported into the Czech Republic
JF - Annals of Agricultural and Environmental Medicine
VL - 19
IS - 3
SP - 409-413
EP - 409-413
SN - 12321966
KW - Aeromonas
KW - identification
KW - ribotyping
KW - fatty acids
KW - cpn60 sequencing
N2 - Out of the twenty-one A. hydrophila complex isolates obtained during a routine examination of human diarrhoeal faeces, two A. hydrophila subsp. dhakensis isolates (P1097 = CCM 7329 and P1165) were successfully identified by ribotyping. The correct taxonomic position of the A. hydrophila subsp. dhakensis CCM 7329 was verified by cpn60 sequencing (GeneBank accession number HM536193). The remaining A. hydrophila complex isolates were identified as A. hydrophila subsp. hydrophila. The ability of biochemical tests and fatty acid methyl ester analysis to reliably discern both A. hydrophila subsp. dhakensis and A. hydrophila subsp. hydrophila was limited. In contrast to the A. hydrophila subsp. hydrophila, the faecal isolates of A. hydrophila subsp. dhakensis did not produce acid from arbutin. When compared in a two-dimensional plot, the A. hydrophila subsp. dhakensis faecal isolates contained higher amounts of the two minor fatty acids than the A. hydrophila subsp. hydrophila reference strain. This is the first detected occurrence of the less frequent A. hydrophila subsp. dhakensis in our region and ribotyping was proved as a suitable method for the identification of A. hydrophila subsp. dhakensis.
ER -
SEDLÁČEK, Ivo, Eva KREJČÍ, Anna ANDĚLOVÁ, Michaela SEDLÁČKOVÁ, Iva PORAZILOVÁ a Pavla HOLOCHOVÁ. Aeromonas hydrophila subsp. dhakensis - a causative agent of gastroenteritis imported into the Czech Republic. \textit{Annals of Agricultural and Environmental Medicine}. 2012, roč.~19, č.~3, s.~409-413. ISSN~1232-1966.
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