Immune mechanisms in periodontitis – effect of IL-4 gene
polymorphisms on cytokine production

a 2013

Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production

BÁRTOVÁ, Jiřina, Petra BOŘILOVÁ LINHARTOVÁ, Štěpán PODZIMEK, Kazi SVOBODOVÁ, Taťjana JANATOVÁ et. al.

Basic information

Original name

Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production

Name (in English)

Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production

Authors

BÁRTOVÁ, Jiřina, Petra BOŘILOVÁ LINHARTOVÁ, Štěpán PODZIMEK, Kazi SVOBODOVÁ, Taťjana JANATOVÁ, Antonín FASSMANN, Jana DUŠKOVÁ and Lydie IZAKOVIČOVÁ HOLLÁ

Edition

15th International Congress of Immunology, Milan, Italy, 2013

Other information

Type of outcome

Konferenční abstrakt

Confidentiality degree

není předmětem státního či obchodního tajemství

Keywords in English

Periodontitis, immune mechanisms, production of cytokines, IL-4, IL-4 gene polymorphismus

Abstract

V originále

Introduction: Periodontitis is a chronic inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria and immune mechanisms play important roles. The aim of this study was to assess the influence of IL-4 gene polymorphisms on cytokine production Methods: Cytokine production (IL-1alpha, IL-1beta, IL-4, IL-5, IL-6, IL-8 , IL-10, TNF–alpha, INF–gamma) was studied after in vitro stimulation of isolated peripheral blood mononuclear cells (PBMC) by mitogens and dental plaque bacteria (Aggregatibacter actinomycetemcomitans, Escherichia coli, Tannerrella forsythensis, Porphyromonas gingivalis and Heat Shock Protein 60). Cytokine production was detected by multiplex cytokine analysis system Luminex. The results were correlated with IL-4 genotypes in patients with chronic periodontitis (CP) and healthy controls. Results: We found that mononuclear cells isolated from peripheral blood of CP patients with investigated IL-4 polymorphisms (-589C/T, -33C/T, VNTR in intron 3) significantly changed production of IFN-gamma, IL-10, IL-1alpha and IL-1beta, TNF-alpha, IL-10, MCP-1, IL-6 after stimulation by all selected bacteria and HSP (p<0.05). Conclusion: In patients with CP, IL-4 polymorphisms may influence the function of mononuclear cells to produce not only IL-4 but also other cytokines. The results of this study support Seymour’s hypothesis (1999) about the role of TH2 produced cytokines such as IL-4 in genetic predisposition to chronic periodontitis.

Links

NT11405, research and development project

Name: Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu

Investor: Ministry of Health of the CR

Citovat

BÁRTOVÁ, Jiřina, Petra BOŘILOVÁ LINHARTOVÁ, Štěpán PODZIMEK, Kazi SVOBODOVÁ, Taťjana JANATOVÁ, Antonín FASSMANN, Jana DUŠKOVÁ and Lydie IZAKOVIČOVÁ HOLLÁ. Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production. In 15th International Congress of Immunology, Milan, Italy. 2013.

@proceedings{1120477,
   author = {Bártová, Jiřina and Bořilová Linhartová, Petra and Podzimek, Štěpán and Svobodová, Kazi and Janatová, Taťjana and Fassmann, Antonín and Dušková, Jana and Izakovičová Hollá, Lydie},
   booktitle = {15th International Congress of Immunology, Milan, Italy},
   keywords = {Periodontitis, immune mechanisms, production of cytokines, IL-4, IL-4 gene polymorphismus},
   title = {Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production},
   year = {2013}
}

TY  - CONF
ID  - 1120477
AU  - Bártová, Jiřina - Bořilová Linhartová, Petra - Podzimek, Štěpán - Svobodová, Kazi - Janatová, Taťjana - Fassmann, Antonín - Dušková, Jana - Izakovičová Hollá, Lydie
PY  - 2013
TI  - Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production
KW  - Periodontitis, immune mechanisms, production of cytokines, IL-4, IL-4 gene polymorphismus
N2  - Introduction: Periodontitis is a chronic inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria and immune mechanisms play important roles. The aim of this study was to assess the influence of IL-4 gene polymorphisms on cytokine production Methods: Cytokine production (IL-1alpha, IL-1beta, IL-4, IL-5, IL-6, IL-8 , IL-10, TNF–alpha, INF–gamma) was studied after in vitro stimulation of isolated peripheral blood mononuclear cells (PBMC) by mitogens and dental plaque bacteria (Aggregatibacter actinomycetemcomitans, Escherichia coli, Tannerrella forsythensis, Porphyromonas gingivalis and Heat Shock Protein 60). Cytokine production was detected by multiplex cytokine analysis system Luminex. The results were correlated with IL-4 genotypes in patients with chronic periodontitis (CP) and healthy controls. Results: We found that mononuclear cells isolated from peripheral blood of CP patients with investigated IL-4 polymorphisms (-589C/T, -33C/T, VNTR in intron 3) significantly changed production of IFN-gamma, IL-10, IL-1alpha and IL-1beta, TNF-alpha, IL-10, MCP-1, IL-6 after stimulation by all selected bacteria and HSP (p<0.05). Conclusion: In patients with CP, IL-4 polymorphisms may influence the function of mononuclear cells to produce not only IL-4 but also other cytokines. The results of this study support Seymour’s hypothesis (1999) about the role of TH2 produced cytokines such as IL-4 in genetic predisposition to chronic periodontitis.
ER  -

BÁRTOVÁ, Jiřina, Petra BOŘILOVÁ LINHARTOVÁ, Štěpán PODZIMEK, Kazi SVOBODOVÁ, Taťjana JANATOVÁ, Antonín FASSMANN, Jana DUŠKOVÁ and Lydie IZAKOVIČOVÁ HOLLÁ. Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production. In \textit{15th International Congress of Immunology, Milan, Italy}. 2013.

Detailed Information on Publication Record