J 2013

Identification of MicroRNAs Regulated by Isothiocyanates and Association of Polymorphisms Inside Their Target Sites with Risk of Sporadic Colorectal Cancer

SLABÝ, Ondřej, Milana ŠACHLOVÁ, Veronika BŘEZKOVÁ, Renata HÉŽOVÁ, Alena KOVAŘÍKOVÁ et. al.

Basic information

Original name

Identification of MicroRNAs Regulated by Isothiocyanates and Association of Polymorphisms Inside Their Target Sites with Risk of Sporadic Colorectal Cancer

Authors

SLABÝ, Ondřej (203 Czech Republic, guarantor, belonging to the institution), Milana ŠACHLOVÁ (203 Czech Republic), Veronika BŘEZKOVÁ (203 Czech Republic, belonging to the institution), Renata HÉŽOVÁ (203 Czech Republic, belonging to the institution), Alena KOVAŘÍKOVÁ (203 Czech Republic, belonging to the institution), Svatava BISCHOFOVÁ (203 Czech Republic), Sabina ŠEVČÍKOVÁ (203 Czech Republic, belonging to the institution), Julie BIENERTOVÁ VAŠKŮ (203 Czech Republic, belonging to the institution), Anna VAŠKŮ (203 Czech Republic, belonging to the institution), Marek SVOBODA (203 Czech Republic) and Rostislav VYZULA (203 Czech Republic)

Edition

Nutrition and cancer : an international journal, Abingdon, Routledge Journals, Taylor & Francis, 2013, 0163-5581

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.635

RIV identification code

RIV/00216224:14740/13:00068077

Organization unit

Central European Institute of Technology

UT WoS

000315352200010

Keywords in English

microRNAs; colorectal cancer; isothiocyanates; polymorphism

Tags

Tags

International impact, Reviewed
Změněno: 9/4/2014 08:27, Olga Křížová

Abstract

V originále

Sporadic colorectal cancer (CRC) is a typical multifactorial disease. Isothiocyanates (ITC) have been recently shown to inhibit development of CRC in many experimental models. MicroRNAs (miRNAs) are short noncoding RNAs that posttranscriptionally regulate gene expression through binding to 3 untranslated regions of target mRNAs. MiRNAs are regulated by natural agents, ITCs included. In our study, using global expression profiling based on TaqMan Low-Density Arrays, we identified 3 common miRNAs (miR-155, miR-23b, miR-27b) regulated by ITCs (sulforaphane, iberin) in colonic epithelial cell lines NCM460 and NCM356. In silico predictions allowed us to find 9 relevant single nucleotide polymorphisms (SNPs) localized within the 3UTRs of genes (AGTR1, TNFAIP2, PRKCB, HSPA9, RABGAP1, DICER1, ADAM19, VWA5A, and SIRT5) targeted by these ITC-related miRNAs. Finally, we observed that homozygous CC genotype of DICER1, rs1057035, was significantly associated with decreased risk of CRC (odds ratio = 0.49; 95% confidence interval: 0.25–0.95, P = 0.036) when compared to TT homozygote genotype; also, the C allele tended to have a protective effect (P = 0.072). This study showed that miRNAs could be involved in chemoprotective effects of natural agents; their function alteration through SNPs in their binding sites and flanking regions presents a new class of CRC risk factors.

Links

ED1.1.00/02.0068, research and development project
Name: CEITEC - central european institute of technology