Classification of Pseudomonas spp. isolated from Antarctic
samples

k 2012

Classification of Pseudomonas spp. isolated from Antarctic samples

KOSINA, Marcel, Miloš BARTÁK, Ivana MAŠLAŇOVÁ, Andrea PASCUTTI, Ondrej ŠEDO et. al.

Základní údaje

Originální název

Classification of Pseudomonas spp. isolated from Antarctic samples

Název anglicky

Classification of Pseudomonas spp. isolated from Antarctic samples

Autoři

KOSINA, Marcel, Miloš BARTÁK, Ivana MAŠLAŇOVÁ, Andrea PASCUTTI, Ondrej ŠEDO, Matej LEXA a Ivo SEDLÁČEK

Vydání

Planet Under Pressure, 2012

Další údaje

Typ výsledku

Prezentace na konferencích

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Klíčová slova anglicky

bacteria, Pseudomonas, Antarctica, genotype

Změněno: 6. 4. 2013 19:57, Mgr. Marcel Kosina, Ph.D.

Anotace

V originále

It is well established that several bacterial species inhabit extremely cold environments. Our study was focused on Pseudomonas bacteria isolated from numerous samples of rock particles, small stones, soil crusts and surface layers collected in deglaciated terrestrial ecosystems of the Northern part of James Ross Island, Antarctica (63 48 02 S, 57 52 57 W) within the period of 2007-2010. Typical sites of sample collection were shaded from direct sunshine by large stones or rock walls. Microhabitats were either vegetation free or with sparse moss and lichen vegetation. Genotypic and phenotypic tools were used to characterize overall 146 isolated fluorescent strains of Gram negative rods. Genotypic methods comprised of manual ribotyping with HindIII endonuclease, followed by multilocus sequence analyses of rrs, rpoB and rpoD genes. The analyses of phenotype involved extensive biotyping, protein profiling using SDS-PAGE, and MALDI-TOF MS. Some isolates formed separate clusters in protein and ribotype fingerprintings which were represented by the strains of different origin. We confirmed the presence of identical or closely related strains of the same Pseudomonas species isolated from different samples. Our recent studies have led to characterize a few of novel species (e.g. „Pseudomonas prosekii"). Moreover, the results of ribotyping indicated probably more novel species among isolated fluorescent strains. Therefore, further analyses will be carried out to characterize bacterial diversity of this psychrotrophic pseudomonads from various Antarctic samples. This work was partly supported by projects from the Ministry of Education, Youth and Sports of the Czech Republic (MSM0021622415, MSM0021622416 and LM2010009).

Návaznosti

LM2010009, projekt VaV

Název: Projekt CzechPolar - České polární stanice: Stavba a operační náklady

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar - České polární stanice: Stavba a operační náklady

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

KOSINA, Marcel, Miloš BARTÁK, Ivana MAŠLAŇOVÁ, Andrea PASCUTTI, Ondrej ŠEDO, Matej LEXA a Ivo SEDLÁČEK. Classification of Pseudomonas spp. isolated from Antarctic samples. In Planet Under Pressure. 2012.

@proceedings{1094937,
   author = {Kosina, Marcel and Barták, Miloš and Mašlaňová, Ivana and Pascutti, Andrea and Šedo, Ondrej and Lexa, Matej and Sedláček, Ivo},
   booktitle = {Planet Under Pressure},
   keywords = {bacteria, Pseudomonas, Antarctica, genotype},
   title = {Classification of Pseudomonas spp. isolated from Antarctic samples},
   url = {http://elsevier.conference-services.net/resources/247/2808/books/Life%20in%20extreme%20environments.pdf},
   year = {2012}
}

TY  - CONF
ID  - 1094937
AU  - Kosina, Marcel - Barták, Miloš - Mašlaňová, Ivana - Pascutti, Andrea - Šedo, Ondrej - Lexa, Matej - Sedláček, Ivo
PY  - 2012
TI  - Classification of Pseudomonas spp. isolated from Antarctic samples
KW  - bacteria, Pseudomonas, Antarctica, genotype
UR  - http://elsevier.conference-services.net/resources/247/2808/books/Life%20in%20extreme%20environments.pdf
N2  - It is well established that several bacterial species inhabit extremely cold environments. Our study was focused on Pseudomonas bacteria isolated from numerous samples of rock particles, small stones, soil crusts and surface layers collected in deglaciated terrestrial ecosystems of the Northern part of James Ross Island, Antarctica (63 48 02 S, 57 52 57 W) within the period of 2007-2010. Typical sites of sample collection were shaded from direct sunshine by large stones or rock walls. Microhabitats were either vegetation free or with sparse moss and lichen vegetation. Genotypic and phenotypic tools were used to characterize overall 146 isolated fluorescent strains of Gram negative rods. Genotypic methods comprised of manual ribotyping with HindIII endonuclease, followed by multilocus sequence analyses of rrs, rpoB and rpoD genes. The analyses of phenotype involved extensive biotyping, protein profiling using SDS-PAGE, and MALDI-TOF MS. Some isolates formed separate clusters in protein and ribotype fingerprintings which were represented by the strains of different origin. We confirmed the presence of identical or closely related strains of the same Pseudomonas species isolated from different samples. Our recent studies have led to characterize a few of novel species (e.g. „Pseudomonas prosekii"). Moreover, the results of ribotyping indicated probably more novel species among isolated fluorescent strains. Therefore, further analyses will be carried out to characterize bacterial diversity of this psychrotrophic pseudomonads from various Antarctic samples. This work was partly supported by projects from the Ministry of Education, Youth and Sports of the Czech Republic (MSM0021622415, MSM0021622416 and LM2010009).
ER  -

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