Differential effects of insulin and dexamethasone on pulmonary
surfactant-associated genes and proteins in A549 and H441 cells
and lung tissue

J 2013

Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue

RUČKA, Zdeněk, Petr VAŇHARA, Irena KRONTORÁD KOUTNÁ, Lenka TESAŘOVÁ, Michaela POTĚŠILOVÁ et. al.

Základní údaje

Originální název

Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue

Autoři

RUČKA, Zdeněk (203 Česká republika, domácí), Petr VAŇHARA (203 Česká republika, domácí), Irena KRONTORÁD KOUTNÁ (203 Česká republika, garant, domácí), Lenka TESAŘOVÁ (203 Česká republika, domácí), Michaela POTĚŠILOVÁ (203 Česká republika, domácí), Stanislav STEJSKAL (203 Česká republika, domácí), Pavel ŠIMARA (203 Česká republika, domácí), Jan DOLEŽEL (203 Česká republika), Václav ZVONÍČEK (203 Česká republika), Oldřich COUFAL (203 Česká republika) a Ivan ČAPOV (203 Česká republika)

Vydání

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, UNITED STATES, SPANDIDOS PUBL LTD, 2013, 1107-3756

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

PubMed

Impakt faktor

Impact factor: 1.880

Kód RIV

RIV/00216224:14330/13:00066090

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.3892/ijmm.2013.1363

UT WoS

000321079400027

Klíčová slova anglicky

insulin; dexamethasone; surfactant; lung carcinoma; A549; H441

Štítky

cbia-web

Změněno: 2. 3. 2018 10:09, Mgr. Pavel Šimara, Ph.D.

Anotace

V originále

In this study, the effects of insulin and dexamethasone on the expression and mRNA transcription of 4 pulmonary surfactant-associated proteins [surfactant protein (SFTP)A, SFTPB, SFTPC and SFTPD] were examined. The commercially available cell lines, A549 and H441, were used as acceptable models of lung surfactant-producing cells. Subsequently, the effects of insulin on the expression of surfactant-associated proteins were examined in patients with lung adenocarcinoma during lung resection. Our results demonstrated the inhibitory effects of insulin on the transcription of the SFTPB, SFTPC and SFTPD genes in H441 cells and the SFTPB gene in A549 cells. Treatment with insulin significantly decreased the protein expression of SFTPA1 and SFTPA2 in the H441 cells and that of proSFTPB in the A549 cells. Dexamethasone promoted the transcription of the SFTPB, SFTPC and SFTPD genes in the A549 and H441 cells and reduced the transcription of the SFTPA1 and SFTPA2 genes in the H441 cells (SFTPA mRNA expression was not detected in A549 cells). Furthermore, we demonstrated that the mRNA levels of the selected genes were significantly lower in the cell lines compared to the lung tissue. A549 and H441 cells represent similar cell types. Yet, in our experiments, these cells reacted differently to insulin and/or dexamethasone treatment, and the mRNA levels of their main protein products, surfactant-associated proteins,were significantly lower than those in real tissue. Therefore, the results obtained in this study challenge the suitability of A549 and H441 cells as models of type II pneumocytes and Clara cells, respectively. However, we successfully demonstrate the possibility of studying the effects of insulin on pulmonary surfactant-associated genes and proteins in patients with lung adenocarcinoma.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

MSM0021622430, záměr

Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Citovat

RUČKA, Zdeněk, Petr VAŇHARA, Irena KRONTORÁD KOUTNÁ, Lenka TESAŘOVÁ, Michaela POTĚŠILOVÁ, Stanislav STEJSKAL, Pavel ŠIMARA, Jan DOLEŽEL, Václav ZVONÍČEK, Oldřich COUFAL a Ivan ČAPOV. Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE. UNITED STATES: SPANDIDOS PUBL LTD, 2013, roč. 32, č. 1, s. 211-218. ISSN 1107-3756. Dostupné z: https://dx.doi.org/10.3892/ijmm.2013.1363.

