MiR-215 expression in tumor tissue and in vitro effects of its
replacement in colorectal cancer

a 2013

MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer

FALTEJSKOVÁ, Petra, Marek SVOBODA, Jitka MLČOCHOVÁ, Klára ŠRŮTOVÁ, Pavel FABIAN et. al.

Základní údaje

Originální název

MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer

Autoři

FALTEJSKOVÁ, Petra (203 Česká republika, domácí), Marek SVOBODA (203 Česká republika), Jitka MLČOCHOVÁ (203 Česká republika, domácí), Klára ŠRŮTOVÁ (203 Česká republika, domácí), Pavel FABIAN (203 Česká republika), Rostislav VYZULA (203 Česká republika) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

Annual Meeting 2013, April 6-10, 2013, Walter E. Washington Convention Center, Washington, DC, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 9.284

Kód RIV

RIV/00216224:14740/13:00065594

Organizační jednotka

Středoevropský technologický institut

ISSN

Klíčová slova anglicky

microRNAs; colorectal cancer; in vitro analysis; miR-215

Příznaky

Mezinárodní význam

Změněno: 22. 10. 2015 19:38, Mgr. Petra Vychytilová, Ph.D.

Anotace

V originále

Background: MicroRNAs (miRNAs) constitute a robust regulatory network with post-transcriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumor suppressors. Methods: We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoral tissues and identified 42 differentially expressed miRNAs. One of the most significantly altered miRNAs was miR-215, significantly down-regulated in tumor tissue. We chose miR-215 for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro functional studies on DLD1, HCT116 and HT29 cell lines. Results: MiR-215 was proved to be significantly decreased in CRC tumor tissues (p<0.0001) and to be negatively correlated with clinical stage (p<0.0001) and tumor grade (p=0.04). In vitro analyses showed that ectopic expression of miR 215 decreases viability in DLD1 (p=0.05) and HCT116 (p=0,05) cells, increases apoptosis in HCT116 (p=0.005), promotes cell cycle arrest (inhibited G1/S transition) in HCT-116 colon cancer cells (p=0.01) and decreases migration in DLD1 (p=0.05) and HT-29 cells (p=0.05). We further confirmed transcription factor HOXB9 as direct target of miR-215 on the mRNA and protein level. Conclusions: These findings indicate that miR 215 play an important role in CRC as tumor suppressor and contributes to CRC pathogenesis.

Návaznosti

NT13549, projekt VaV

Název: Vytvoření diagnostické sady cirkulujících mikroRNA pro neinvazivní časnou diagnostiku a sledování pacientů s kolorektálním karcinomem

Citovat

FALTEJSKOVÁ, Petra, Marek SVOBODA, Jitka MLČOCHOVÁ, Klára ŠRŮTOVÁ, Pavel FABIAN, Rostislav VYZULA a Ondřej SLABÝ. MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer. In Annual Meeting 2013, April 6-10, 2013, Walter E. Washington Convention Center, Washington, DC. 2013. ISSN 0008-5472.

@proceedings{1103860,
   author = {Faltejsková, Petra and Svoboda, Marek and Mlčochová, Jitka and Šrůtová, Klára and Fabian, Pavel and Vyzula, Rostislav and Slabý, Ondřej},
   booktitle = {Annual Meeting 2013, April 6-10, 2013, Walter E. Washington Convention Center, Washington, DC},
   keywords = {microRNAs; colorectal cancer; in vitro analysis; miR-215},
   language = {eng},
   title = {MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer},
   year = {2013}
}

TY  - CONF
ID  - 1103860
AU  - Faltejsková, Petra - Svoboda, Marek - Mlčochová, Jitka - Šrůtová, Klára - Fabian, Pavel - Vyzula, Rostislav - Slabý, Ondřej
PY  - 2013
TI  - MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer
KW  - microRNAs
KW  - colorectal cancer
KW  - in vitro analysis
KW  - miR-215
N2  - Background: MicroRNAs (miRNAs) constitute a robust regulatory network with post-transcriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumor suppressors. Methods: We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoral tissues and identified 42 differentially expressed miRNAs. One of the most significantly altered miRNAs was miR-215, significantly down-regulated in tumor tissue. We chose miR-215 for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro functional studies on DLD1, HCT116 and HT29 cell lines. Results: MiR-215 was proved to be significantly decreased in CRC tumor tissues (p<0.0001) and to be negatively correlated with clinical stage (p<0.0001) and tumor grade (p=0.04). In vitro analyses showed that ectopic expression of miR 215 decreases viability in DLD1 (p=0.05) and HCT116 (p=0,05) cells, increases apoptosis in HCT116 (p=0.005), promotes cell cycle arrest (inhibited G1/S transition) in HCT-116 colon cancer cells (p=0.01) and decreases migration in DLD1 (p=0.05) and HT-29 cells (p=0.05). We further confirmed transcription factor HOXB9 as direct target of miR-215 on the mRNA and protein level. Conclusions: These findings indicate that miR 215 play an important role in CRC as tumor suppressor and contributes to CRC pathogenesis.
ER  -

FALTEJSKOVÁ, Petra, Marek SVOBODA, Jitka MLČOCHOVÁ, Klára ŠRŮTOVÁ, Pavel FABIAN, Rostislav VYZULA a Ondřej SLABÝ. MiR-215 expression in tumor tissue and in vitro effects of its replacement in colorectal cancer. In \textit{Annual Meeting 2013, April 6-10, 2013, Walter E. Washington Convention Center, Washington, DC}. 2013. ISSN~0008-5472.

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