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@article{1070079, author = {Niemi, Maarit R. and Ollinkangas, Tuula and Paulin, Lars and Švec, Pavel and Vandamme, Peter and Karkman, Antti and Kosina, Marcel and Lindstrom, Kristina}, article_location = {UK}, article_number = {9}, doi = {http://dx.doi.org/10.1099/ijs.0.038257-0}, keywords = {Enterococcus rivorum sp. nov.; pristine brooks; taxonomy}, language = {eng}, issn = {1466-5026}, journal = {International Journal of Systematic and Evolutionary Microbiology}, title = {Enterococcus rivorum sp. nov., from water of pristine brooks}, volume = {62}, year = {2012} }
TY - JOUR ID - 1070079 AU - Niemi, Maarit R. - Ollinkangas, Tuula - Paulin, Lars - Švec, Pavel - Vandamme, Peter - Karkman, Antti - Kosina, Marcel - Lindstrom, Kristina PY - 2012 TI - Enterococcus rivorum sp. nov., from water of pristine brooks JF - International Journal of Systematic and Evolutionary Microbiology VL - 62 IS - 9 SP - 2169-2173 EP - 2169-2173 PB - Society for General Microbiology SN - 14665026 KW - Enterococcus rivorum sp. nov. KW - pristine brooks KW - taxonomy N2 - A significant number of Enterococcus strains from pristine waters of two brooks in Finland formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional SDS-PAGE. The strains shared the following characteristics. Cells were ovoid, Gram-positive-staining and non-spore-forming, appearing singly or in pairs or chains. They were facultatively anaerobic and catalase-negative. Growth in broth containing 6.5% NaCl or at 45 6C was weak or absent. Production of D antigen was variable. The strains tolerated 60 6C for 30 min, 40% bile and tellurite, hydrolysed aesculin strongly and gelatin weakly, produced no acid from hippurate and did not reduce it, grew weakly at 10 6C, showed a strong reaction for the Voges–Proskauer test and produced acid from methyl a-D-glucoside, mannitol, sorbitol and sucrose, with weak or no production of acid from methyl a-D-mannoside, L-arabinose, gluconate and L-xylose. Several of the strains were selected for identification on the basis of sequencing of almost the whole 16S rRNA gene and partial atpA and pheS genes and of (GTG)5-PCR fingerprints. Partial atpA and pheS gene sequencing was also performed for those type strains of Enterococcus species without available sequences in the database. The pristine brook isolates formed a novel species, for which the name Enterococcus rivorum sp. nov. (type strain S299T =HAMBI 3055T =LMG 25899T 5CCM 7986T) is proposed. On the basis of 16S rRNA gene sequence similarity, E. rivorum sp. nov. is related to the Enterococcus faecalis genogoup. It is distinguished from described Enterococcus species on the basis of 16S rRNA, atpA and pheS gene sequences and whole-cell protein and (GTG)5-PCR fingerprints. It is most closely related to E. faecalis, but DNA–DNA hybridization confirms it to represent a novel species. ER -
NIEMI, Maarit R., Tuula OLLINKANGAS, Lars PAULIN, Pavel ŠVEC, Peter VANDAMME, Antti KARKMAN, Marcel KOSINA a Kristina LINDSTROM. Enterococcus rivorum sp. nov., from water of pristine brooks. \textit{International Journal of Systematic and Evolutionary Microbiology}. UK: Society for General Microbiology, 2012, roč.~62, č.~9, s.~2169-2173. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijs.0.038257-0.
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