Identification of clinical isolates from the genus
Staphylococcus by MALDI-TOF MS and conventional phenothypic
methods

a 2012

Identification of clinical isolates from the genus Staphylococcus by MALDI-TOF MS and conventional phenothypic methods

PETRÁŠ, Petr, Roman PANTŮČEK, Ondrej ŠEDO, Ivana MACHOVÁ, Zbyněk ZDRÁHAL et. al.

Základní údaje

Originální název

Identification of clinical isolates from the genus Staphylococcus by MALDI-TOF MS and conventional phenothypic methods

Autoři

PETRÁŠ, Petr, Roman PANTŮČEK, Ondrej ŠEDO, Ivana MACHOVÁ, Zbyněk ZDRÁHAL a Pavel ŠVEC

Vydání

15 th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2012), Lyon, France, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

MALDI-TOF MS; Staphylococcus species; identification; clinical diagnostics

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 11. 2012 12:03, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Staphylococci in general and S.aureus subsp. aureus in particular are very important human pathogens. More than 400 strains isolated from clinical specimens were identified by MALDI-TOF MS (Microflex; Bruker-Daltonics, Germany) and conventional phenotypic methods (API Staph; STAPHYtest 24; conventional tests). Type strains of 53 staphylococccal species and subspecies were also included in the study. MALDI-TOF MS successfully identified 85% of the clinical isolates to the species level (score >1.900), but not to the subspecies level. The isolates were assigned to 26 species. The MALDI-TOF MS and phenotypic results were concordant in more than 99%. Thirty-one clinical isolates were identified by MALDI-TOF MS as belonging to the complex S. intermedius/S. pseudintermedius. All but one strain of this complex were assigned to S. pseudintermedius by rep-PCR fingerprinting using the (GTG)5 primer. The MALDI-TOF mass spectra-based dendrogram constructed from the data on 53 type strains and data retrieved from the Bruker-Daltonics database revealed that the strains of the same species clustered together and certain branches showed a sufficiently good concordance with the Staphylococcus species groups defined by the phylogenetic analysis of 16S rRNA sequences. Our results indicate that MALDI-TOF MS is a very effective tool for the identification of staphylococci from clinical specimens that has the advantage of being simple, swift, and reliable and requiring low material cost.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA310/09/0459, projekt VaV

Název: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

Investor: Grantová agentura ČR, Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

NT12395, projekt VaV

Název: Molekulární průkaz a analýza invazivních kmenů small colony variants (SCV) a rezistentních kmenů S. aureus od pacientů s cystickou fibrózou

Citovat

PETRÁŠ, Petr, Roman PANTŮČEK, Ondrej ŠEDO, Ivana MACHOVÁ, Zbyněk ZDRÁHAL a Pavel ŠVEC. Identification of clinical isolates from the genus Staphylococcus by MALDI-TOF MS and conventional phenothypic methods. In 15 th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2012), Lyon, France. 2012.

@proceedings{1074374,
   author = {Petráš, Petr and Pantůček, Roman and Šedo, Ondrej and Machová, Ivana and Zdráhal, Zbyněk and Švec, Pavel},
   booktitle = {15 th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2012), Lyon, France},
   keywords = {MALDI-TOF MS; Staphylococcus species; identification; clinical diagnostics},
   language = {eng},
   title = {Identification of clinical isolates from the genus Staphylococcus by MALDI-TOF MS and conventional phenothypic methods},
   url = {http://isssi2012.univ-lyon1.fr/en},
   year = {2012}
}

TY  - CONF
ID  - 1074374
AU  - Petráš, Petr - Pantůček, Roman - Šedo, Ondrej - Machová, Ivana - Zdráhal, Zbyněk - Švec, Pavel
PY  - 2012
TI  - Identification of clinical isolates from the genus Staphylococcus by MALDI-TOF MS and conventional phenothypic methods
KW  - MALDI-TOF MS
KW  - Staphylococcus species
KW  - identification
KW  - clinical diagnostics
UR  - http://isssi2012.univ-lyon1.fr/en
N2  - Staphylococci in general and S.aureus subsp. aureus in particular are very important human pathogens. More than 400 strains isolated from clinical specimens were identified by MALDI-TOF MS (Microflex; Bruker-Daltonics, Germany) and conventional phenotypic methods (API Staph; STAPHYtest 24; conventional tests). Type strains of 53 staphylococccal species and subspecies were also included in the study. MALDI-TOF MS successfully identified 85% of the clinical isolates to the species level (score >1.900), but not to the subspecies level. The isolates were assigned to 26 species. The MALDI-TOF MS and phenotypic results were concordant in more than 99%. Thirty-one clinical isolates were identified by MALDI-TOF MS as belonging to the complex S. intermedius/S. pseudintermedius. All but one strain of this complex were assigned to S. pseudintermedius by rep-PCR fingerprinting using the (GTG)5 primer. The MALDI-TOF mass spectra-based dendrogram constructed from the data on 53 type strains and data retrieved from the Bruker-Daltonics database revealed that the strains of the same species clustered together and certain branches showed a sufficiently good concordance with the Staphylococcus species groups defined by the phylogenetic analysis of 16S rRNA sequences. Our results indicate that MALDI-TOF MS is a very effective tool for the identification of staphylococci from clinical specimens that has the advantage of being simple, swift, and reliable and requiring low material cost.
ER  -

PETRÁŠ, Petr, Roman PANTŮČEK, Ondrej ŠEDO, Ivana MACHOVÁ, Zbyněk ZDRÁHAL a Pavel ŠVEC. Identification of clinical isolates from the genus Staphylococcus by MALDI-TOF MS and conventional phenothypic methods. In \textit{15 th International Symposium on Staphylococci and Staphylococcal Infections (ISSSI2012), Lyon, France}. 2012.

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