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@article{1086093,
author = {Kubesová, Anna and Šalplachta, Jiří and Horká, Marie and Růžička, Filip and Šlais, Karel},
article_location = {Cambridge},
article_number = {8},
doi = {http://dx.doi.org/10.1039/c2an15931g},
keywords = {DESORPTION IONIZATION-TIME; RAPID IDENTIFICATION; BIOFILM PRODUCTION; PARAPSILOSIS; ORTHOPSILOSIS; METAPSILOSIS; SEPARATION; DIVERSITY; INFECTION; PROTEINS},
language = {eng},
issn = {0003-2654},
journal = {Analyst},
title = {Candida "Psilosis" - electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination},
url = {http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an15931g},
volume = {137},
year = {2012}
}
TY - JOUR
ID - 1086093
AU - Kubesová, Anna - Šalplachta, Jiří - Horká, Marie - Růžička, Filip - Šlais, Karel
PY - 2012
TI - Candida "Psilosis" - electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination
JF - Analyst
VL - 137
IS - 8
SP - 1937-1943
EP - 1937-1943
PB - The Royal Society of Chemistry
SN - 00032654
KW - DESORPTION IONIZATION-TIME
KW - RAPID IDENTIFICATION
KW - BIOFILM PRODUCTION
KW - PARAPSILOSIS
KW - ORTHOPSILOSIS
KW - METAPSILOSIS
KW - SEPARATION
KW - DIVERSITY
KW - INFECTION
KW - PROTEINS
UR - http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an15931g
L2 - http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an15931g
N2 - Biofilm-positive strains of Candida parapsilosis are the second most common yeasts responsible for bloodstream infections. This pathogen is difficult to identify by standard methods from other phenotypically indistinguishable species, biofilm-negative Candida orthopsilosis and biofilm-positive Candida metapsilosis. From a medical point of view, important information is especially whether the strains form biofilm. The biofilm formation enables yeast to colonize artificial surfaces thereby protecting the yeast cell against antifungal agents. The commonly used genotypic methods including different modifications of the polymerase chain reaction have some disadvantages. Therefore, a rapid and reliable method able to identify phenotypically indistinguishable C. "psilosis" species is still of great interest. In this study, the four well-established analytical techniques: gel isoelectric focusing, sodium dodecyl sulfate polyacrylamide gel electrophoresis, two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, were applied in order to discriminate C. "psilosis" species. The ability of these techniques to differentiate between biofilm-positive and biofilm-negative strains was further investigated. Our results have revealed that the proposed methods, especially matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of intact yeast cells, can be used as the efficient tools for discrimination and identification of the phenotypically indistinguishable microorganisms.
ER -
KUBESOVÁ, Anna, Jiří ŠALPLACHTA, Marie HORKÁ, Filip RŮŽIČKA a Karel ŠLAIS. Candida ''Psilosis'' - electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination. \textit{Analyst}. Cambridge: The Royal Society of Chemistry, 2012, roč.~137, č.~8, s.~1937-1943. ISSN~0003-2654. Dostupné z: https://dx.doi.org/10.1039/c2an15931g.
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