Enterococcus rivorum sp. nov., from water of pristine brooks

J 2012

Enterococcus rivorum sp. nov., from water of pristine brooks

NIEMI, Maarit R., Tuula OLLINKANGAS, Lars PAULIN, Pavel ŠVEC, Peter VANDAMME et. al.

Základní údaje

Originální název

Enterococcus rivorum sp. nov., from water of pristine brooks

Autoři

NIEMI, Maarit R. (246 Finsko), Tuula OLLINKANGAS (246 Finsko), Lars PAULIN (246 Finsko), Pavel ŠVEC (203 Česká republika, garant, domácí), Peter VANDAMME (56 Belgie), Antti KARKMAN (246 Finsko), Marcel KOSINA (203 Česká republika, domácí) a Kristina LINDSTROM (246 Finsko)

Vydání

International Journal of Systematic and Evolutionary Microbiology, UK, Society for General Microbiology, 2012, 1466-5026

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.112

Kód RIV

RIV/00216224:14310/12:00061421

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1099/ijs.0.038257-0

UT WoS

000310048600020

Klíčová slova anglicky

Enterococcus rivorum sp. nov.; pristine brooks; taxonomy

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 4. 2013 21:31, Ing. Andrea Mikešková

Anotace

V originále

A significant number of Enterococcus strains from pristine waters of two brooks in Finland formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional SDS-PAGE. The strains shared the following characteristics. Cells were ovoid, Gram-positive-staining and non-spore-forming, appearing singly or in pairs or chains. They were facultatively anaerobic and catalase-negative. Growth in broth containing 6.5% NaCl or at 45 6C was weak or absent. Production of D antigen was variable. The strains tolerated 60 6C for 30 min, 40% bile and tellurite, hydrolysed aesculin strongly and gelatin weakly, produced no acid from hippurate and did not reduce it, grew weakly at 10 6C, showed a strong reaction for the Voges–Proskauer test and produced acid from methyl a-D-glucoside, mannitol, sorbitol and sucrose, with weak or no production of acid from methyl a-D-mannoside, L-arabinose, gluconate and L-xylose. Several of the strains were selected for identification on the basis of sequencing of almost the whole 16S rRNA gene and partial atpA and pheS genes and of (GTG)5-PCR fingerprints. Partial atpA and pheS gene sequencing was also performed for those type strains of Enterococcus species without available sequences in the database. The pristine brook isolates formed a novel species, for which the name Enterococcus rivorum sp. nov. (type strain S299T =HAMBI 3055T =LMG 25899T 5CCM 7986T) is proposed. On the basis of 16S rRNA gene sequence similarity, E. rivorum sp. nov. is related to the Enterococcus faecalis genogoup. It is distinguished from described Enterococcus species on the basis of 16S rRNA, atpA and pheS gene sequences and whole-cell protein and (GTG)5-PCR fingerprints. It is most closely related to E. faecalis, but DNA–DNA hybridization confirms it to represent a novel species.

Návaznosti

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

NIEMI, Maarit R., Tuula OLLINKANGAS, Lars PAULIN, Pavel ŠVEC, Peter VANDAMME, Antti KARKMAN, Marcel KOSINA a Kristina LINDSTROM. Enterococcus rivorum sp. nov., from water of pristine brooks. International Journal of Systematic and Evolutionary Microbiology. UK: Society for General Microbiology, 2012, roč. 62, č. 9, s. 2169-2173. ISSN 1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijs.0.038257-0.

@article{1070079,
   author = {Niemi, Maarit R. and Ollinkangas, Tuula and Paulin, Lars and Švec, Pavel and Vandamme, Peter and Karkman, Antti and Kosina, Marcel and Lindstrom, Kristina},
   article_location = {UK},
   article_number = {9},
   doi = {http://dx.doi.org/10.1099/ijs.0.038257-0},
   keywords = {Enterococcus rivorum sp. nov.; pristine brooks; taxonomy},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {Enterococcus rivorum sp. nov., from water of pristine brooks},
   volume = {62},
   year = {2012}
}

TY  - JOUR
ID  - 1070079
AU  - Niemi, Maarit R. - Ollinkangas, Tuula - Paulin, Lars - Švec, Pavel - Vandamme, Peter - Karkman, Antti - Kosina, Marcel - Lindstrom, Kristina
PY  - 2012
TI  - Enterococcus rivorum sp. nov., from water of pristine brooks
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 62
IS  - 9
SP  - 2169-2173
EP  - 2169-2173
PB  - Society for General Microbiology
SN  - 14665026
KW  - Enterococcus rivorum sp. nov.
KW  - pristine brooks
KW  - taxonomy
N2  - A significant number of Enterococcus strains from pristine waters of two brooks in Finland formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional SDS-PAGE. The strains shared the following characteristics. Cells were ovoid, Gram-positive-staining and non-spore-forming, appearing singly or in pairs or chains. They were facultatively anaerobic and catalase-negative. Growth in broth containing 6.5% NaCl or at 45 6C was weak or absent. Production of D antigen was variable. The strains tolerated 60 6C for 30 min, 40% bile and tellurite, hydrolysed aesculin strongly and gelatin weakly, produced no acid from hippurate and did not reduce it, grew weakly at 10 6C, showed a strong reaction for the Voges–Proskauer test and produced acid from methyl a-D-glucoside, mannitol, sorbitol and sucrose, with weak or no production of acid from methyl a-D-mannoside, L-arabinose, gluconate and L-xylose. Several of the strains were selected for identification on the basis of sequencing of almost the whole 16S rRNA gene and partial atpA and pheS genes and of (GTG)5-PCR fingerprints. Partial atpA and pheS gene sequencing was also performed for those type strains of Enterococcus species without available sequences in the database. The pristine brook isolates formed a novel species, for which the name Enterococcus rivorum sp. nov. (type strain S299T =HAMBI 3055T =LMG 25899T 5CCM 7986T) is proposed. On the basis of 16S rRNA gene sequence similarity, E. rivorum sp. nov. is related to the Enterococcus faecalis genogoup. It is distinguished from described Enterococcus species on the basis of 16S rRNA, atpA and pheS gene sequences and whole-cell protein and (GTG)5-PCR fingerprints. It is most closely related to E. faecalis, but DNA–DNA hybridization confirms it to represent a novel species.
ER  -

NIEMI, Maarit R., Tuula OLLINKANGAS, Lars PAULIN, Pavel ŠVEC, Peter VANDAMME, Antti KARKMAN, Marcel KOSINA a Kristina LINDSTROM. Enterococcus rivorum sp. nov., from water of pristine brooks. \textit{International Journal of Systematic and Evolutionary Microbiology}. UK: Society for General Microbiology, 2012, roč.~62, č.~9, s.~2169-2173. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijs.0.038257-0.

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