Loss of the oligosaccharyl transferase subunit TUSC3 promotes
proliferation and migration of ovarian cancer cells

J 2013

Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells

VAŇHARA, Petr, Peter HORAK, Dietmar PILS, Mariam ANEES, Michaela PETZ et. al.

Základní údaje

Originální název

Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells

Autoři

VAŇHARA, Petr (203 Česká republika, garant, domácí), Peter HORAK (40 Rakousko), Dietmar PILS (40 Rakousko), Mariam ANEES (40 Rakousko), Michaela PETZ (40 Rakousko), Wolfgang GREGOR (40 Rakousko), Robert ZEILLINGER (40 Rakousko) a Michael KRAINER (40 Rakousko)

Vydání

International Journal of Oncology, Athens, SPANDIDOS PUBL LTD, 2013, 1019-6439

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.773

Kód RIV

RIV/00216224:14110/13:00081853

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3892/ijo.2013.1824

UT WoS

000316511200030

Klíčová slova anglicky

TUSC3; ovarian cancer; glycosylation; proliferation

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 6. 2015 12:19, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Consequences of deregulated protein N-glycosylation on cancer pathogenesis are poorly understood. TUSC3 is a gene with a putative function in N-glycosylation, located on the short arm of chromosome 8. This is a chromosomal region of frequent genetic loss in ovarian cancer. We established recently that the expression of TUSC3 is epigenetically decreased in epithelial ovarian cancer compared to benign controls and provides prognostic information on patient survival. Therefore, we analyzed the consequences of silenced TUSC3 expression on proliferation, invasion and migration of ovarian cell lines. In addition, we performed subcellular fractionation, co-immunofluorescence and co-immunoprecipitation experiments to establish the molecular localization of TUSC3 in ovarian cancer cells. We demonstrated that TUSC3 is localized in the endoplasmic reticulum as a subunit of the oligosaccharyltransferase complex and is capable of modulation of glycosylation patterning of ovarian cancer cells. Most importantly, silencing of TUSC3 enhances proliferation and migration of ovarian cancer cells in vitro. Our observations suggest a role for N-glycosylating events in ovarian cancer pathogenesis in general, and identify TUSC3 as a tumor suppressor gene in ovarian cancer in particular.

Návaznosti

EE2.3.20.0185, projekt VaV

Název: Centrum analýz a modelování tkání a orgánů

7AMB12AT019, projekt VaV

Název: Studium molekulární podstaty nových tumorových markerů u ovariálních a prostatických karcinomů (Akronym: Biomarkers)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Studium molekulární podstaty nových tumorových markerů u ovariálních a prostatických karcinomů

Citovat

VAŇHARA, Petr, Peter HORAK, Dietmar PILS, Mariam ANEES, Michaela PETZ, Wolfgang GREGOR, Robert ZEILLINGER a Michael KRAINER. Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells. International Journal of Oncology. Athens: SPANDIDOS PUBL LTD, 2013, roč. 42, č. 4, s. 1383-1389. ISSN 1019-6439. Dostupné z: https://dx.doi.org/10.3892/ijo.2013.1824.

@article{1086737,
   author = {Vaňhara, Petr and Horak, Peter and Pils, Dietmar and Anees, Mariam and Petz, Michaela and Gregor, Wolfgang and Zeillinger, Robert and Krainer, Michael},
   article_location = {Athens},
   article_number = {4},
   doi = {http://dx.doi.org/10.3892/ijo.2013.1824},
   keywords = {TUSC3; ovarian cancer; glycosylation; proliferation},
   language = {eng},
   issn = {1019-6439},
   journal = {International Journal of Oncology},
   title = {Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells},
   volume = {42},
   year = {2013}
}

TY  - JOUR
ID  - 1086737
AU  - Vaňhara, Petr - Horak, Peter - Pils, Dietmar - Anees, Mariam - Petz, Michaela - Gregor, Wolfgang - Zeillinger, Robert - Krainer, Michael
PY  - 2013
TI  - Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells
JF  - International Journal of Oncology
VL  - 42
IS  - 4
SP  - 1383-1389
EP  - 1383-1389
PB  - SPANDIDOS PUBL LTD
SN  - 10196439
KW  - TUSC3
KW  - ovarian cancer
KW  - glycosylation
KW  - proliferation
N2  - Consequences of deregulated protein N-glycosylation on cancer pathogenesis are poorly understood. TUSC3 is a gene with a putative function in N-glycosylation, located on the short arm of chromosome 8. This is a chromosomal region of frequent genetic loss in ovarian cancer. We established recently that the expression of TUSC3 is epigenetically decreased in epithelial ovarian cancer compared to benign controls and provides prognostic information on patient survival. Therefore, we analyzed the consequences of silenced TUSC3 expression on proliferation, invasion and migration of ovarian cell lines. In addition, we performed subcellular fractionation, co-immunofluorescence and co-immunoprecipitation experiments to establish the molecular localization of TUSC3 in ovarian cancer cells. We demonstrated that TUSC3 is localized in the endoplasmic reticulum as a subunit of the oligosaccharyltransferase complex and is capable of modulation of glycosylation patterning of ovarian cancer cells. Most importantly, silencing of TUSC3 enhances proliferation and migration of ovarian cancer cells in vitro. Our observations suggest a role for N-glycosylating events in ovarian cancer pathogenesis in general, and identify TUSC3 as a tumor suppressor gene in ovarian cancer in particular.
ER  -

VAŇHARA, Petr, Peter HORAK, Dietmar PILS, Mariam ANEES, Michaela PETZ, Wolfgang GREGOR, Robert ZEILLINGER a Michael KRAINER. Loss of the oligosaccharyl transferase subunit TUSC3 promotes proliferation and migration of ovarian cancer cells. \textit{International Journal of Oncology}. Athens: SPANDIDOS PUBL LTD, 2013, roč.~42, č.~4, s.~1383-1389. ISSN~1019-6439. Dostupné z: https://dx.doi.org/10.3892/ijo.2013.1824.

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