2013
Hydroxyl radicals’ production and ECG parameters during ischemia and reperfusion in rat, guinea pig and rabbit isolated heart
PAULOVÁ, Hana, Tibor STRAČINA, Jiří JARKOVSKÝ, Marie NOVÁKOVÁ, Eva TÁBORSKÁ et. al.Základní údaje
Originální název
Hydroxyl radicals’ production and ECG parameters during ischemia and reperfusion in rat, guinea pig and rabbit isolated heart
Autoři
PAULOVÁ, Hana (203 Česká republika, garant, domácí), Tibor STRAČINA (703 Slovensko, domácí), Jiří JARKOVSKÝ (203 Česká republika, domácí), Marie NOVÁKOVÁ (203 Česká republika, domácí) a Eva TÁBORSKÁ (203 Česká republika, domácí)
Vydání
General Physiology and Biophysics, Bratislava, Veda, 2013, 0231-5882
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Slovensko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.875
Kód RIV
RIV/00216224:14110/13:00068604
Organizační jednotka
Lékařská fakulta
UT WoS
000320092300008
Klíčová slova anglicky
Arrhythmia; Guinea pig; Hydroxyl radical; Rabbit; Rat
Příznaky
Recenzováno
Změněno: 22. 4. 2014 16:12, Soňa Böhmová
Anotace
V originále
Ischemic and reperfusion injury is a serious condition related to numerous biochemical and electrical abnormalities of the myocardium. It has been repeatedly studied in various animal models. In this study, the production of hydroxyl radicals and electrophysiological parameters were compared in three species. Rat, guinea pig and rabbit isolated hearts were perfused according to Langendorff under strictly identical conditions. The heart rate and arrhythmia were monitored during ischemia and reperfusion periods at defined time intervals; the production of hydroxyl radical was determined by HPLC as 2.5-dihydroxybenzoic acid (2.5-DHBA) formed by salicylic acid hydroxylation. Relationship between arrhythmias and production of 2.5-DHBA was studied. The inter-species differences were observed in timing of arrhythmias onset and their severity, and in the production of 2.5-DHBA in both ischemia and reperfusion. The most considerable changes were observed in rats, where arrhythmias appeared early and with highest severity during ischemia on one side and the regular rhythm was restored early and completely during reperfusion. The corresponding changes in the production of 2.5-DHBA were observed. It can be concluded that rat isolated heart is the most suitable model for evaluation of ischemia/reperfusion injury under given experimental conditions.
Návaznosti
| MSM0021622402, záměr |
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| MUNI/A/0818/2012, interní kód MU |
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| MUNI/A/0951/2012, interní kód MU |
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