Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium
from Antarctica

J 2013

Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica

KOSINA, Marcel; Miloš BARTÁK; Ivana MAŠLAŇOVÁ; Andrea VÁVROVÁ PASCUTTI; Ondrej ŠEDO et al.

Základní údaje

Originální název

Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica

Autoři

KOSINA, Marcel; Miloš BARTÁK; Ivana MAŠLAŇOVÁ; Andrea VÁVROVÁ PASCUTTI; Ondrej ŠEDO; Matej LEXA a Ivo SEDLÁČEK

Vydání

Current Microbiology, Springer US, 2013, 0343-8651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.359

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/13:00068676

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1007/s00284-013-0406-6

UT WoS

000325813400001

Klíčová slova anglicky

Antarctic; Pseudomonas; Taxonomy; rpoB; rrs; rpoD

Štítky

AKR, podil, rivok

Změněno: 13. 3. 2018 14:21, doc. Ing. Matej Lexa, Ph.D.

Anotace

V originále

During Czech expeditions at James Ross Island, Antarctica, in the years 2007–2009, the bacterial diversity of the genus Pseudomonas was studied. Twelve fluorescent Pseudomonas strains were isolated from various samples and were subjected to a detailed taxonomic study. A polyphasic approach included genotypic and phenotypic analyses. The genotypic analysis involved sequencing of rrs, rpoB and rpoD genes, DNA–DNA hybridization (DDH) studies as well as manual ribotyping using HindIII endonuclease. The phenotypic characterization included conventional tests as well as biotyping using the Biolog system, protein profiling by SDS-PAGE, and MALDI-TOF MS analysis. Our taxonomic study revealed that all isolates belonged to the same Pseudomonas species with psychrotrophic growth not exceeding 37C. The cultures showed a unique position among the phylogenetically related pseudomonads. DDH experiment between the proposed type strain of the antarctic isolates and the closest neighbour P. arsenicoxydans CCM 8423T showed only 40.9–50.1 % similarity, thus confirming that the characterized strains do not belong to the P. arsenicoxydans species. According to the results obtained we propose the name P. prosekii sp. nov. for this novel Pseudomonas taxon with type strain AN/28/1T (=CCM 7990T and LMG 26867T).

Návaznosti

CZ.1.05/1.1.00/02.0068, interní kód MU

Název: CEITEC - středoevropský technologický institut (Akronym: CEITEC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC - středoevropský technologický institut, 1.1 Evropská centra excelence

LM2010009, projekt VaV

Název: Projekt CzechPolar - České polární stanice: Stavba a operační náklady

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar - České polární stanice: Stavba a operační náklady

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Přiložené soubory

ZVV_2013_32_1113275.pdf

Požádat o autorskou verzi souboru

Citovat

KOSINA, Marcel; Miloš BARTÁK; Ivana MAŠLAŇOVÁ; Andrea VÁVROVÁ PASCUTTI; Ondrej ŠEDO; Matej LEXA a Ivo SEDLÁČEK. Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica. Current Microbiology. Springer US, 2013, roč. 67, č. 6, s. 637-646. ISSN 0343-8651. Dostupné z: https://doi.org/10.1007/s00284-013-0406-6.

@article{1113275,
   author = {Kosina, Marcel and Barták, Miloš and Mašlaňová, Ivana and Vávrová Pascutti, Andrea and Šedo, Ondrej and Lexa, Matej and Sedláček, Ivo},
   article_number = {6},
   doi = {https://doi.org/10.1007/s00284-013-0406-6},
   keywords = {Antarctic; Pseudomonas; Taxonomy; rpoB; rrs; rpoD},
   language = {eng},
   issn = {0343-8651},
   journal = {Current Microbiology},
   title = {Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica},
   url = {http://link.springer.com/article/10.1007%2Fs00284-013-0406-6},
   volume = {67},
   year = {2013}
}

TY  - JOUR
ID  - 1113275
AU  - Kosina, Marcel - Barták, Miloš - Mašlaňová, Ivana - Vávrová Pascutti, Andrea - Šedo, Ondrej - Lexa, Matej - Sedláček, Ivo
PY  - 2013
TI  - Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica
JF  - Current Microbiology
VL  - 67
IS  - 6
SP  - 637-646
EP  - 637-646
PB  - Springer US
SN  - 03438651
KW  - Antarctic
KW  - Pseudomonas
KW  - Taxonomy
KW  - rpoB
KW  - rrs
KW  - rpoD
UR  - http://link.springer.com/article/10.1007%2Fs00284-013-0406-6
L2  - http://link.springer.com/article/10.1007%2Fs00284-013-0406-6
N2  - During Czech expeditions at James Ross Island, Antarctica, in the years 2007–2009, the bacterial diversity of the genus Pseudomonas was studied. Twelve fluorescent Pseudomonas strains were isolated from various samples and were subjected to a detailed taxonomic study. A polyphasic approach included genotypic and phenotypic analyses. The genotypic analysis involved sequencing of rrs, rpoB and rpoD genes, DNA–DNA hybridization (DDH) studies as well as manual ribotyping using HindIII endonuclease. The phenotypic characterization included conventional tests as well as biotyping using the Biolog system, protein profiling by SDS-PAGE, and MALDI-TOF MS analysis. Our taxonomic study revealed that all isolates belonged to the same Pseudomonas species with psychrotrophic growth not exceeding 37C. The cultures showed a unique position among the phylogenetically related pseudomonads. DDH experiment between the proposed type strain of the antarctic isolates and the closest neighbour P. arsenicoxydans CCM 8423T showed only 40.9–50.1 % similarity, thus confirming that the characterized strains do not belong to the P. arsenicoxydans species. According to the results obtained we propose the name P. prosekii sp. nov. for this novel Pseudomonas taxon with type strain AN/28/1T (=CCM 7990T and LMG 26867T).
ER  -

KOSINA, Marcel; Miloš BARTÁK; Ivana MAŠLAŇOVÁ; Andrea VÁVROVÁ PASCUTTI; Ondrej ŠEDO; Matej LEXA a Ivo SEDLÁČEK. Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica. \textit{Current Microbiology}. Springer US, 2013, roč.~67, č.~6, s.~637-646. ISSN~0343-8651. Dostupné z: https://doi.org/10.1007/s00284-013-0406-6.

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