Classification of Aeromonas veronii bv. sobria strains isolated
from human clinical material

a 2013

Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material

ČERNOHLÁVKOVÁ, Jitka, Michaela SEDLÁČKOVÁ, Eva KREJČÍ a Ivo SEDLÁČEK

Základní údaje

Originální název

Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material

Název česky

Klasifikace kmenů Aeromonas veronii bv. sobria izolovaných z humánního klinického materiálu

Autoři

ČERNOHLÁVKOVÁ, Jitka (203 Česká republika, garant, domácí), Michaela SEDLÁČKOVÁ (203 Česká republika), Eva KREJČÍ (203 Česká republika) a Ivo SEDLÁČEK (203 Česká republika)

Vydání

ECCMID 2013, Berlin, Germany, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/13:00068202

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

aeromonády; klasifikace; biotypizace; ribotypizace; sekvencování

Klíčová slova anglicky

Aeromonads; classification; biotyping; ribotyping; gene sequencing

Štítky

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2014 11:04, Ing. Zdeňka Rašková

Anotace

V originále

Aeromonas are gram-negative, oxidase positive, fermenting rods widely distributed in the environment. This genus was recognised as an important fish pathogen, however is often associated with gastrointestinal and extraintestinal diseases in human. Despite its clinical significance Aeromonas spp. are not accurately identified during routine clinical examination, because of their phenotypical similarity. We focused on detailed characterisation of 22 clinical isolates of “A. sobria complex” from diarrhoea samples. Biochemical tests were used to classify isolates into “A. sobria complex”. Further, an approach using ribotyping, whole-cell protein analysis and cpn60 gene sequencing were applied for detailed characterisation and identification. Additionally, 11 reference type strains of Aeromonas from Czech Collection of Microorganisms were included in the comparison. Genetic analysis based on ribotyping clearly distinguished the 22 human isolates from reference Aeromonas type strains and clustered them together with A. veronii bv. sobria, A. veronii bv. veronii and A. allosaccharophila reference strains. A. jandaei and A. schubertii were the next closest relation species. Six isolates were clustered into a separate subclaster(similarity 52%), but results of cpn60 gene sequencing of a selected isolate confirmed A. veronii bv. sobria identification. Whole-cell protein analysis showed different distribution of isolates. 16 isolates were grouped together with A. veronii bv. sobria reference strain (similarity level 48 %) and 8 isolates together with A. veronii bv. veronii reference strain. Other isolates were separated from these groups. Despite inconsistent results of ribotying and whole-cell protein analysis our results showed that studied isolates belonged to “A. sobria complex”, mainly into A. veronii bv. sobria species. Reliable identification of clinical isolates is important for determination of sources of infection.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

Citovat

ČERNOHLÁVKOVÁ, Jitka, Michaela SEDLÁČKOVÁ, Eva KREJČÍ a Ivo SEDLÁČEK. Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material. In ECCMID 2013, Berlin, Germany. 2013.

@proceedings{1104937,
   author = {Černohlávková, Jitka and Sedláčková, Michaela and Krejčí, Eva and Sedláček, Ivo},
   booktitle = {ECCMID 2013, Berlin, Germany},
   keywords = {Aeromonads; classification; biotyping; ribotyping; gene sequencing},
   language = {eng},
   title = {Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material},
   url = {http://www.escmid.org/escmid_library/online_lecture_library/?search=1¤t_page=1&search_term=cernohlavkova},
   year = {2013}
}

TY  - CONF
ID  - 1104937
AU  - Černohlávková, Jitka - Sedláčková, Michaela - Krejčí, Eva - Sedláček, Ivo
PY  - 2013
TI  - Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material
KW  - Aeromonads
KW  - classification
KW  - biotyping
KW  - ribotyping
KW  - gene sequencing
UR  - http://www.escmid.org/escmid_library/online_lecture_library/?search=1¤t_page=1&search_term=cernohlavkova
L2  - http://www.escmid.org/escmid_library/online_lecture_library/?search=1¤t_page=1&search_term=cernohlavkova
N2  - Aeromonas are gram-negative, oxidase positive, fermenting rods widely distributed in the environment. This genus was recognised as an important fish pathogen, however is often associated with gastrointestinal and extraintestinal diseases in human. Despite its clinical significance Aeromonas spp. are not accurately identified during routine clinical examination, because of their phenotypical similarity. We focused on detailed characterisation of 22 clinical isolates of “A. sobria complex” from diarrhoea samples. Biochemical tests were used to classify isolates into “A. sobria complex”. Further, an approach using ribotyping, whole-cell protein analysis and cpn60 gene sequencing were applied for detailed characterisation and identification. Additionally, 11 reference type strains of Aeromonas from Czech Collection of Microorganisms were included in the comparison. Genetic analysis based on ribotyping clearly distinguished the 22 human isolates from reference Aeromonas type strains and clustered them together with A. veronii bv. sobria, A. veronii bv. veronii and A. allosaccharophila reference strains. A. jandaei and A. schubertii were the next closest relation species. Six isolates were clustered into a separate subclaster(similarity 52%), but results of cpn60 gene sequencing of a selected isolate confirmed A. veronii bv. sobria identification. Whole-cell protein analysis showed different distribution of isolates. 16 isolates were grouped together with A. veronii bv. sobria reference strain (similarity level 48 %) and 8 isolates together with A. veronii bv. veronii reference strain. Other isolates were separated from these groups. Despite inconsistent results of ribotying and whole-cell protein analysis our results showed that studied isolates belonged to “A. sobria complex”, mainly into A. veronii bv. sobria species. Reliable identification of clinical isolates is important for determination of sources of infection.
ER  -

ČERNOHLÁVKOVÁ, Jitka, Michaela SEDLÁČKOVÁ, Eva KREJČÍ a Ivo SEDLÁČEK. Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material. In \textit{ECCMID 2013, Berlin, Germany}. 2013.

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