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@article{1102518,
author = {Faltejsková, Petra and Bočánek, Ondřej and Šachlová, Milana and Svoboda, Marek and Kiss, Igor and Vyzula, Rostislav and Slabý, Ondřej},
article_location = {Amsterdam},
article_number = {4-5},
doi = {http://dx.doi.org/10.3233/CBM-130308},
keywords = {microRNAs; serum; colorectal cancer; biomarkers},
language = {eng},
issn = {1574-0153},
journal = {Cancer Biomarkers},
title = {Circulating miR-17-3p, miR-29a, miR-92a and miR-135b in serum: Evidence against their usage as biomarkers in colorectal cancer},
volume = {12},
year = {2012}
}
TY - JOUR
ID - 1102518
AU - Faltejsková, Petra - Bočánek, Ondřej - Šachlová, Milana - Svoboda, Marek - Kiss, Igor - Vyzula, Rostislav - Slabý, Ondřej
PY - 2012
TI - Circulating miR-17-3p, miR-29a, miR-92a and miR-135b in serum: Evidence against their usage as biomarkers in colorectal cancer
JF - Cancer Biomarkers
VL - 12
IS - 4-5
SP - 199-204
EP - 199-204
PB - IOS Press
SN - 15740153
KW - microRNAs
KW - serum
KW - colorectal cancer
KW - biomarkers
N2 - BACKGROUND: Colorectal cancer (CRC) is one of the leading causes of cancer-related death in the world. Therefore, there is a high demand for cost-effective and non-invasive biomarkers that would enable an early detection of asymptomatic and curable disease with high sensitivity and specificity. OBJECTIVE: The main objective of this study was to investigate the potential of circulating miRNAs as biomarkers of CRC. METHODS: Total RNA enriched for small RNAs was isolated from 100~sera of patients with CRC and 30 sera of healthy donors. The expression levels of miR-17-3p, miR-29a, miR-92a and miR-135b were determined using quantitative real-time PCR. The average expression levels of particular miRNAs were normalized to miR-16 levels and statistically evaluated. RESULTS: Using Mann-Whitney U test, no significant differences were observed in miR-17-3p (P=0.18), miR-29a (P=0.14) and miR-92a (P=0.60) levels between sera of CRC patients and controls. The levels of miR-135b in serum were too low to be quantified accurately. Subsequently, we tried to correlate expression levels of analyzed miRNAs to clinical-pathological features of CRC patients. Only levels of mir-29a were correlated with the clinical stage (P=0.04). Expression levels of the other miRNAs were correlated neither with the clinical stage, nor with the grade. CONCLUSIONS: Interestingly, our results are contradictory to previous studies performed on the CRC patients from Chinese population, providing an evidence against usage of serum miR-17-3p, miR-29a, miR-92a and miR-135b as new biomarkers for early detection of CRC.
ER -
FALTEJSKOVÁ, Petra, Ondřej BOČÁNEK, Milana ŠACHLOVÁ, Marek SVOBODA, Igor KISS, Rostislav VYZULA and Ondřej SLABÝ. Circulating miR-17-3p, miR-29a, miR-92a and miR-135b in serum: Evidence against their usage as biomarkers in colorectal cancer. \textit{Cancer Biomarkers}. Amsterdam: IOS Press, 2012, vol.~12, 4-5, p.~199-204. ISSN~1574-0153. Available from: https://dx.doi.org/10.3233/CBM-130308.
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