Whole Genome Sequence of the Treponema Fribourg-Blanc:
Unspecified Simian Isolate Is Highly Similar to the Yaws
Subspecies

J 2013

Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies

ZOBANÍKOVÁ, Marie, Michal STROUHAL, Lenka MIKALOVÁ, Darina ČEJKOVÁ, Lenka AMBROŽOVÁ et. al.

Základní údaje

Originální název

Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies

Autoři

ZOBANÍKOVÁ, Marie (203 Česká republika, domácí), Michal STROUHAL (203 Česká republika, domácí), Lenka MIKALOVÁ (203 Česká republika, domácí), Darina ČEJKOVÁ (203 Česká republika, domácí), Lenka AMBROŽOVÁ (203 Česká republika, domácí), Petra POSPÍŠILOVÁ (203 Česká republika, domácí), Lucinda L. FULTON (840 Spojené státy), Lei CHEN (840 Spojené státy), Erica SODERGREN (840 Spojené státy), George M. WEINSTOCK (840 Spojené státy) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

PLOS Neglected Tropical Diseases, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2013, 1935-2735

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.489

Kód RIV

RIV/00216224:14110/13:00065612

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pntd.0002172

UT WoS

000318153100026

Klíčová slova anglicky

PALLIDUM SSP PALLIDUM; PATHOGENIC TREPONEMES; GENE-EXPRESSION; SYPHILIS; STRAINS; CONSERVATION; PERTENUE; LIPOPROTEIN; INFECTION; DISEASE

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 8. 2013 15:26, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Background: Unclassified simian strain Treponema Fribourg-Blanc was isolated in 1966 from baboons (Papio cynocephalus)in West Africa. This strain was morphologically indistinguishable from T. pallidum ssp. pallidum or ssp. pertenue strains, and it was shown to cause human infections. Methodology/Principal Findings: To precisely define genetic differences between Treponema Fribourg-Blanc (unclassified simian isolate, FB) and T. pallidum ssp. pertenue strains (TPE), a high quality sequence of the whole Fribourg-Blanc genome was determined with 454-pyrosequencing and Illumina sequencing platforms. Combined average coverage of both methods was greater than 5006. Restriction target sites (n = 1,773), identified in silico, of selected restriction enzymes within the Fribourg-Blanc genome were verified experimentally and no discrepancies were found. When compared to the other three sequenced TPE genomes (Samoa D, CDC-2, Gauthier), no major genome rearrangements were found. The Fribourg- Blanc genome clustered with other TPE strains (especially with the TPE CDC-2 strain), while T. pallidum ssp. pallidum strains clustered separately as well as the genome of T. paraluiscuniculi strain Cuniculi A. Within coding regions, 6 deletions, 5 insertions and 117 substitutions differentiated Fribourg-Blanc from other TPEgenomes. Conclusions/Significance: The Fribourg-Blanc genome showed similar genetic characteristics as other TPE strains. Therefore, we propose to rename the unclassified simian isolate to Treponema pallidum ssp. pertenue strain Fribourg-Blanc. Since the Fribourg-Blanc strain was shown to cause experimental infection in human hosts, non-human primates could serve as possible reservoirs of TPE strains. This could considerably complicate recent efforts to eradicate yaws. Genetic differences specific for Fribourg-Blanc could then contribute for identification of cases of animal-derived yaws infections.

Návaznosti

GAP302/12/0574, projekt VaV

Název: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Grantová agentura ČR, Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

NT11159, projekt VaV

Název: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministerstvo zdravotnictví ČR, Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Přiložené soubory

zobanikova.pdf

Požádat o autorskou verzi souboru

Citovat

ZOBANÍKOVÁ, Marie, Michal STROUHAL, Lenka MIKALOVÁ, Darina ČEJKOVÁ, Lenka AMBROŽOVÁ, Petra POSPÍŠILOVÁ, Lucinda L. FULTON, Lei CHEN, Erica SODERGREN, George M. WEINSTOCK a David ŠMAJS. Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies. PLOS Neglected Tropical Diseases. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2013, roč. 7, č. 4, s. 1-8. ISSN 1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0002172.

