Staphylococcus spp. inhabiting backyard rabbits

a 2013

Staphylococcus spp. inhabiting backyard rabbits

ŠVEC, Pavel, Oto MELTER, Jan TKADLEC a Roman PANTŮČEK

Základní údaje

Originální název

Staphylococcus spp. inhabiting backyard rabbits

Autoři

ŠVEC, Pavel (203 Česká republika, garant, domácí), Oto MELTER (203 Česká republika), Jan TKADLEC (203 Česká republika) a Roman PANTŮČEK (203 Česká republika, domácí)

Vydání

XXXII Annual Meeting of the European Culture Collections' Organization (ECCO XXXI), 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/13:00068775

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Staphylococcus spp.; backyard rabbits; taxonomy; identification; rep-PCR; MALDI TOF; biotyping

Štítky

Změněno: 28. 4. 2014 13:48, Ing. Zdeňka Rašková

Anotace

V originále

A group of 64 staphylococcal strains collected from clinically healthy backyard rabbits was investigated in order to elucidate the taxonomic structure of Staphylococcus spp. inhabiting these animals. Analysed strains were isolated from nose (30 strains), ear (24), anal (6), and throat (4) swab samples obtained from 16 weaned or adult rabbits kept in hutches by a small breeder in Spišská Nová Ves (Slovakia). Isolated strains were characterized using basic genus-specific conventional tests and further identified using a combination of biotyping, rep-PCR fingerprinting with the (GTG)5 primer and MALDI TOF mass spectrometry. Final identification of two strains was further confirmed using rpoB gene sequencing. All but two rep-PCR fingerprints clearly matched reference database entries and assigned 36 strains as Staphylococcus vitulinus, 20 as Staphylococcus xylosus, four as Staphylococcus succinus and remaining strains as single representatives of Staphylococcus sciuri, Staphylococcus equorum and Staphylococcus haemolyticus. Only one strain (S. xylosus) was not identified using this method. In contrast, MALDI TOF mass spectrometry failed to identify 22 S. vitulinus, 6 S. xylosus, 3 S. succinus and a S. haemolyticus strain. Biotyping results assigned correctly only 20 S. xylosus isolates; remaining strains were not identified or were identified erroneously. Individual animals were inhabited by one to three staphylococcal species. All four S. succinus strains were isolated only from nose samples; however no clear correlation between the species composition and the body part swabbed was found. Obtained results showed that S. vitulinus and S. xylosus species are common inhabitants of healthy rabbits and confirmed rep-PCR with (GTG)5 primer as a good tool for identification of Staphylococcus spp. This study was supported by project CEB (CZ.1.07/2.3.00/20.0183).

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

Citovat

ŠVEC, Pavel, Oto MELTER, Jan TKADLEC a Roman PANTŮČEK. Staphylococcus spp. inhabiting backyard rabbits. In XXXII Annual Meeting of the European Culture Collections' Organization (ECCO XXXI). 2013.

@proceedings{1114512,
   author = {Švec, Pavel and Melter, Oto and Tkadlec, Jan and Pantůček, Roman},
   booktitle = {XXXII Annual Meeting of the European Culture Collections' Organization (ECCO XXXI)},
   keywords = {Staphylococcus spp.; backyard rabbits; taxonomy; identification; rep-PCR; MALDI TOF; biotyping},
   language = {eng},
   title = {Staphylococcus spp. inhabiting backyard rabbits},
   year = {2013}
}

TY  - CONF
ID  - 1114512
AU  - Švec, Pavel - Melter, Oto - Tkadlec, Jan - Pantůček, Roman
PY  - 2013
TI  - Staphylococcus spp. inhabiting backyard rabbits
KW  - Staphylococcus spp.
KW  - backyard rabbits
KW  - taxonomy
KW  - identification
KW  - rep-PCR
KW  - MALDI TOF
KW  - biotyping
N2  - A group of 64 staphylococcal strains collected from clinically healthy backyard rabbits was investigated in order to elucidate the taxonomic structure of Staphylococcus spp. inhabiting these animals. Analysed strains were isolated from nose (30 strains), ear (24), anal (6), and throat (4) swab samples obtained from 16 weaned or adult rabbits kept in hutches by a small breeder in Spišská Nová Ves (Slovakia). Isolated strains were characterized using basic genus-specific conventional tests and further identified using a combination of biotyping, rep-PCR fingerprinting with the (GTG)5 primer and MALDI TOF mass spectrometry. Final identification of two strains was further confirmed using rpoB gene sequencing. All but two rep-PCR fingerprints clearly matched reference database entries and assigned 36 strains as Staphylococcus vitulinus, 20 as Staphylococcus xylosus, four as Staphylococcus succinus and remaining strains as single representatives of Staphylococcus sciuri, Staphylococcus equorum and Staphylococcus haemolyticus. Only one strain (S. xylosus) was not identified using this method. In contrast, MALDI TOF mass spectrometry failed to identify 22 S. vitulinus, 6 S. xylosus, 3 S. succinus and a S. haemolyticus strain. Biotyping results assigned correctly only 20 S. xylosus isolates; remaining strains were not identified or were identified erroneously. Individual animals were inhabited by one to three staphylococcal species. All four S. succinus strains were isolated only from nose samples; however no clear correlation between the species composition and the body part swabbed was found. Obtained results showed that S. vitulinus and S. xylosus species are common inhabitants of healthy rabbits and confirmed rep-PCR with (GTG)5 primer as a good tool for identification of Staphylococcus spp. This study was supported by project CEB (CZ.1.07/2.3.00/20.0183).
ER  -

ŠVEC, Pavel, Oto MELTER, Jan TKADLEC a Roman PANTŮČEK. Staphylococcus spp. inhabiting backyard rabbits. In \textit{XXXII Annual Meeting of the European Culture Collections' Organization (ECCO XXXI)}. 2013.

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