| PAULOVÁ, Hana, Tibor STRAČINA, Jiří JARKOVSKÝ, Marie NOVÁKOVÁ and Eva TÁBORSKÁ. Hydroxyl radicals’ production and ECG parameters during ischemia and reperfusion in rat, guinea pig and rabbit isolated heart. General Physiology and Biophysics. Bratislava: Veda, 2013, vol. 32, No 2, p. 221-228. ISSN 0231-5882. Available from: https://dx.doi.org/10.4149/gpb_2013016. |
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@article{1112079,
author = {Paulová, Hana and Stračina, Tibor and Jarkovský, Jiří and Nováková, Marie and Táborská, Eva},
article_location = {Bratislava},
article_number = {2},
doi = {http://dx.doi.org/10.4149/gpb_2013016},
keywords = {Arrhythmia; Guinea pig; Hydroxyl radical; Rabbit; Rat},
language = {eng},
issn = {0231-5882},
journal = {General Physiology and Biophysics},
title = {Hydroxyl radicals’ production and ECG parameters during ischemia and reperfusion in rat, guinea pig and rabbit isolated heart},
volume = {32},
year = {2013}
}
TY - JOUR
ID - 1112079
AU - Paulová, Hana - Stračina, Tibor - Jarkovský, Jiří - Nováková, Marie - Táborská, Eva
PY - 2013
TI - Hydroxyl radicals’ production and ECG parameters during ischemia and reperfusion in rat, guinea pig and rabbit isolated heart
JF - General Physiology and Biophysics
VL - 32
IS - 2
SP - 221-228
EP - 221-228
PB - Veda
SN - 02315882
KW - Arrhythmia
KW - Guinea pig
KW - Hydroxyl radical
KW - Rabbit
KW - Rat
N2 - Ischemic and reperfusion injury is a serious condition related to numerous biochemical and electrical abnormalities of the myocardium. It has been repeatedly studied in various animal models. In this study, the production of hydroxyl radicals and electrophysiological parameters were compared in three species. Rat, guinea pig and rabbit isolated hearts were perfused according to Langendorff under strictly identical conditions. The heart rate and arrhythmia were monitored during ischemia and reperfusion periods at defined time intervals; the production of hydroxyl radical was determined by HPLC as 2.5-dihydroxybenzoic acid (2.5-DHBA) formed by salicylic acid hydroxylation. Relationship between arrhythmias and production of 2.5-DHBA was studied. The inter-species differences were observed in timing of arrhythmias onset and their severity, and in the production of 2.5-DHBA in both ischemia and reperfusion. The most considerable changes were observed in rats, where arrhythmias appeared early and with highest severity during ischemia on one side and the regular rhythm was restored early and completely during reperfusion. The corresponding changes in the production of 2.5-DHBA were observed. It can be concluded that rat isolated heart is the most suitable model for evaluation of ischemia/reperfusion injury under given experimental conditions.
ER -
PAULOVÁ, Hana, Tibor STRAČINA, Jiří JARKOVSKÝ, Marie NOVÁKOVÁ and Eva TÁBORSKÁ. Hydroxyl radicals’ production and ECG parameters during ischemia and reperfusion in rat, guinea pig and rabbit isolated heart. \textit{General Physiology and Biophysics}. Bratislava: Veda, 2013, vol.~32, No~2, p.~221-228. ISSN~0231-5882. Available from: https://dx.doi.org/10.4149/gpb\_{}2013016.
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