@article{1115114,
   author = {Zobaníková, Marie and Strouhal, Michal and Mikalová, Lenka and Čejková, Darina and Ambrožová, Lenka and Pospíšilová, Petra and Fulton, Lucinda L. and Chen, Lei and Sodergren, Erica and Weinstock, George M. and Šmajs, David},
   article_location = {SAN FRANCISCO},
   article_number = {4},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0002172},
   keywords = {PALLIDUM SSP PALLIDUM; PATHOGENIC TREPONEMES; GENE-EXPRESSION; SYPHILIS; STRAINS; CONSERVATION; PERTENUE; LIPOPROTEIN; INFECTION; DISEASE},
   language = {eng},
   issn = {1935-2735},
   journal = {PLOS Neglected Tropical Diseases},
   title = {Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies},
   volume = {7},
   year = {2013}
}

TY  - JOUR
ID  - 1115114
AU  - Zobaníková, Marie - Strouhal, Michal - Mikalová, Lenka - Čejková, Darina - Ambrožová, Lenka - Pospíšilová, Petra - Fulton, Lucinda L. - Chen, Lei - Sodergren, Erica - Weinstock, George M. - Šmajs, David
PY  - 2013
TI  - Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies
JF  - PLOS Neglected Tropical Diseases
VL  - 7
IS  - 4
SP  - 1-8
EP  - 1-8
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19352735
KW  - PALLIDUM SSP PALLIDUM
KW  - PATHOGENIC TREPONEMES
KW  - GENE-EXPRESSION
KW  - SYPHILIS
KW  - STRAINS
KW  - CONSERVATION
KW  - PERTENUE
KW  - LIPOPROTEIN
KW  - INFECTION
KW  - DISEASE
N2  - Background: Unclassified simian strain Treponema Fribourg-Blanc was isolated in 1966 from baboons (Papio cynocephalus)in West Africa. This strain was morphologically indistinguishable from T. pallidum ssp. pallidum or ssp. pertenue strains, and it was shown to cause human infections. Methodology/Principal Findings: To precisely define genetic differences between Treponema Fribourg-Blanc (unclassified simian isolate, FB) and T. pallidum ssp. pertenue strains (TPE), a high quality sequence of the whole Fribourg-Blanc genome was determined with 454-pyrosequencing and Illumina sequencing platforms. Combined average coverage of both methods was greater than 5006. Restriction target sites (n = 1,773), identified in silico, of selected restriction enzymes within the Fribourg-Blanc genome were verified experimentally and no discrepancies were found. When compared to the other three sequenced TPE genomes (Samoa D, CDC-2, Gauthier), no major genome rearrangements were found. The Fribourg- Blanc genome clustered with other TPE strains (especially with the TPE CDC-2 strain), while T. pallidum ssp. pallidum strains clustered separately as well as the genome of T. paraluiscuniculi strain Cuniculi A. Within coding regions, 6 deletions, 5 insertions and 117 substitutions differentiated Fribourg-Blanc from other TPEgenomes. Conclusions/Significance: The Fribourg-Blanc genome showed similar genetic characteristics as other TPE strains. Therefore, we propose to rename the unclassified simian isolate to Treponema pallidum ssp. pertenue strain Fribourg-Blanc. Since the Fribourg-Blanc strain was shown to cause experimental infection in human hosts, non-human primates could serve as possible reservoirs of TPE strains. This could considerably complicate recent efforts to eradicate yaws. Genetic differences specific for Fribourg-Blanc could then contribute for identification of cases of animal-derived yaws infections.
ER  -

ZOBANÍKOVÁ, Marie, Michal STROUHAL, Lenka MIKALOVÁ, Darina ČEJKOVÁ, Lenka AMBROŽOVÁ, Petra POSPÍŠILOVÁ, Lucinda L. FULTON, Lei CHEN, Erica SODERGREN, George M. WEINSTOCK a David ŠMAJS. Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies. \textit{PLOS Neglected Tropical Diseases}. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2013, roč.~7, č.~4, s.~1-8. ISSN~1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0002172.

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