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@article{1076529, author = {Foltánková, Veronika and Matula, Pavel and Sorokin, Dmitry and Kozubek, Stanislav and Bártová, Eva}, article_location = {Saarbrücken}, article_number = {Issue 2}, doi = {http://dx.doi.org/10.1017/S1431927612014353}, keywords = {DNA damage;chromatin; 53BP1; PML bodies; hybrid detectors; confocal microscopy}, language = {eng}, issn = {1431-9276}, journal = {Microscopy and Microanalysis}, note = {Accepted.}, title = {Hybrid detectors improved time-lapse confocal microscopy of PML and 53BP1 nuclear body co-localization in DNA lesions}, url = {http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8864223}, volume = {Volume 19}, year = {2013} }
TY - JOUR ID - 1076529 AU - Foltánková, Veronika - Matula, Pavel - Sorokin, Dmitry - Kozubek, Stanislav - Bártová, Eva PY - 2013 TI - Hybrid detectors improved time-lapse confocal microscopy of PML and 53BP1 nuclear body co-localization in DNA lesions JF - Microscopy and Microanalysis VL - Volume 19 IS - Issue 2 SP - 360-369 EP - 360-369 PB - Cambridge University Press SN - 14319276 N1 - Accepted. KW - DNA damage;chromatin KW - 53BP1 KW - PML bodies KW - hybrid detectors KW - confocal microscopy UR - http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8864223 L2 - http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8864223 N2 - We used hybrid detectors (HyDs) to monitor the trajectories and interactions of promyelocytic leukemia (GFP-PML) nuclear bodies (NBs) and mCherry-53BP1-positive DNA lesions. 53BP1 protein accumulates in NBs that occur spontaneously in the genome or in gamma-irradiationinduced foci. When we induced local DNA damage by ultraviolet irradiation, we also observed accumulation of 53BP1 proteins into discrete bodies, instead of the expected dispersed pattern. In comparison with photomultiplier tubes (PMTs), which are used for standard analysis by confocal laser scanning microscopy, HyDs significantly eliminated photobleaching of GFP and mCherry fluorochromes during image acquisition. The low laser intensities used for HyD-based confocal analysis enabled us to observe NBs for the longer time periods, necessary for studies of the trajectories and interactions of PML and 53BP1 NBs. To further characterize protein interactions, we used resonance scanning and a novel bioinformatics approach to register and analyze the movements of individual PML and 53BP1 NBs. The combination of improved HyD-based confocal microscopy with a tailored bioinformatics approach enabled us to reveal damage-specific properties of PML and 53BP1 NBs. ER -
FOLTÁNKOVÁ, Veronika, Pavel MATULA, Dmitry SOROKIN, Stanislav KOZUBEK a Eva BÁRTOVÁ. Hybrid detectors improved time-lapse confocal microscopy of PML and 53BP1 nuclear body co-localization in DNA lesions. \textit{Microscopy and Microanalysis}. Saarbrücken: Cambridge University Press, 2013, Volume 19, Issue 2, s.~360-369. ISSN~1431-9276. Dostupné z: https://dx.doi.org/10.1017/S1431927612014353.
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