MONITORING THE VIABILITY OF CELL LINE MTF-KO DEPENDING ON THE
CONCENTRATION OF Zn2+ USING XCELLIGENCE SYSTEM

a 2012

MONITORING THE VIABILITY OF CELL LINE MTF-KO DEPENDING ON THE CONCENTRATION OF Zn2+ USING XCELLIGENCE SYSTEM

SZTALMACHOVÁ, Markéta; Veronika TANHÄUSEROVÁ; Marián HLAVNA; Jaromír GUMULEC; Martina RAUDENSKÁ et. al.

Basic information

Original name

MONITORING THE VIABILITY OF CELL LINE MTF-KO DEPENDING ON THE CONCENTRATION OF Zn2+ USING XCELLIGENCE SYSTEM

Authors

SZTALMACHOVÁ, Markéta; Veronika TANHÄUSEROVÁ; Marián HLAVNA; Jaromír GUMULEC; Martina RAUDENSKÁ; Vojtěch ADAM; René KIZEK and Michal MASAŘÍK

Edition

12th Workshop of Physical Chemists and Electrochemists, 2012

Other information

Language

English

Type of outcome

Conference abstract

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 3.729 in 2011

ISBN

978-80-7375-618-5

ISSN

Keywords in English

xCELLigence system; MTF-1 knock-out cell line; zinc ion treatments

Changed: 25/3/2013 14:57, Ing. Mgr. Věra Pospíšilíková

Abstract

In the original language

Metallothioneins (MTs) are low-molecular mass cysteine-rich proteins. Due to thiol bounds in its structure is metallothionein capable to bind metals and thus play a role in metal transportation, cell detoxification and oxidative stress protection. Metallothionein is closely related to zinc metabolism, as zinc is essential compound of mammalian living cells. MT transcription is mediated and controlled thru MTF-1 factor which is activated by metal caused oxidative stress. It is obvious that MTF-1 plays role not only in detoxification and oxidative stress protection, but also in ontogenetic processes. The cell line used in our study was obtained by knock-out of metal regulatory transcription factor 1 (MTF-1) in mouse embryonic fibroblast cell culture MEF-1. We focused on viability monitoring of MTF-KO cell culture viability after zinc ion treatments. This cell line has knock-out metal regulatory transcription factor 1 (MTF-1). Compared to wild type form of mouse embryonic fibroblasts, the growth of MTF-KO cell line is inhibited at lower zinc concentrations.

Links

MUNI/A/0839/2011, interní kód MU

Name: Patofyziologické aspekty vybraných genotypů a fenotypů komplexních nemocí (Acronym: Patofyziologie komplexních nemocí)

Investor: Masaryk University, Category A

Cite

SZTALMACHOVÁ, Markéta; Veronika TANHÄUSEROVÁ; Marián HLAVNA; Jaromír GUMULEC; Martina RAUDENSKÁ; Vojtěch ADAM; René KIZEK and Michal MASAŘÍK. MONITORING THE VIABILITY OF CELL LINE MTF-KO DEPENDING ON THE CONCENTRATION OF Zn2+ USING XCELLIGENCE SYSTEM. In 12th Workshop of Physical Chemists and Electrochemists. 2012. ISBN 978-80-7375-618-5.

@proceedings{1070205,
   author = {Sztalmachová, Markéta and Tanhäuserová, Veronika and Hlavna, Marián and Gumulec, Jaromír and Raudenská, Martina and Adam, Vojtěch and Kizek, René and Masařík, Michal},
   booktitle = {12th Workshop of Physical Chemists and Electrochemists},
   keywords = {xCELLigence system; MTF-1 knock-out cell line; zinc ion treatments},
   language = {eng},
   isbn = {978-80-7375-618-5},
   title = {MONITORING THE VIABILITY OF CELL LINE MTF-KO DEPENDING ON THE CONCENTRATION OF Zn2+ USING XCELLIGENCE SYSTEM},
   year = {2012}
}

TY  - CONF
ID  - 1070205
AU  - Sztalmachová, Markéta - Tanhäuserová, Veronika - Hlavna, Marián - Gumulec, Jaromír - Raudenská, Martina - Adam, Vojtěch - Kizek, René - Masařík, Michal
PY  - 2012
TI  - MONITORING THE VIABILITY OF CELL LINE MTF-KO DEPENDING ON THE CONCENTRATION OF Zn2+ USING XCELLIGENCE SYSTEM
SN  - 9788073756185
KW  - xCELLigence system
KW  - MTF-1 knock-out cell line
KW  - zinc ion treatments
N2  - Metallothioneins (MTs) are low-molecular mass cysteine-rich proteins. Due to thiol bounds in its structure is metallothionein capable to bind metals and thus play a role in metal transportation, cell detoxification and oxidative stress protection. Metallothionein is closely related to zinc metabolism, as zinc is essential compound of mammalian living cells. MT transcription is mediated and controlled thru MTF-1 factor which is activated by metal caused oxidative stress. It is obvious that MTF-1 plays role not only in detoxification and oxidative stress protection, but also in ontogenetic processes. The cell line used in our study was obtained by knock-out of metal regulatory transcription factor 1 (MTF-1) in mouse embryonic fibroblast cell culture MEF-1. We focused on viability monitoring of MTF-KO cell culture viability after zinc ion treatments. This cell line has knock-out metal regulatory transcription factor 1 (MTF-1). Compared to wild type form of mouse embryonic fibroblasts, the growth of MTF-KO cell line is inhibited at lower zinc concentrations.
ER  -

SZTALMACHOVÁ, Markéta; Veronika TANHÄUSEROVÁ; Marián HLAVNA; Jaromír GUMULEC; Martina RAUDENSKÁ; Vojtěch ADAM; René KIZEK and Michal MASAŘÍK. MONITORING THE VIABILITY OF CELL LINE MTF-KO DEPENDING ON THE CONCENTRATION OF Zn2+ USING XCELLIGENCE SYSTEM. In \textit{12th Workshop of Physical Chemists and Electrochemists}. 2012. ISBN~978-80-7375-618-5.

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