Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus
ferrooxidans and analysis of the process at the levels of
transcription and protein synthesis

J 2013

Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis

KUČERA, Jiří; Pavel BOUCHAL; Jan LOCHMAN; David POTĚŠIL; Oldřich JANICZEK et al.

Základní údaje

Originální název

Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis

Název česky

Oxidace železa(II) síru oxidující bakterií Acidithiobacillus ferrooxidans a analýza procesu na úrovni transkripce a syntézy proteinů

Autoři

KUČERA, Jiří; Pavel BOUCHAL; Jan LOCHMAN; David POTĚŠIL; Oldřich JANICZEK; Zbyněk ZDRÁHAL a Martin MANDL

Vydání

Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, Dordrecht, Springer, 2013, 0003-6072

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.137

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/13:00066107

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1007/s10482-012-9872-2

UT WoS

000316145900021

Klíčová slova česky

Acidithiobacillus ferrooxidans; oxidace síry, oxidace železa(II); oxidace pyritu; proteomika, RT PCR

Klíčová slova anglicky

Acidithiobacillus ferrooxidans; elemental sulfur oxidation; ferrous iron oxidation; pyrite oxidation; proteomics; reverse-transcription quantitative PCR

Štítky

AKR, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 1. 2016 14:26, doc. Ing. Martin Mandl, CSc.

Anotace

ORIG CZ

V originále

In contrast to iron-oxidizing Acidithiobacillus ferrooxidans, A. ferrooxidans from a stationary phase elemental sulfur-oxidizing culture exhibited a lag phase in pyrite oxidation, which is similar to its behavior during ferrous iron oxidation. The ability of elemental sulfur-oxidizing A. ferrooxidans to immediately oxidize ferrous iron or pyrite without a lag phase was only observed in bacteria obtained from growing cultures with elemental sulfur. However, these cultures that shifted to ferrous iron oxidation showed a low rate of ferrous iron oxidation while no growth was observed. Two-dimensional gel electrophoresis was used for a quantitative proteomic analysis of the adaptation process when bacteria were switched from elemental sulfur to ferrous iron. A comparison of total cell lysates revealed 39 proteins whose increase or decrease in abundance was related to this phenotypic switching. However, only a few proteins were closely related to iron and sulfur metabolism. Reverse-transcription quantitative PCR was used to further characterize the bacterial adaptation process. The expression profiles of selected genes primarily involved in the ferrous iron oxidation indicated that phenotypic switching is a complex process that includes the activation of genes encoding a membrane protein, maturation proteins, electron transport proteins and their regulators.

Česky

Ve srovnání se železo oxidující bakterií A. ferrooxidans vykazovala síru oxidující bakterie A. ferrooxidans při oxidaci železa(II) a pyritu odlišné biochemické parametry. Adaptace na oxidaci železa(II) byla monitorována kinetickou, proteomickou a transkriptomickou analýzou za účelem diskuse regulace adaptace.

Návaznosti

CZ.1.05/1.1.00/02.0068, interní kód MU

Název: CEITEC - středoevropský technologický institut (Akronym: CEITEC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC - středoevropský technologický institut, 1.1 Evropská centra excelence

GA525/08/0697, projekt VaV

Název: Aktivita acidofilních bakterií oxidujících síru za aerobních a anaerobních podmínek

Investor: Grantová agentura ČR, Aktivita acidofilních bakterií oxidujících síru za aerobních a anaerobních podmínek

Přiložené soubory

ZVV_2013_29_1104551.pdf

Požádat o autorskou verzi souboru

Citovat

KUČERA, Jiří; Pavel BOUCHAL; Jan LOCHMAN; David POTĚŠIL; Oldřich JANICZEK; Zbyněk ZDRÁHAL a Martin MANDL. Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Dordrecht: Springer, 2013, roč. 103, č. 4, s. 905-919. ISSN 0003-6072. Dostupné z: https://doi.org/10.1007/s10482-012-9872-2.

@article{1104551,
   author = {Kučera, Jiří and Bouchal, Pavel and Lochman, Jan and Potěšil, David and Janiczek, Oldřich and Zdráhal, Zbyněk and Mandl, Martin},
   article_location = {Dordrecht},
   article_number = {4},
   doi = {https://doi.org/10.1007/s10482-012-9872-2},
   keywords = {Acidithiobacillus ferrooxidans; elemental sulfur oxidation; ferrous iron oxidation; pyrite oxidation; proteomics; reverse-transcription quantitative PCR},
   language = {eng},
   issn = {0003-6072},
   journal = {Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology},
   title = {Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis},
   volume = {103},
   year = {2013}
}

TY  - JOUR
ID  - 1104551
AU  - Kučera, Jiří - Bouchal, Pavel - Lochman, Jan - Potěšil, David - Janiczek, Oldřich - Zdráhal, Zbyněk - Mandl, Martin
PY  - 2013
TI  - Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis
JF  - Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
VL  - 103
IS  - 4
SP  - 905-919
EP  - 905-919
PB  - Springer
SN  - 00036072
KW  - Acidithiobacillus ferrooxidans
KW  - elemental sulfur oxidation
KW  - ferrous iron oxidation
KW  - pyrite oxidation
KW  - proteomics
KW  - reverse-transcription quantitative PCR
N2  - In contrast to iron-oxidizing Acidithiobacillus ferrooxidans, A. ferrooxidans from a stationary phase elemental sulfur-oxidizing culture exhibited a lag phase in pyrite oxidation, which is similar to its behavior during ferrous iron oxidation. The ability of elemental sulfur-oxidizing A. ferrooxidans to immediately oxidize ferrous iron or pyrite without a lag phase was only observed in bacteria obtained from growing cultures with elemental sulfur. However, these cultures that shifted to ferrous iron oxidation showed a low rate of ferrous iron oxidation while no growth was observed. Two-dimensional gel electrophoresis was used for a quantitative proteomic analysis of the adaptation process when bacteria were switched from elemental sulfur to ferrous iron. A comparison of total cell lysates revealed 39 proteins whose increase or decrease in abundance was related to this phenotypic switching. However, only a few proteins were closely related to iron and sulfur metabolism. Reverse-transcription quantitative PCR was used to further characterize the bacterial adaptation process. The expression profiles of selected genes primarily involved in the ferrous iron oxidation indicated that phenotypic switching is a complex process that includes the activation of genes encoding a membrane protein, maturation proteins, electron transport proteins and their regulators.
ER  -

KUČERA, Jiří; Pavel BOUCHAL; Jan LOCHMAN; David POTĚŠIL; Oldřich JANICZEK; Zbyněk ZDRÁHAL a Martin MANDL. Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis. \textit{Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology}. Dordrecht: Springer, 2013, roč.~103, č.~4, s.~905-919. ISSN~0003-6072. Dostupné z: https://doi.org/10.1007/s10482-012-9872-2.

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