Effect of zinc(II) ions on the expression of pro and
anti-apoptotic factors in high-grade prostate carcinoma cells

a 2012

Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells

BALVAN, Jan; Monika HOLUBOVÁ; Jaromír GUMULEC; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ et. al.

Basic information

Original name

Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells

Authors

BALVAN, Jan; Monika HOLUBOVÁ; Jaromír GUMULEC; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ; Vojtěch ADAM; René KIZEK and Michal MASAŘÍK

Edition

MendelNet 2012, 2012

Other information

Language

Czech

Type of outcome

Conference abstract

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Keywords in English

apoptosis;prostate cancer;prognostic marker;Bcl-2;p53;polymerase chain reaction,thiol group;fluorescence microscopy

Changed: 14/2/2013 14:53, doc. MUDr. Jaromír Gumulec, Ph.D.

Abstract

In the original language

Several typical characteristics of prostate tissue have been identified including the ability to accumulate zinc(II). However, this feature of prostate cells is lost during carcinogenesis and, thus, prostate cells are unable to accumulate zinc(II) ions in high levels. Therefore, we can expect that zinc(II) ions can significantly contribute to the progression of tumour disease and to the ability of prostate cell lines to metastasize. In this study, we aimed our attention on determining the expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-kB and p53 genes in two prostate cell lines, as the 22Rv1 cell line, a model of aggressive partially androgen-sensitive prostate cancer and the PNT1A cell line, a normal prostate cell line model. Moreover, we were interested in the mechanisms through which exposure of these cell lines to zinc(II) ions could influence expression of the above-mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation, extremely low expression of p53, high expression of Bcl-2 and no changes in the expression of p53. Our experimental data show different effect of zinc(II) ions on expression of the above-mentioned regulatory genes, which may give us more information on their impact on cancer development and progression with possible using for cancer therapy.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GAP301/10/0356, research and development project

Name: Studie participace specifických mechanismů poškození DNA na cytoxicitě cytostatik vůči lidským chemosensitivním a chemorestentním neuroblastomům

Cite

BALVAN, Jan; Monika HOLUBOVÁ; Jaromír GUMULEC; Markéta SZTALMACHOVÁ; Martina RAUDENSKÁ; Vojtěch ADAM; René KIZEK and Michal MASAŘÍK. Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells. In MendelNet 2012. 2012.

@proceedings{1087010,
   author = {Balvan, Jan and Holubová, Monika and Gumulec, Jaromír and Sztalmachová, Markéta and Raudenská, Martina and Adam, Vojtěch and Kizek, René and Masařík, Michal},
   booktitle = {MendelNet 2012},
   keywords = {apoptosis;prostate cancer;prognostic marker;Bcl-2;p53;polymerase chain reaction,thiol group;fluorescence microscopy},
   language = {cze},
   title = {Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells},
   url = {http://mnet.mendelu.cz/mendelnet2012/articles/39_balvan_661.pdf},
   year = {2012}
}

TY  - CONF
ID  - 1087010
AU  - Balvan, Jan - Holubová, Monika - Gumulec, Jaromír - Sztalmachová, Markéta - Raudenská, Martina - Adam, Vojtěch - Kizek, René - Masařík, Michal
PY  - 2012
TI  - Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells
KW  - apoptosis;prostate cancer;prognostic marker;Bcl-2;p53;polymerase chain reaction,thiol group;fluorescence microscopy
UR  - http://mnet.mendelu.cz/mendelnet2012/articles/39_balvan_661.pdf
N2  - Several typical characteristics of prostate tissue have been identified including the ability to accumulate zinc(II). However, this feature of prostate cells is lost during carcinogenesis and, thus, prostate cells are unable to accumulate zinc(II) ions in high levels. Therefore, we can expect that zinc(II) ions can significantly contribute to the progression of tumour disease and to the ability of prostate cell lines to metastasize. In this study, we aimed our attention on determining the expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-kB and p53 genes in two prostate cell lines, as the 22Rv1 cell line, a model of aggressive partially androgen-sensitive prostate cancer and the PNT1A cell line, a normal prostate cell line model. Moreover, we were interested in the mechanisms through which exposure of these cell lines to zinc(II) ions could influence expression of the above-mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation, extremely low expression of p53, high expression of Bcl-2 and no changes in the expression of p53. Our experimental data show different effect of zinc(II) ions on expression of the above-mentioned regulatory genes, which may give us more information on their impact on cancer development and progression with possible using for cancer therapy.
ER  -

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