J 2012

"Click & Seed" Approach to the Biomimetic Modification of Material Surfaces

PROKS, Vladimír; Josef JAROŠ; Ognen POP-GEORGIEVSKI; Jan KUČKA; Štěpán POPELKA et. al.

Basic information

Original name

"Click & Seed" Approach to the Biomimetic Modification of Material Surfaces

Authors

PROKS, Vladimír (203 Czech Republic, guarantor); Josef JAROŠ (203 Czech Republic, belonging to the institution); Ognen POP-GEORGIEVSKI (203 Czech Republic); Jan KUČKA (203 Czech Republic); Štěpán POPELKA (203 Czech Republic); Petr DVOŘÁK (203 Czech Republic, belonging to the institution); Aleš HAMPL (203 Czech Republic, belonging to the institution) and František RYPÁČEK (203 Czech Republic)

Edition

Macromolecular Bioscience, Weinheim, Wiley-VCH Verlag, 2012, 1616-5187

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

Genetics and molecular biology

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 3.742

RIV identification code

RIV/00216224:14110/12:00063122

Organization unit

Faculty of Medicine

DOI

UT WoS

000308393100010

Keywords in English

biomimetic modifications; click chemistry; peptide radiolabeling; polydopamine; poly(ethylene oxide)

Tags

International impact, Reviewed
Changed: 22/4/2013 17:49, Ing. Mgr. Věra Pospíšilíková

Abstract

In the original language

A simple, versatile, protein-repulsive, substrate-independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow-up modifications with various ligands using a coppercatalyzed alkyne-azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of 125I-radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line.

Links

MSM0021622430, plan (intention)
Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie
Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
MUNI/A/0933/2010, interní kód MU
Name: Zdroje pro tkáňové inženýrství
Investor: Masaryk University, Category A