KREJČOVÁ, Ludmila, David HYNEK, Pavel KOPEL, Vojtech ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ and René KIZEK. Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs. Chromatographia. 2013, vol. 76, 7-8, p. 355-362. ISSN 0009-5893. Available from: https://dx.doi.org/10.1007/s10337-012-2327-0.
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Basic information
Original name Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs
Authors KREJČOVÁ, Ludmila, David HYNEK, Pavel KOPEL, Vojtech ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ and René KIZEK.
Edition Chromatographia, 2013, 0009-5893.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10403 Physical chemistry
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.370
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s10337-012-2327-0
UT WoS 000316819600008
Keywords in English Biosensors; Voltammetry; Automated separation; Nanoparticles; Quantum dots; Hybridization; Virus
Tags AKR, rivok, ZR
Changed by Changed by: Ing. Zdeňka Rašková, učo 140529. Changed: 28/4/2014 14:31.
Abstract
In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine-adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 A degrees C, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 mu g mL(-1). The detection limit (3 signal/noise) was estimated as 15 ng mL(-1) of ODN-SH-Cd.
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