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@article{1086093, author = {Kubesová, Anna and Šalplachta, Jiří and Horká, Marie and Růžička, Filip and Šlais, Karel}, article_location = {Cambridge}, article_number = {8}, doi = {http://dx.doi.org/10.1039/c2an15931g}, keywords = {DESORPTION IONIZATION-TIME; RAPID IDENTIFICATION; BIOFILM PRODUCTION; PARAPSILOSIS; ORTHOPSILOSIS; METAPSILOSIS; SEPARATION; DIVERSITY; INFECTION; PROTEINS}, language = {eng}, issn = {0003-2654}, journal = {Analyst}, title = {Candida "Psilosis" - electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination}, url = {http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an15931g}, volume = {137}, year = {2012} }
TY - JOUR ID - 1086093 AU - Kubesová, Anna - Šalplachta, Jiří - Horká, Marie - Růžička, Filip - Šlais, Karel PY - 2012 TI - Candida "Psilosis" - electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination JF - Analyst VL - 137 IS - 8 SP - 1937-1943 EP - 1937-1943 PB - The Royal Society of Chemistry SN - 00032654 KW - DESORPTION IONIZATION-TIME KW - RAPID IDENTIFICATION KW - BIOFILM PRODUCTION KW - PARAPSILOSIS KW - ORTHOPSILOSIS KW - METAPSILOSIS KW - SEPARATION KW - DIVERSITY KW - INFECTION KW - PROTEINS UR - http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an15931g L2 - http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an15931g N2 - Biofilm-positive strains of Candida parapsilosis are the second most common yeasts responsible for bloodstream infections. This pathogen is difficult to identify by standard methods from other phenotypically indistinguishable species, biofilm-negative Candida orthopsilosis and biofilm-positive Candida metapsilosis. From a medical point of view, important information is especially whether the strains form biofilm. The biofilm formation enables yeast to colonize artificial surfaces thereby protecting the yeast cell against antifungal agents. The commonly used genotypic methods including different modifications of the polymerase chain reaction have some disadvantages. Therefore, a rapid and reliable method able to identify phenotypically indistinguishable C. "psilosis" species is still of great interest. In this study, the four well-established analytical techniques: gel isoelectric focusing, sodium dodecyl sulfate polyacrylamide gel electrophoresis, two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, were applied in order to discriminate C. "psilosis" species. The ability of these techniques to differentiate between biofilm-positive and biofilm-negative strains was further investigated. Our results have revealed that the proposed methods, especially matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of intact yeast cells, can be used as the efficient tools for discrimination and identification of the phenotypically indistinguishable microorganisms. ER -
KUBESOVÁ, Anna, Jiří ŠALPLACHTA, Marie HORKÁ, Filip RŮŽIČKA a Karel ŠLAIS. Candida ''Psilosis'' - electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination. \textit{Analyst}. Cambridge: The Royal Society of Chemistry, 2012, roč.~137, č.~8, s.~1937-1943. ISSN~0003-2654. Dostupné z: https://dx.doi.org/10.1039/c2an15931g.
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