@article{1102512,
   author = {Ručka, Zdeněk and Vaňhara, Petr and Krontorád Koutná, Irena and Tesařová, Lenka and Potěšilová, Michaela and Stejskal, Stanislav and Šimara, Pavel and Doležel, Jan and Zvoníček, Václav and Coufal, Oldřich and Čapov, Ivan},
   article_location = {UNITED STATES},
   article_number = {1},
   doi = {http://dx.doi.org/10.3892/ijmm.2013.1363},
   keywords = {insulin; dexamethasone; surfactant; lung carcinoma; A549; H441},
   language = {eng},
   issn = {1107-3756},
   journal = {INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE},
   title = {Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/23620014},
   volume = {32},
   year = {2013}
}

TY  - JOUR
ID  - 1102512
AU  - Ručka, Zdeněk - Vaňhara, Petr - Krontorád Koutná, Irena - Tesařová, Lenka - Potěšilová, Michaela - Stejskal, Stanislav - Šimara, Pavel - Doležel, Jan - Zvoníček, Václav - Coufal, Oldřich - Čapov, Ivan
PY  - 2013
TI  - Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue
JF  - INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
VL  - 32
IS  - 1
SP  - 211-218
EP  - 211-218
PB  - SPANDIDOS PUBL LTD
SN  - 11073756
KW  - insulin
KW  - dexamethasone
KW  - surfactant
KW  - lung carcinoma
KW  - A549
KW  - H441
UR  - http://www.ncbi.nlm.nih.gov/pubmed/23620014
L2  - http://www.ncbi.nlm.nih.gov/pubmed/23620014
N2  - In this study, the effects of insulin and dexamethasone on the expression and mRNA transcription of 4 pulmonary surfactant-associated proteins [surfactant protein (SFTP)A, SFTPB, SFTPC and SFTPD] were examined. The commercially available cell lines, A549 and H441, were used as acceptable models of lung surfactant-producing cells. Subsequently, the effects of insulin on the expression of surfactant-associated proteins were examined in patients with lung adenocarcinoma during lung resection. Our results demonstrated the inhibitory effects of insulin on the transcription of the SFTPB, SFTPC and SFTPD genes in H441 cells and the SFTPB gene in A549 cells. Treatment with insulin significantly decreased the protein expression of SFTPA1 and SFTPA2 in the H441 cells and that of proSFTPB in the A549 cells. Dexamethasone promoted the transcription of the SFTPB, SFTPC and SFTPD genes in the A549 and H441 cells and reduced the transcription of the SFTPA1 and SFTPA2 genes in the H441 cells (SFTPA mRNA expression was not detected in A549 cells). Furthermore, we demonstrated that the mRNA levels of the selected genes were significantly lower in the cell lines compared to the lung tissue. A549 and H441 cells represent similar cell types. Yet, in our experiments, these cells reacted differently to insulin and/or dexamethasone treatment, and the mRNA levels of their main protein products, surfactant-associated proteins,were significantly lower than those in real tissue. Therefore, the results obtained in this study challenge the suitability of A549 and H441 cells as models of type II pneumocytes and Clara cells, respectively. However, we successfully demonstrate the possibility of studying the effects of insulin on pulmonary surfactant-associated genes and proteins in patients with lung adenocarcinoma.
ER  -

RUČKA, Zdeněk, Petr VAŇHARA, Irena KRONTORÁD KOUTNÁ, Lenka TESAŘOVÁ, Michaela POTĚŠILOVÁ, Stanislav STEJSKAL, Pavel ŠIMARA, Jan DOLEŽEL, Václav ZVONÍČEK, Oldřich COUFAL a Ivan ČAPOV. Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue. \textit{INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE}. UNITED STATES: SPANDIDOS PUBL LTD, 2013, roč.~32, č.~1, s.~211-218. ISSN~1107-3756. Dostupné z: https://dx.doi.org/10.3892/ijmm.2013.1363.

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