Determination of oxidative stress and activities of antioxidant
enzymes in guinea pigs treated with haloperidol

J 2013

Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol

GUMULEC, Jaromír, Martina RAUDENSKÁ, Marián HLAVNA, Tibor STRAČINA, Markéta SZTALMACHOVÁ et. al.

Základní údaje

Originální název

Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol

Autoři

GUMULEC, Jaromír (203 Česká republika, domácí), Martina RAUDENSKÁ (203 Česká republika, domácí), Marián HLAVNA (703 Slovensko, domácí), Tibor STRAČINA (703 Slovensko, domácí), Markéta SZTALMACHOVÁ (203 Česká republika, domácí), Veronika TANHÄUSEROVÁ (203 Česká republika, domácí), Lukáš PÁCAL (203 Česká republika, domácí), Branislav RUTTKAY-NEDECKÝ (203 Česká republika), Jiří SOCHOR (203 Česká republika), Ondřej ZÍTKA (203 Česká republika), Petr BABULA (203 Česká republika), Vojtěch ADAM (203 Česká republika), René KIZEK (203 Česká republika), Marie NOVÁKOVÁ (203 Česká republika, domácí) a Michal MASAŘÍK (203 Česká republika, garant, domácí)

Vydání

Experimental and Therapeutic Medicine, Athens, Spandidos Publications Ltd. 2013, 1792-0981

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.941

Kód RIV

RIV/00216224:14110/13:00067578

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3892/etm.2012.822

UT WoS

000313764200021

Klíčová slova anglicky

haloperidol; guinea pig; oxidative stress; superoxide dismutase; glutathione reductase; glutathione-S-transferase

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 2. 2014 14:54, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Guinea pigs (Cavia porcellus) were treated with haloperidol (HP), and free radical (FR) and ferric reducing antioxidant power (FRAP) assays were used to determine oxidative stress levels. Furthermore, the superoxide dismutase (SOD), glutathione reductase (GR) and glutathione-S-transferase (GST) activity levels were detected and glucose levels and the reduced and oxidized glutathione (GSH/GSSG) ratio were measured in HP-treated and untreated guinea pigs. The present study demonstrated that the administration of HP causes significant oxidative stress in guinea pigs (P=0,022). In animals treated with HP, the activity of GST was significantly increased compared with a placebo (P=0,007). The elevation of SOD and GR activity levels and increase in the levels of glutathione (GSH) in HP-treated animals were not statistically significant. In the HP-untreated animals, a significant positive correlation was observed between oxidative stress detected by the FR method and GST (r=0,88, P=0,008) and SOD (r=0,86, P=0,01) activity levels, respectively. A significant negative correlation between the levels of plasma glucose and oxidative stress detected by the FRAP method was observed (r=-0,78, P=0,04). Notably, no significant correlations were observed in the treated animals. In the HP-treated group, two subgroups of animals were identified according to their responses to oxidative stress. The group with higher levels of plasma HP had higher enzyme activity and reactive oxygen species production compared with the group with lower plasma levels of HP. The greatest difference in activity (U/microl) between the two groups of animals was for GR.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

MUNI/A/0846/2011, interní kód MU

Název: Studium mechanismů vzniku některých kardiovaskulárních onemocnění (Akronym: SMEKAON)

Investor: Masarykova univerzita, Studium mechanismů vzniku některých kardiovaskulárních onemocnění, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Přiložené soubory

Gumulec.pdf

Požádat o autorskou verzi souboru

Citovat

GUMULEC, Jaromír, Martina RAUDENSKÁ, Marián HLAVNA, Tibor STRAČINA, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Lukáš PÁCAL, Branislav RUTTKAY-NEDECKÝ, Jiří SOCHOR, Ondřej ZÍTKA, Petr BABULA, Vojtěch ADAM, René KIZEK, Marie NOVÁKOVÁ a Michal MASAŘÍK. Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol. Experimental and Therapeutic Medicine. Athens: Spandidos Publications Ltd., 2013, roč. 5, č. 2, s. 479-484. ISSN 1792-0981. Dostupné z: https://dx.doi.org/10.3892/etm.2012.822.

