HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC
DETECTION

a 2012

HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION

CERNEI, Natalia Vladimirovna; Markéta KOMINÍKOVÁ; Ondřej ZÍTKA; Jaromír GUMULEC; Michal MASAŘÍK et. al.

Základní údaje

Originální název

HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION

Autoři

Vydání

12th Workshop of Physical Chemists and Electrochemists, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.729 v roce 2011

ISBN

978-80-7375-618-5

ISSN

Klíčová slova anglicky

Sarcosine; Hydrophilic interaction chromatography (HILIC); prostate cancer

Změněno: 29. 10. 2012 16:46, Mgr. Michal Petr

Anotace

V originále

Recently, sarcosine has been identified as a potential prostate cancer marker. To provide early determination of this compound, number of methods is still developed. Sarcosine is relatively small molecule, which is polar enough to enable separation by chromatography with reverse phase. For this reason we optimized method for separation by Hydrophilic interaction chromatography (HILIC). The aim of this work was to suggest the method for HILIC separation and determination of sarcosine with electrochemical detection. This aim was reached as shown by the limit of detection (350 nM), which was determined from calibration curve. This method is suitable for determination of sarcosine in real samples such as urine.

Citovat

CERNEI, Natalia Vladimirovna; Markéta KOMINÍKOVÁ; Ondřej ZÍTKA; Jaromír GUMULEC; Michal MASAŘÍK; Vojtěch ADAM; Jaromír HUBÁLEK; Libuše TRNKOVÁ; Marie STIBOROVÁ; Tomáš ECKSCHLAGER a René KIZEK. HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION. In 12th Workshop of Physical Chemists and Electrochemists. 2012. ISBN 978-80-7375-618-5.

@proceedings{1070200,
   author = {Cernei, Natalia Vladimirovna and Kominíková, Markéta and Zítka, Ondřej and Gumulec, Jaromír and Masařík, Michal and Adam, Vojtěch and Hubálek, Jaromír and Trnková, Libuše and Stiborová, Marie and Eckschlager, Tomáš and Kizek, René},
   booktitle = {12th Workshop of Physical Chemists and Electrochemists},
   keywords = {Sarcosine; Hydrophilic interaction chromatography (HILIC); prostate cancer},
   language = {eng},
   isbn = {978-80-7375-618-5},
   title = {HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION},
   year = {2012}
}

TY  - CONF
ID  - 1070200
AU  - Cernei, Natalia Vladimirovna - Kominíková, Markéta - Zítka, Ondřej - Gumulec, Jaromír - Masařík, Michal - Adam, Vojtěch - Hubálek, Jaromír - Trnková, Libuše - Stiborová, Marie - Eckschlager, Tomáš - Kizek, René
PY  - 2012
TI  - HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION
SN  - 9788073756185
KW  - Sarcosine
KW  - Hydrophilic interaction chromatography (HILIC)
KW  - prostate cancer
N2  - Recently, sarcosine has been identified as a potential prostate cancer marker. To provide early determination of this compound, number of methods is still developed. Sarcosine is relatively small molecule, which is polar enough to enable separation by chromatography with reverse phase. For this reason we optimized method for separation by Hydrophilic interaction chromatography (HILIC). The aim of this work was to suggest the method for HILIC separation and determination of sarcosine with electrochemical detection. This aim was reached as shown by the limit of detection (350 nM), which was determined from calibration curve. This method is suitable for determination of sarcosine in real samples such as urine.
ER  -

CERNEI, Natalia Vladimirovna; Markéta KOMINÍKOVÁ; Ondřej ZÍTKA; Jaromír GUMULEC; Michal MASAŘÍK; Vojtěch ADAM; Jaromír HUBÁLEK; Libuše TRNKOVÁ; Marie STIBOROVÁ; Tomáš ECKSCHLAGER a René KIZEK. HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION. In \textit{12th Workshop of Physical Chemists and Electrochemists}. 2012. ISBN~978-80-7375-618-5.

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