| FALTEJSKOVÁ, Petra, Marek SVOBODA, Jitka MLČOCHOVÁ, Klára ŠRŮTOVÁ, Pavel FABIAN, Rostislav VYZULA a Ondřej SLABÝ. MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer. In Annual Meeting 2013, April 6-10, 2013, Walter E. Washington Convention Center, Washington, DC. 2013. ISSN 0008-5472. |
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@proceedings{1103860,
author = {Faltejsková, Petra and Svoboda, Marek and Mlčochová, Jitka and Šrůtová, Klára and Fabian, Pavel and Vyzula, Rostislav and Slabý, Ondřej},
booktitle = {Annual Meeting 2013, April 6-10, 2013, Walter E. Washington Convention Center, Washington, DC},
keywords = {microRNAs; colorectal cancer; in vitro analysis; miR-215},
language = {eng},
title = {MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer},
year = {2013}
}
TY - CONF
ID - 1103860
AU - Faltejsková, Petra - Svoboda, Marek - Mlčochová, Jitka - Šrůtová, Klára - Fabian, Pavel - Vyzula, Rostislav - Slabý, Ondřej
PY - 2013
TI - MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer
KW - microRNAs
KW - colorectal cancer
KW - in vitro analysis
KW - miR-215
N2 - Background: MicroRNAs (miRNAs) constitute a robust regulatory network with post-transcriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumor suppressors. Methods: We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoral tissues and identified 42 differentially expressed miRNAs. One of the most significantly altered miRNAs was miR-215, significantly down-regulated in tumor tissue. We chose miR-215 for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro functional studies on DLD1, HCT116 and HT29 cell lines. Results: MiR-215 was proved to be significantly decreased in CRC tumor tissues (p<0.0001) and to be negatively correlated with clinical stage (p<0.0001) and tumor grade (p=0.04). In vitro analyses showed that ectopic expression of miR 215 decreases viability in DLD1 (p=0.05) and HCT116 (p=0,05) cells, increases apoptosis in HCT116 (p=0.005), promotes cell cycle arrest (inhibited G1/S transition) in HCT-116 colon cancer cells (p=0.01) and decreases migration in DLD1 (p=0.05) and HT-29 cells (p=0.05). We further confirmed transcription factor HOXB9 as direct target of miR-215 on the mRNA and protein level. Conclusions: These findings indicate that miR 215 play an important role in CRC as tumor suppressor and contributes to CRC pathogenesis.
ER -
FALTEJSKOVÁ, Petra, Marek SVOBODA, Jitka MLČOCHOVÁ, Klára ŠRŮTOVÁ, Pavel FABIAN, Rostislav VYZULA a Ondřej SLABÝ. MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer. In \textit{Annual Meeting 2013, April 6-10, 2013, Walter E. Washington Convention Center, Washington, DC}. 2013. ISSN~0008-5472.
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