@article{1079005,
   author = {Gumulec, Jaromír and Raudenská, Martina and Hlavna, Marián and Stračina, Tibor and Sztalmachová, Markéta and Tanhäuserová, Veronika and Pácal, Lukáš and RuttkayandNedecký, Branislav and Sochor, Jiří and Zítka, Ondřej and Babula, Petr and Adam, Vojtěch and Kizek, René and Nováková, Marie and Masařík, Michal},
   article_location = {Athens},
   article_number = {2},
   doi = {http://dx.doi.org/10.3892/etm.2012.822},
   keywords = {haloperidol; guinea pig; oxidative stress; superoxide dismutase; glutathione reductase; glutathione-S-transferase},
   language = {eng},
   issn = {1792-0981},
   journal = {Experimental and Therapeutic Medicine},
   title = {Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol},
   volume = {5},
   year = {2013}
}

TY  - JOUR
ID  - 1079005
AU  - Gumulec, Jaromír - Raudenská, Martina - Hlavna, Marián - Stračina, Tibor - Sztalmachová, Markéta - Tanhäuserová, Veronika - Pácal, Lukáš - Ruttkay-Nedecký, Branislav - Sochor, Jiří - Zítka, Ondřej - Babula, Petr - Adam, Vojtěch - Kizek, René - Nováková, Marie - Masařík, Michal
PY  - 2013
TI  - Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol
JF  - Experimental and Therapeutic Medicine
VL  - 5
IS  - 2
SP  - 479-484
EP  - 479-484
PB  - Spandidos Publications Ltd.
SN  - 17920981
KW  - haloperidol
KW  - guinea pig
KW  - oxidative stress
KW  - superoxide dismutase
KW  - glutathione reductase
KW  - glutathione-S-transferase
N2  - Guinea pigs (Cavia porcellus) were treated with haloperidol (HP), and free radical (FR) and ferric reducing antioxidant power (FRAP) assays were used to determine oxidative stress levels. Furthermore, the superoxide dismutase (SOD), glutathione reductase (GR) and glutathione-S-transferase (GST) activity levels were detected and glucose levels and the reduced and oxidized glutathione (GSH/GSSG) ratio were measured in HP-treated and untreated guinea pigs. The present study demonstrated that the administration of HP causes significant oxidative stress in guinea pigs (P=0,022). In animals treated with HP, the activity of GST was significantly increased compared with a placebo (P=0,007). The elevation of SOD and GR activity levels and increase in the levels of glutathione (GSH) in HP-treated animals were not statistically significant. In the HP-untreated animals, a significant positive correlation was observed between oxidative stress detected by the FR method and GST (r=0,88, P=0,008) and SOD (r=0,86, P=0,01) activity levels, respectively. A significant negative correlation between the levels of plasma glucose and oxidative stress detected by the FRAP method was observed (r=-0,78, P=0,04). Notably, no significant correlations were observed in the treated animals. In the HP-treated group, two subgroups of animals were identified according to their responses to oxidative stress. The group with higher levels of plasma HP had higher enzyme activity and reactive oxygen species production compared with the group with lower plasma levels of HP. The greatest difference in activity (U/microl) between the two groups of animals was for GR.
ER  -

GUMULEC, Jaromír, Martina RAUDENSKÁ, Marián HLAVNA, Tibor STRAČINA, Markéta SZTALMACHOVÁ, Veronika TANHÄUSEROVÁ, Lukáš PÁCAL, Branislav RUTTKAY-NEDECKÝ, Jiří SOCHOR, Ondřej ZÍTKA, Petr BABULA, Vojtěch ADAM, René KIZEK, Marie NOVÁKOVÁ a Michal MASAŘÍK. Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol. \textit{Experimental and Therapeutic Medicine}. Athens: Spandidos Publications Ltd., 2013, roč.~5, č.~2, s.~479-484. ISSN~1792-0981. Dostupné z: https://dx.doi.org/10.3892/etm.2012.822.

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