FARKA, Zdeněk, David KOVÁŘ, Jan PŘIBYL and Petr SKLÁDAL. Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores. International Journal of Electrochemical Science. Belgrade, Serbia: ESG, 2013, vol. 8, No 1, p. 100-112. ISSN 1452-3981.
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Basic information
Original name Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores
Name in Czech Piezoelektrické a SPR biosensory pro stanoveni spor Bacillus atrophaeus
Authors FARKA, Zdeněk (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
Edition International Journal of Electrochemical Science, Belgrade, Serbia, ESG, 2013, 1452-3981.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Serbia
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.956
RIV identification code RIV/00216224:14310/13:00067588
Organization unit Faculty of Science
UT WoS 000316562800008
Keywords (in Czech) biosensor; spory; piezosensor
Keywords in English Bacillus atrophaeus spore; Biosensor; Immunosensor; Quartz Crystal Microbalance; Surface Plasmon Resonance
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 25/3/2014 11:16.
Abstract
Cheap, rapid and user friendly tools for detection of microorganisms were intensively discussed during last decades. The use of immunospecific biosensors seems advantageous for samples originating from clinical, environmental and agricultural area, food processing and military. The piezoelectric (quartz crystal microbalance, QCM) and optical (surface plasmon resonance, SPR) biosensors represented by QCM Analyzer and Biacore 3000 were compared for future development of real-time and label-free microbial biosensors. The spores of Bacillus atrophaeus were used as a non-pathogenic surrogate for B. anthracis. Seven commercially available antibodies were immobilised on the sensor surfaces to select the most promising one. Various immobilisation techniques were used. The total time of analysis did not exceed 30 min and the estimated limits of detection (LOD) were 10^6 CFU mL-1 and 10^5 CFU mL-1 in case of QCM and SPR, respectively.
Abstract (in Czech)
Imunospecifické biosensory - imunosensory - pro detekci spor Bacillus atrophaeus, konstrukce na bázi piezosensorů a plasmonové resonance.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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