Experimental treatment of deep subchondral articular surface
defect of the femur

a 2012

Experimental treatment of deep subchondral articular surface defect of the femur

PLÁNKA, Ladislav, Robert SRNEC, David STARÝ, Lucy VOJTOVÁ, Petr GÁL et. al.

Základní údaje

Originální název

Experimental treatment of deep subchondral articular surface defect of the femur

Název česky

Experimentální léčba subchondrálního defektu kloubního povrchu stehenní kosti

Autoři

PLÁNKA, Ladislav, Robert SRNEC, David STARÝ, Lucy VOJTOVÁ, Petr GÁL, Jiří KLÍMA a Alois NEČAS

Vydání

Journal of Children´s Orthopaedics : Abstracts for 31st EPOS Annual Meeting Helsinki, 18-21 April, 2012, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30211 Orthopaedics

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Lékařská fakulta

ISSN

Klíčová slova anglicky

Femur; Joint, Cartilage, MSCs

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 4. 2013 14:45, Soňa Böhmová

Anotace

V originále

Articular cartilage is a tissue with very low regenration ability, therefore the new treatment methods of injuries in this location are constantly sought. Improved healing shows the leasions with current violation of the subchondral bone. Therefore, many treatment methods purposefully violate the subchondral bone, and allowing travel progenitor cells from bone marrow into the defect and ensure at least partial healing (microfracture, forrage). Method This study describes the results of experimental treatment of deep subchondral articular surface defect of the femur (the OCD leason is simulated) with chitosan nanofibers scaffold transplantation staffed mesenchymal stem cells and chondrocytes mixture. Experimental animals were twenty young miniature pigs, the frequency of monitoring 20 weeks. After this period knee arthroscopy evaluation was performed and after the protocol culling of the animal followed the histolgical examination of the healed lesions (O'Driscoll score, Collagen II type, Imunofluorescence - CMDiI). The control group was the same defect created by the stress area of the articular surface of the distal epiphysis of the femur on the other leg, left without treatment. Results Average O'Driscoll score of experimental group A was 15,7 +/- 2,8, in comparison with control group B - O'Driscoll score in average was 4,8 +/- 3,1. In group A higher positivity of Collagen II type (85%) than in group B (25%) was observed as well as in Imunofluorescence (A 80%, B 0%)

Citovat

PLÁNKA, Ladislav, Robert SRNEC, David STARÝ, Lucy VOJTOVÁ, Petr GÁL, Jiří KLÍMA a Alois NEČAS. Experimental treatment of deep subchondral articular surface defect of the femur. In Journal of Children´s Orthopaedics : Abstracts for 31st EPOS Annual Meeting Helsinki, 18-21 April, 2012. 2012. ISSN 1863-2521.

@proceedings{1091903,
   author = {Plánka, Ladislav and Srnec, Robert and Starý, David and Vojtová, Lucy and Gál, Petr and Klíma, Jiří and Nečas, Alois},
   booktitle = {Journal of Children´s Orthopaedics : Abstracts for 31st EPOS Annual Meeting Helsinki, 18-21 April, 2012},
   keywords = {Femur; Joint, Cartilage, MSCs},
   language = {eng},
   title = {Experimental treatment of deep subchondral articular surface defect of the femur},
   url = {http://link.springer.com/content/pdf/10.1007%2Fs11832-012-0390-6.pdf},
   year = {2012}
}

TY  - CONF
ID  - 1091903
AU  - Plánka, Ladislav - Srnec, Robert - Starý, David - Vojtová, Lucy - Gál, Petr - Klíma, Jiří - Nečas, Alois
PY  - 2012
TI  - Experimental treatment of deep subchondral articular surface defect of the femur
KW  - Femur
KW  - Joint, Cartilage, MSCs
UR  - http://link.springer.com/content/pdf/10.1007%2Fs11832-012-0390-6.pdf
N2  - Articular cartilage is a tissue with very low regenration ability, therefore the new treatment methods of injuries in this location are constantly sought. Improved healing shows the leasions with current violation of the subchondral bone. Therefore, many treatment methods purposefully violate the subchondral bone, and allowing travel progenitor cells from bone marrow into the defect and ensure at least partial healing (microfracture, forrage). Method This study describes the results of experimental treatment of deep subchondral articular surface defect of the femur (the OCD leason is simulated) with chitosan nanofibers scaffold transplantation staffed mesenchymal stem cells and chondrocytes mixture. Experimental animals were twenty young miniature pigs, the frequency of monitoring 20 weeks. After this period knee arthroscopy evaluation was performed and after the protocol culling of the animal followed the histolgical examination of the healed lesions (O'Driscoll score, Collagen II type, Imunofluorescence - CMDiI). The control group was the same defect created by the stress area of the articular surface of the distal epiphysis of the femur on the other leg, left without treatment. Results Average O'Driscoll score of experimental group A was 15,7 +/- 2,8, in comparison with control group B - O'Driscoll score in average was 4,8 +/- 3,1. In group A higher positivity of Collagen II type (85%) than in group B (25%) was observed as well as in Imunofluorescence (A 80%, B 0%)
ER  -

PLÁNKA, Ladislav, Robert SRNEC, David STARÝ, Lucy VOJTOVÁ, Petr GÁL, Jiří KLÍMA a Alois NEČAS. Experimental treatment of deep subchondral articular surface defect of the femur. In \textit{Journal of Children´s Orthopaedics : Abstracts for 31st EPOS Annual Meeting Helsinki, 18-21 April, 2012}. 2012. ISSN~1863-2521.

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