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@inbook{1088283,
author = {Řemínek, Roman and Pauwels, Jochen and Wang, Xu and Hoogmartens, Jos and Glatz, Zdeněk and Van Schepdael, Ann},
address = {Hoboken, New Jersey},
booktitle = {Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations},
doi = {http://dx.doi.org/10.1002/9781118530009.ch17},
edition = {1.},
editor = {Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho},
keywords = {Capillary electrophoresis; human liver microsomes; cytochrome P450 enzyme; drug development; metabolic stability;},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Hoboken, New Jersey},
isbn = {978-0-470-57217-7},
pages = {309-317},
publisher = {John Wiley & Sons, Inc.},
title = {Application of capillary electrophoresis for high-throughput screening of drug metabolism},
url = {http://eu.wiley.com/WileyCDA/WileyTitle/productCd-0470572175,descCd-description.html},
year = {2013}
}
TY - CHAP
ID - 1088283
AU - Řemínek, Roman - Pauwels, Jochen - Wang, Xu - Hoogmartens, Jos - Glatz, Zdeněk - Van Schepdael, Ann
PY - 2013
TI - Application of capillary electrophoresis for high-throughput screening of drug metabolism
VL - Neuveden
PB - John Wiley & Sons, Inc.
CY - Hoboken, New Jersey
SN - 9780470572177
KW - Capillary electrophoresis
KW - human liver microsomes
KW - cytochrome P450 enzyme
KW - drug development
KW - metabolic stability;
UR - http://eu.wiley.com/WileyCDA/WileyTitle/productCd-0470572175,descCd-description.html
N2 - Pharmaceutical industry currently needs to screen extensive compound libraries within pre-clinical stages of drug development. Capillary electrophoresis has become a well-established analytical technique during the last two decades. In spite of the fact that capillary electrophoresis offers a number of favorable features for high-throughput analytical assays, it is still less important than chromatographic techniques in drug development programs. Hence, the main aim of this chapter is to discuss the practical limitations of capillary electrophoresis utilization and suggest ways to overcome these constraints. This is illustrated with a practical example namely the introduction of a method allowing rapid characterization of a compound’s stability against human liver microsomes. NADP+ production monitoring was adopted to ensure generic applicability of the method. The fast stability screening of 12 chosen probe drugs and determination of apparent kinetic parameters for the lidocaine/human liver microsomes system were performed in order to demonstrate the potential of the final method. The results were in a good agreement with the literature data determined by different methods.
ER -
ŘEMÍNEK, Roman, Jochen PAUWELS, Xu WANG, Jos HOOGMARTENS, Zdeněk GLATZ a Ann VAN SCHEPDAEL. Application of capillary electrophoresis for high-throughput screening of drug metabolism. In Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho. \textit{Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations}. 1. vyd. Hoboken, New Jersey: John Wiley \&{} Sons, Inc., 2013, s.~309-317. ISBN~978-0-470-57217-7. Dostupné z: https://dx.doi.org/10.1002/9781118530009.ch17.
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