Analysis of B-cells and plasma cells in multiple myeloma

VŠIANSKÁ, Pavla, Lucie ŘÍHOVÁ, Tamara VARMUŽOVÁ, Renata SUSKÁ, Miroslav PENKA and Roman HÁJEK. Analysis of B-cells and plasma cells in multiple myeloma. In 12th Euroconference on CLINICAL CELL ANALYSIS. 2012.

Basic information

• Original name: Analysis of B-cells and plasma cells in multiple myeloma
• Authors: VŠIANSKÁ, Pavla, Lucie ŘÍHOVÁ, Tamara VARMUŽOVÁ, Renata SUSKÁ, Miroslav PENKA and Roman HÁJEK.
• Edition: 12th Euroconference on CLINICAL CELL ANALYSIS, 2012.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Hungary
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• UT WoS: 000310386300071

Abstract

Multiple myeloma (MM) is characterized by the presence of clonal plasma cells (PC) arising from malignant transformed B-cells. It is still not clear which stage of B-cell differentiation is responsible for the development of MM and for eventual relapse after treatment, so nowadays there is an effort to identify the source of myeloma-initiating cells. Aim: Analysis of the phenotypic profile and enumeration of B and PC subpopulations in MM patients. Patients and methods: Total of 38 newly diagnosed MM patients and 18 controls without MM were analysed. Results: There was found significantly lower % of the total CD19+ cells in MM than in control samples for both PB and BM (p <0.05). Numbers of transitional B-cells and naive B-cells were reduced in PB of MM when compared with controls (p <0.05). On the contrary, numbers of preGC, total memory and CD27- switched B-cells were increased in MM (p <0.05). No difference was found when compared % of plasmablasts in both groups. There were found similar results as reduction of naive B-cells and increase of total memory and CD27- switched B-cells in BM of MM (p <0.05). As expected, higher % of PC was found in MM mostly with abnormal phenotype CD19- (p <0.005). Conclusion: Polychromatic flow cytometry is capable to identify minimum of 10 B-cell and PC subpopulations. Reduction of CD19+ cells in MM should be mediated by an increased number of abnormal PCs. The enhancement of isotype-switched memory CD27+ and isotype-switched CD27- B-cell subpopulations in MM patients suggests that some of these could be a potential source of myeloma-initiating cells. Analysis on DNA level will be done.

Links

• GAP304/10/1395, research and development project: Name: Analýza klonálních progenitorů plazmatických buněk u monoklonálních gamapatií

Investor: Czech Science Foundation

• MSM0021622434, plan (intention): Name: Od klasických prognostických markerů ke klinicky aplikovatelným farmakogenomickým a farmakoproteomickým projektům u mnohočetného myelomu a monoklonálních gamapatií

Investor: Ministry of Education, Youth and Sports of the CR, From classic prognostic markers to clinical applications in selected pharmacogenomic and pharmacoproteomic projects in multiple myeloma and monoclonal gammapathies