MiR-210 expression in tumor tissue and in vitro effects of its
silencing in renal cell carcinoma

J 2013

MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma

RÉDOVÁ, Martina, Alexandr POPRACH, Andrej BEŠŠE, Robert ILIEV, Jana NEKVINDOVÁ et. al.

Základní údaje

Originální název

MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma

Autoři

RÉDOVÁ, Martina (203 Česká republika, domácí), Alexandr POPRACH (203 Česká republika), Andrej BEŠŠE (703 Slovensko, domácí), Robert ILIEV (203 Česká republika, domácí), Jana NEKVINDOVÁ (203 Česká republika), Radek LAKOMÝ (203 Česká republika), Lenka RADOVÁ (203 Česká republika), Marek SVOBODA (203 Česká republika), Jan DOLEŽEL (203 Česká republika), Rostislav VYZULA (203 Česká republika) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

Tumor Biology, Dordrecht, Springer, 2013, 1010-4283

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.840

Kód RIV

RIV/00216224:14740/13:00065569

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1007/s13277-012-0573-2

UT WoS

000313875400056

Klíčová slova anglicky

Renal cell carcinoma; MicroRNA; MiR-210; ACHN; CAKI-2; Proliferation; Invasiveness

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 4. 2014 00:33, Olga Křížová

Anotace

V originále

Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney accounting for about 3 % of adult malignancies. MicroRNAs (miRNAs) are a class of naturally occurring, short non-coding RNAs that regulate gene expression at the post-transcriptional level. We determined global miRNA expression profiles of RCC and parallel renal parenchyma tissues by using quantitative reverse transcriptase-polymerase chain reaction-based TaqMan low-density arrays. Afterward, we validated the difference in miR-210 expression levels on the larger group of RCC patients (35 RCC versus 10 non-tumorous parenchyma samples). Functional in vitro experiments were performed on ACHN and CAKI-2 RCC cell lines transfected with miRNA-210 inhibitor. Cell viability, apoptosis, cell cycle, scratch wound migration assay, and invasion assay (xCELLigence) were performed. We have identified original ccRCC-specific miRNA signature in clinical samples (73 miRNAs were significantly downregulated and five miRNAs upregulated. Increased expression levels of miR-210 in RCC tumor tissue were independently validated. We observed decreased viability of ACHN and CAKI-2 cells and accumulation of CAKI-2 in G2 phase of cell cycle after silencing of miR-210 expression. Downregulation of miR-210 also reduced the migratory and invasive potential of ACHN metastatic RCC cells. Moreover, we showed downregulation of HIF1a protein in both cell lines after miR-210 silencing indicating participation of miR-210 in hypoxic processes of RCC not only through regulation of its target mRNAs but also by indirect regulation of HIF1a. To our knowledge, this is the first report to show miR-210 regulatory effects on cell migration, invasive potential, and HIF1a protein in RCC cells.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

NT13547, projekt VaV

Název: Studium mikroRNA a genů asociovaných s procesem epiteliálně-mezenchymální tranzice jako potenciálních markerů pro predikci rizika a časný záchyt metastazování u pacientů s renálním karcinomem

Citovat

RÉDOVÁ, Martina, Alexandr POPRACH, Andrej BEŠŠE, Robert ILIEV, Jana NEKVINDOVÁ, Radek LAKOMÝ, Lenka RADOVÁ, Marek SVOBODA, Jan DOLEŽEL, Rostislav VYZULA a Ondřej SLABÝ. MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma. Tumor Biology. Dordrecht: Springer, 2013, roč. 34, č. 1, s. 481-491. ISSN 1010-4283. Dostupné z: https://dx.doi.org/10.1007/s13277-012-0573-2.

@article{1075055,
   author = {Rédová, Martina and Poprach, Alexandr and Bešše, Andrej and Iliev, Robert and Nekvindová, Jana and Lakomý, Radek and Radová, Lenka and Svoboda, Marek and Doležel, Jan and Vyzula, Rostislav and Slabý, Ondřej},
   article_location = {Dordrecht},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s13277-012-0573-2},
   keywords = {Renal cell carcinoma; MicroRNA; MiR-210; ACHN; CAKI-2; Proliferation; Invasiveness},
   language = {eng},
   issn = {1010-4283},
   journal = {Tumor Biology},
   title = {MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma},
   volume = {34},
   year = {2013}
}

TY  - JOUR
ID  - 1075055
AU  - Rédová, Martina - Poprach, Alexandr - Bešše, Andrej - Iliev, Robert - Nekvindová, Jana - Lakomý, Radek - Radová, Lenka - Svoboda, Marek - Doležel, Jan - Vyzula, Rostislav - Slabý, Ondřej
PY  - 2013
TI  - MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma
JF  - Tumor Biology
VL  - 34
IS  - 1
SP  - 481-491
EP  - 481-491
PB  - Springer
SN  - 10104283
KW  - Renal cell carcinoma
KW  - MicroRNA
KW  - MiR-210
KW  - ACHN
KW  - CAKI-2
KW  - Proliferation
KW  - Invasiveness
N2  - Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney accounting for about 3 % of adult malignancies. MicroRNAs (miRNAs) are a class of naturally occurring, short non-coding RNAs that regulate gene expression at the post-transcriptional level. We determined global miRNA expression profiles of RCC and parallel renal parenchyma tissues by using quantitative reverse transcriptase-polymerase chain reaction-based TaqMan low-density arrays. Afterward, we validated the difference in miR-210 expression levels on the larger group of RCC patients (35 RCC versus 10 non-tumorous parenchyma samples). Functional in vitro experiments were performed on ACHN and CAKI-2 RCC cell lines transfected with miRNA-210 inhibitor. Cell viability, apoptosis, cell cycle, scratch wound migration assay, and invasion assay (xCELLigence) were performed. We have identified original ccRCC-specific miRNA signature in clinical samples (73 miRNAs were significantly downregulated and five miRNAs upregulated. Increased expression levels of miR-210 in RCC tumor tissue were independently validated. We observed decreased viability of ACHN and CAKI-2 cells and accumulation of CAKI-2 in G2 phase of cell cycle after silencing of miR-210 expression. Downregulation of miR-210 also reduced the migratory and invasive potential of ACHN metastatic RCC cells. Moreover, we showed downregulation of HIF1a protein in both cell lines after miR-210 silencing indicating participation of miR-210 in hypoxic processes of RCC not only through regulation of its target mRNAs but also by indirect regulation of HIF1a. To our knowledge, this is the first report to show miR-210 regulatory effects on cell migration, invasive potential, and HIF1a protein in RCC cells.
ER  -

RÉDOVÁ, Martina, Alexandr POPRACH, Andrej BEŠŠE, Robert ILIEV, Jana NEKVINDOVÁ, Radek LAKOMÝ, Lenka RADOVÁ, Marek SVOBODA, Jan DOLEŽEL, Rostislav VYZULA a Ondřej SLABÝ. MiR-210 expression in tumor tissue and in vitro effects of its silencing in renal cell carcinoma. \textit{Tumor Biology}. Dordrecht: Springer, 2013, roč.~34, č.~1, s.~481-491. ISSN~1010-4283. Dostupné z: https://dx.doi.org/10.1007/s13277-012-0573-2.

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