VOJTEK, Libor, Pavel DOBEŠ, Ender BÜYÜKGÜZEL and Pavel HYRŠL. Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph. In Insect pathogens and entomoparasitic nematodes. Darmstadt: IOBC-WPRS 2013, 2013, p. 333-338. ISBN 978-92-9067-268-5.
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Basic information
Original name Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
Name in Czech Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
Authors VOJTEK, Libor (203 Czech Republic, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Ender BÜYÜKGÜZEL (792 Turkey) and Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution).
Edition Darmstadt, Insect pathogens and entomoparasitic nematodes, p. 333-338, 6 pp. 2013.
Publisher IOBC-WPRS 2013
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 30102 Immunology
Country of publisher Croatia
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
RIV identification code RIV/00216224:14310/13:00065681
Organization unit Faculty of Science
ISBN 978-92-9067-268-5
Keywords (in Czech) antibacterial activity; Galleria mellonella; Bombyx mori; bioluminescent bacteria
Keywords in English antibacterial activity; Galleria mellonella; Bombyx mori; bioluminescent bacteria
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavel Dobeš, Ph.D., učo 150960. Changed: 30/1/2016 23:25.
Abstract
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates.
Abstract (in Czech)
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates.
Links
QJ1210047, research and development projectName: Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění (Acronym: Probiotika - imunita včel)
Investor: Ministry of Agriculture of the CR
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