| STEJSKAL, Karel, David POTĚŠIL a Zbyněk ZDRÁHAL. Suppression of Peptide Sample Losses in Autosampler Vials. Journal of Proteome Research. Washington, USA: ACS, 2013, roč. 12, č. 6, s. 3057-3062. ISSN 1535-3893. Dostupné z: https://dx.doi.org/10.1021/pr400183v. |
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@article{1120957,
author = {Stejskal, Karel and Potěšil, David and Zdráhal, Zbyněk},
article_location = {Washington, USA},
article_number = {6},
doi = {http://dx.doi.org/10.1021/pr400183v},
keywords = {MASS-SPECTROMETRY ANALYSIS; LIQUID-CHROMATOGRAPHY; RECOVERY; PROTEIN; QUANTIFICATION; OPTIMIZATION; PURIFICATION; SURFACTANTS; ADSORPTION; PROTEOMICS},
language = {eng},
issn = {1535-3893},
journal = {Journal of Proteome Research},
title = {Suppression of Peptide Sample Losses in Autosampler Vials},
url = {http://pubs.acs.org/doi/pdf/10.1021/pr400183v},
volume = {12},
year = {2013}
}
TY - JOUR
ID - 1120957
AU - Stejskal, Karel - Potěšil, David - Zdráhal, Zbyněk
PY - 2013
TI - Suppression of Peptide Sample Losses in Autosampler Vials
JF - Journal of Proteome Research
VL - 12
IS - 6
SP - 3057-3062
EP - 3057-3062
PB - ACS
SN - 15353893
KW - MASS-SPECTROMETRY ANALYSIS
KW - LIQUID-CHROMATOGRAPHY
KW - RECOVERY
KW - PROTEIN
KW - QUANTIFICATION
KW - OPTIMIZATION
KW - PURIFICATION
KW - SURFACTANTS
KW - ADSORPTION
KW - PROTEOMICS
UR - http://pubs.acs.org/doi/pdf/10.1021/pr400183v
L2 - http://pubs.acs.org/doi/pdf/10.1021/pr400183v
N2 - Protein or peptide sample losses could accompany all steps of the proteomic analysis workflow. We focused on suppression of sample adsorptive losses during sample storage in autosampler vials. We examined suppression capabilities of six different sample injection solutions and seven types of autosampler vial surfaces using a model sample (tryptic digest of six proteins, 1 fmol per protein). While the vial material did not play an essential role, the choice of appropriate composition of sample injection solution reduced adsorptive losses substantially. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (6 M and 1 M) as injection solutions (both acidified with formic acid (FA) (0.1%)) provided the best results in terms of number of significantly identified peptides (p < 0.05). These conclusions were confirmed by analyses of a real sample with intermediate complexity (in-gel digest from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)). Addition of PEG into the real sample solution proved to prevent higher losses, concerning mainly hydrophobic peptides, during up to 48 h storage in the autosampler in comparison with a formic acid solution and even with a solution of highly concentrated urea and thiourea. Using PEG for several months was not accompanied by any adverse effect to the liquid chromatography system.
ER -
STEJSKAL, Karel, David POTĚŠIL a Zbyněk ZDRÁHAL. Suppression of Peptide Sample Losses in Autosampler Vials. \textit{Journal of Proteome Research}. Washington, USA: ACS, 2013, roč.~12, č.~6, s.~3057-3062. ISSN~1535-3893. Dostupné z: https://dx.doi.org/10.1021/pr400183v.
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