D 2012

Development of capillary electrophoretic method for nucleotide and coenzyme analysis in bacterial and stem cells

MUSILOVÁ, Jindra and Zdeněk GLATZ

Basic information

Original name

Development of capillary electrophoretic method for nucleotide and coenzyme analysis in bacterial and stem cells

Name in Czech

Vývoj kapilárně elektroforetické metody pro stanovení nukleotidů a koenzymů v bakteriálních a kmenových buňkách

Authors

Edition

CHEMICA 50S, p. 97-98, 2 pp. 2012

Publisher

Faculty of Science, Palacký University Olomouc

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

ISBN

978-80-244-3115-4

ISSN

Keywords (in Czech)

kapilární elektroforéza, nukleotidy, koenzymy

Keywords in English

capillary electrophoresis, nucleotides, coenzymes
Změněno: 21/1/2014 15:04, prof. RNDr. Zdeněk Glatz, CSc.

Abstract

ORIG CZ

V originále

Metabolite profiling is one of the major approaches for metabolomics studies. It involves the identification and quantitation of a group of metabolites common to a specific pathway or chemical class. Nucleotides and coenzymes represent central energetic metabolites in the complex metabolic network. As a consequence, the quantitative determination of such important compounds is fundamental in many areas of biochemical research. The concentrations of the nucleotides and coenzymes in living cells are in the micromolar concentration range therefore highly sensitive separation techniques are required for their determination. A combination of capillary zone electrophoresis (CZE) with probably the most extensively used on-line preconcentration technique – field enhanced sample stacking – allows their analysis in different cell types. Appropriate conditions for selective separation of 17 energetically important metabolites (purine, pyrimidine nucleotides, adenine coenzymes and Acetyl CoA) were found. The most dominant parameter for the analysis was pH of the background electrolyte. The optimized methodology was applied on the cell-extract of bacterium Paracoccus denitrificans and stem cell extract. The described method is suitable for determination of large group of energetically important metabolites and its utilisation depends on character of real cell samples.

In Czech

Byly nalezeny vhodné podmínky pro separaci purinových a pyrimidinových nukleotidů, adeninových koenzymů a acetylCoA na kapilární elektroforéze. Pro zvýšení koncentrační citlivosti byla CE kombinována s on-line prekoncentrací - field enhanced stacking.Optimalizovaná metoda byla aplikována na bezbuněčné extrakty bakterie P. denitrificans a extrakty kmenových buněk.

Links

CZ.1.07/2.3.00/20.0182, interní kód MU
(CEP code: EE2.3.20.0182)
Name: BBC - Vytvoření multidisciplinárního výzkumného a vzdělávacího centra bioanalytických technologií (Acronym: BBC)
Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development
GAP206/11/0009, research and development project
Name: Kapilární electroforéza jako nástroj pro metabolomické studie (Acronym: CE metabolomics)
Investor: Czech Science Foundation