Detailed Information on Publication Record
2013
Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
VOJTEK, Libor, Pavel DOBEŠ, Ender BÜYÜKGÜZEL and Pavel HYRŠLBasic information
Original name
Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
Name in Czech
Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
Authors
VOJTEK, Libor (203 Czech Republic, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Ender BÜYÜKGÜZEL (792 Turkey) and Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution)
Edition
Darmstadt, Insect pathogens and entomoparasitic nematodes, p. 333-338, 6 pp. 2013
Publisher
IOBC-WPRS 2013
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
30102 Immunology
Country of publisher
Croatia
Confidentiality degree
není předmětem státního či obchodního tajemství
Publication form
printed version "print"
RIV identification code
RIV/00216224:14310/13:00065681
Organization unit
Faculty of Science
ISBN
978-92-9067-268-5
Keywords (in Czech)
antibacterial activity; Galleria mellonella; Bombyx mori; bioluminescent bacteria
Keywords in English
antibacterial activity; Galleria mellonella; Bombyx mori; bioluminescent bacteria
Tags
International impact, Reviewed
Změněno: 30/1/2016 23:25, Mgr. Pavel Dobeš, Ph.D.
V originále
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates.
In Czech
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates.
Links
| QJ1210047, research and development project |
|