Upregulation of IL-6 and its signaling molecules after nerve
injury: the concept of Yin Yang for reinnervation and induction
of neuropathic pain

p 2012

Upregulation of IL-6 and its signaling molecules after nerve injury: the concept of Yin Yang for reinnervation and induction of neuropathic pain

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Lucie STREJČKOVÁ et. al.

Základní údaje

Originální název

Upregulation of IL-6 and its signaling molecules after nerve injury: the concept of Yin Yang for reinnervation and induction of neuropathic pain

Název česky

Zvýšená produkce IL-6 a jeho signálních molekul po poškození periferního nervu: pojem jin a jang v souvislosti s reinervací a indukci neuropatické bolesti

Autoři

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA a Lucie STREJČKOVÁ

Vydání

Morphology 2012, 2012

Další údaje

Typ výsledku

Vyžádané přednášky

Obor

30000 3. Medical and Health Sciences

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

Jin a jang; chronická komprese nervu; Wallerova degenerace; regenerace poškozených axonů; neuropatická bolest

Klíčová slova anglicky

Yin and yang; chronic constriction; Wallerian degeneration; regeneration of damaged axons; neuropathic pain

Příznaky

Mezinárodní význam

Změněno: 23. 12. 2013 15:21, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

In Asian philosophy, the concept of Yin Yang is used to describe how seemingly contrary forces are interconnected and interdependent in the natural world, and how they give rise to each other in turn. Opposites thus only exist in relation to each other. Wallerian degeneration is a cascade of stereotype cellular events and molecular alterations distal to injury of nerve fibers including upregulation of inflammatory mediators like cytokines. These events may prepare conditions for both regeneration of damaged axons and induction of neuropathic pain. The goal of our present experiments was to study IL-6 and its signaling molecules in the model of peripheral nerve injury in relation to regrowing axons and induction of neuropathic pain. Unilateral chronic constriction injury (CCI) of the sciatic nerve was performed aseptically in sixty four rats. Neuropathic pain induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Expression of IL-6, IL-6R, gp130, STAT-3 and SOCS3 were investigated bilaterally in the sciatic nerve as well as in both lumbar (L4-5) and cervical (C7-8) dorsal root ganglia (DRG) following CCI for 1, 3, 7 and 14 days. Regrowing axons were detected simultaneously by GAP-43 immunostaining. The results of immunofluorescence staining revealed that activated Schwann cells distal to nerve constriction and satellite glial cells of DRG are robust source for IL-6 and molecules for its signal transduction and regulation. Activated Schwann cells that displayed IL-6 protein and its signal molecules were found in close contact with growing axons as well as in contralateral homonymous nerve. Activated satellite glial cells producing inflammatory mediators are involved in ectopic activation of the primary sensory neurons or their neuroprotection. Both beneficial and detrimental effects of IL6 distal to nerve injury and in DRG have to be considered in a strategy of axon promotion on one side and suppression of the initial stages of neuropathic pain on other side. Some recent knowledge on this topic is stressed.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA a Lucie STREJČKOVÁ. Upregulation of IL-6 and its signaling molecules after nerve injury: the concept of Yin Yang for reinnervation and induction of neuropathic pain. In Morphology 2012. 2012.

@misc{1085447,
   author = {Dubový, Petr and Hradilová Svíženská, Ivana and Klusáková, Ilona and Brázda, Václav and Strejčková, Lucie},
   booktitle = {Morphology 2012},
   keywords = {Yin and yang; chronic constriction; Wallerian degeneration; regeneration of damaged axons; neuropathic pain},
   title = {Upregulation of IL-6 and its signaling molecules after nerve injury: the concept of Yin Yang for reinnervation and induction of neuropathic pain},
   url = {http://cas.lf1.cuni.cz/sjezd/program.pdf},
   year = {2012}
}

TY  - SLIDE
ID  - 1085447
AU  - Dubový, Petr - Hradilová Svíženská, Ivana - Klusáková, Ilona - Brázda, Václav - Strejčková, Lucie
PY  - 2012
TI  - Upregulation of IL-6 and its signaling molecules after nerve injury: the concept of Yin Yang for reinnervation and induction of neuropathic pain
KW  - Yin and yang
KW  - chronic constriction
KW  - Wallerian degeneration
KW  - regeneration of damaged axons
KW  - neuropathic pain
UR  - http://cas.lf1.cuni.cz/sjezd/program.pdf
N2  - In Asian philosophy, the concept of Yin Yang is used to describe how seemingly contrary forces are interconnected and interdependent in the natural world, and how they give rise to each other in turn. Opposites thus only exist in relation to each other. Wallerian degeneration is a cascade of stereotype cellular events and molecular alterations distal to injury of nerve fibers including upregulation of inflammatory mediators like cytokines. These events may prepare conditions for both regeneration of damaged axons and induction of neuropathic pain. The goal of our present experiments was to study IL-6 and its signaling molecules in the model of peripheral nerve injury in relation to regrowing axons and induction of neuropathic pain. Unilateral chronic constriction injury (CCI) of the sciatic nerve was performed aseptically in sixty four rats. Neuropathic pain induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Expression of IL-6, IL-6R, gp130, STAT-3 and SOCS3 were investigated bilaterally in the sciatic nerve as well as in both lumbar (L4-5) and cervical (C7-8) dorsal root ganglia (DRG) following CCI for 1, 3, 7 and 14 days. Regrowing axons were detected simultaneously by GAP-43 immunostaining. The results of immunofluorescence staining revealed that activated Schwann cells distal to nerve constriction and satellite glial cells of DRG are robust source for IL-6 and molecules for its signal transduction and regulation. Activated Schwann cells that displayed IL-6 protein and its signal molecules were found in close contact with growing axons as well as in contralateral homonymous nerve. Activated satellite glial cells producing inflammatory mediators are involved in ectopic activation of the primary sensory neurons or their neuroprotection. Both beneficial and detrimental effects of IL6 distal to nerve injury and in DRG have to be considered in a strategy of axon promotion on one side and suppression of the initial stages of neuropathic pain on other side. Some recent knowledge on this topic is stressed.
ER  -

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