Electrochamical characterization of doxorubicin interaction
with DNA

a 2012

Electrochamical characterization of doxorubicin interaction with DNA

MASAŘÍK, Michal, Ludmila KREJČOVÁ, Vojtěch ADAM, David HYNEK, Marie STIBOROVÁ et. al.

Základní údaje

Originální název

Electrochamical characterization of doxorubicin interaction with DNA

Autoři

MASAŘÍK, Michal, Ludmila KREJČOVÁ, Vojtěch ADAM, David HYNEK, Marie STIBOROVÁ, Tomáš ECKSCHLAGER a René KIZEK

Vydání

15th International Symposium on Molecular Medicine, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10405 Electrochemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.957

Organizační jednotka

Lékařská fakulta

ISSN

UT WoS

000310651600165

Klíčová slova česky

doxorubicin; electrochemistry; DNA interaction

Změněno: 17. 10. 2012 12:51, Mgr. Martina Raudenská, Ph.D.

Anotace

V originále

Doxorubicin as a member of anthracycline antibiotics is one of the most widely used for the treatment of malignant tumours with the ability to i11teract with DNA. Electrochemistry is considered one of the most sensitive methods for studying DNA. The aim of the study was: i) electrochemical characterization of the interaction of doxorubicin with single and double stranded oligonucleotides. ii) investigation of the influence of different sequence on the interaction. Hi) time dependence of the effect of doxorubicin and oligonucleotides. Four different variants of ODN were designed and the influence of sequence on interaction with ctoxorubicin was investigated. The obtained data were processed showing the correlation dependence of size change of DOXO peak to peak size change of the peak of each ODN. This dependence was studied also in the time manner (time interaction DOXO with ODN) as 30, 150, 240 and 300 min. The value of (100,0) represents the initial state of electrochemical analysis. This puint must be understood as a starting point for all four of the oligonucleotides (MT5, GL6, GIA and CA3). When comparing the results for three ratios of the mixture between drug and ssODN and/or dsODN we found that the interval of peak intensities of oligonucleotide reduced with the increasing ratio of oligonucleotide doxorubicin, i.e. for ratio 1:1 it is 25-85 %, for ratio 1:2 it is 16-60 % and for ratio 1:5 it is 19-51 %. The interval of relative signal intensities of doxorubicin ranges from 8 to 100 % for ratio 1:1, 22-100 % for ratios 1:2 and 1:5. The results showed the assumption that DOXO peak height increased with increasing concentration of the drug intercalated into ssODN and dsODN.

Citovat

MASAŘÍK, Michal, Ludmila KREJČOVÁ, Vojtěch ADAM, David HYNEK, Marie STIBOROVÁ, Tomáš ECKSCHLAGER a René KIZEK. Electrochamical characterization of doxorubicin interaction with DNA. In 15th International Symposium on Molecular Medicine. 2012. ISSN 1107-3756.

@proceedings{1068960,
   author = {Masařík, Michal and Krejčová, Ludmila and Adam, Vojtěch and Hynek, David and Stiborová, Marie and Eckschlager, Tomáš and Kizek, René},
   booktitle = {15th International Symposium on Molecular Medicine},
   language = {eng},
   title = {Electrochamical characterization of doxorubicin interaction with DNA},
   year = {2012}
}

TY  - CONF
ID  - 1068960
AU  - Masařík, Michal - Krejčová, Ludmila - Adam, Vojtěch - Hynek, David - Stiborová, Marie - Eckschlager, Tomáš - Kizek, René
PY  - 2012
TI  - Electrochamical characterization of doxorubicin interaction with DNA
N2  - Doxorubicin as a member of anthracycline antibiotics is one of the most widely used for the treatment of malignant tumours with the ability to i11teract with DNA. Electrochemistry is considered one of the most sensitive methods for studying DNA. The aim of the study was: i) electrochemical characterization of the interaction of doxorubicin with single and double stranded oligonucleotides. ii) investigation of the influence of different sequence on the interaction. Hi) time dependence of the effect of doxorubicin and oligonucleotides. Four different variants of ODN were designed and the influence of sequence on interaction with ctoxorubicin was investigated. The obtained data were processed showing the correlation dependence of size change of DOXO peak to peak size change of the peak of each ODN. This dependence was studied also in the time manner (time interaction DOXO with ODN) as 30, 150, 240 and 300 min. The value of (100,0) represents the initial state of electrochemical analysis. This puint must be understood as a starting point for all four of the oligonucleotides (MT5, GL6, GIA and CA3). When comparing the results for three ratios of the mixture between drug and ssODN and/or dsODN we found that the interval of peak intensities of oligonucleotide reduced with the increasing ratio of oligonucleotide doxorubicin, i.e. for ratio 1:1 it is 25-85 %, for ratio 1:2 it is 16-60 % and for ratio 1:5 it is 19-51 %. The interval of relative signal intensities of doxorubicin ranges from 8 to 100 % for ratio 1:1, 22-100 % for ratios 1:2 and 1:5. The results showed the assumption that DOXO peak height increased with increasing concentration of the drug intercalated into ssODN and dsODN.
ER  -

MASAŘÍK, Michal, Ludmila KREJČOVÁ, Vojtěch ADAM, David HYNEK, Marie STIBOROVÁ, Tomáš ECKSCHLAGER a René KIZEK. Electrochamical characterization of doxorubicin interaction with DNA. In \textit{15th International Symposium on Molecular Medicine}. 2012. ISSN~1107-3756.

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