2011
Development of Cryptosporidium muris in experimental conditions: preliminary study.
MELICHEROVÁ, Janka, Jana ILGOVÁ, Martin KVÁČ a Andrea VALIGUROVÁZákladní údaje
Originální název
Development of Cryptosporidium muris in experimental conditions: preliminary study.
Název česky
Development of Cryptosporidium muris in experimental conditions: preliminary study.
Název anglicky
Development of Cryptosporidium muris in experimental conditions: preliminary study.
Autoři
MELICHEROVÁ, Janka (703 Slovensko, garant, domácí), Jana ILGOVÁ (703 Slovensko, domácí), Martin KVÁČ (203 Česká republika) a Andrea VALIGUROVÁ (703 Slovensko)
Vydání
41. Jírovec Protozoological Days, Benecko v Krkonoších, May 9-13, 2011. 2011
Další údaje
Jazyk
čeština
Typ výsledku
Konferenční abstrakt
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/11:00058215
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
cryptosporidia; life cycle; rodent
Klíčová slova anglicky
cryptosporidia; life cycle; rodent
Změněno: 12. 2. 2014 15:02, Mgr. et Mgr. Jana Ilgová, Ph.D.
V originále
Cryptosporidia (Apicomplexa) have been recently recognized as one of the most ubiquitous parasites infecting gastrointestinal and respiratory tract of vertebrates. Localization of cryptosporidian developmental stages in the host epithelium has been the subject of extensive speculation for many years. Our published data support the term epicellular to reflect the cryptosporidian localization, rather than the term intracellular extracytoplasmic, used throughout the literature. Due to its peculiar localization, Cryptosporidium can derive nutrition while minimizing immunologic recognition and there is currently no effective treatment for cryptosporidiosis. In presented study, the life cycle of Cryptosporidium muris parasitizing gastric glands of experimentally infected laboratory rodents, Mastomys natalensis and BALB/c mice, has been analysed in detail. The main aim was to map the development of C. muris in hosts exhibiting different response to the parasitization. We have focused on the endogenous development of C. muris, during which the parasite was attached to the host epithelium and its development included both asexual and sexual developmental stages. Animals were dissected and screened for the presence of parasite using combined morphological approach and nested PCR (SSU rRNA) at different time after inoculation with infective oocysts. The study has revealed possible difficulties in detecting C. muris developmental stages in gastric glands during the first days of parasitization.
Anglicky
Cryptosporidia (Apicomplexa) have been recently recognized as one of the most ubiquitous parasites infecting gastrointestinal and respiratory tract of vertebrates. Localization of cryptosporidian developmental stages in the host epithelium has been the subject of extensive speculation for many years. Our published data support the term epicellular to reflect the cryptosporidian localization, rather than the term intracellular extracytoplasmic, used throughout the literature. Due to its peculiar localization, Cryptosporidium can derive nutrition while minimizing immunologic recognition and there is currently no effective treatment for cryptosporidiosis. In presented study, the life cycle of Cryptosporidium muris parasitizing gastric glands of experimentally infected laboratory rodents, Mastomys natalensis and BALB/c mice, has been analysed in detail. The main aim was to map the development of C. muris in hosts exhibiting different response to the parasitization. We have focused on the endogenous development of C. muris, during which the parasite was attached to the host epithelium and its development included both asexual and sexual developmental stages. Animals were dissected and screened for the presence of parasite using combined morphological approach and nested PCR (SSU rRNA) at different time after inoculation with infective oocysts. The study has revealed possible difficulties in detecting C. muris developmental stages in gastric glands during the first days of parasitization.
Návaznosti
| GPP506/10/P372, projekt VaV |
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